Dip‐Pen Nanolithography of Bioactive Peptides on Collagen‐Terminated Retinal Membrane

Sistiabudi, Rizaldi; Ivanisevic, Albena

doi:10.1002/adma.200800950

Cited by 28 publications

(28 citation statements)

References 14 publications

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“…Initially CBP, a molecule proven to adhere to the membrane (Sistiabudi and Ivanisevic 2008), was patterned to the tissue substrate. This was followed by laminin, fibronectin, and an ECM mix.…”

Section: Resultsmentioning

confidence: 99%

“…This is of particular interest as it would facilitate studies for controlling cell morphology and cell-substrate interaction upon a natural substrate. Recently our group showed that direct patterning could be performed on a biologically derived substrate with collagen-binding peptide (Sistiabudi and Ivanisevic 2008). Patterning to this substrate is a complex problem as the substrate is inherently heterogeneous (both chemically and physically), generally soft and hydrophobic after drying (Sistiabudi and Ivanisevic 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Transport of the ink to the substrate is primarily carried out through diffusion via a water meniscus which forms at the probe substrate interface (Piner et al 1999). Transport of the ink to the substrate can be controlled through a variety of parameters including relative humidity (RH), write speed, contact force, and tip-coating (Sistiabudi and Ivanisevic 2008). Recent advancements to control ink diffusion have added secondary transport molecules including Tween-20 (Jung et al 2004) and argarose (Senesi et al 2009) to control diffusion to the substrate.…”

Section: Introductionmentioning

confidence: 99%

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Dip‐pen nanolithography on SiO_x and tissue‐derived substrates: comparison with multiple biological inks

Kramer¹,

Park²,

Ivanisevic³

2009

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There has been extensive interest in the micro and nanoscale manipulation of various substrates in the past few decades. One promising technique is dip-pen nanolithography which has shown the capability to pattern substrates of all forms including, tissue-derived substrates. Patterning of tissue-derived substrates is of particular interest, as it would facilitate studies into controlling cell morphology and cell-substrate interaction. To expand the field into this area both peptides and bioactive collagen-binding peptide-linked biomolecules were patterned to the inner collagenous zone of the Bruch's membrane (BM). Collagen-binding peptide, and extra cellular matrix (ECM) proteins laminin and fibronectin were patterned on the BM and SiO(x). The lithographic protocol was facilitated by Triton X-100 which was used to clean the tissue-derived construct after harvesting. This produced a collagen-exposed BM which was more hydrophilic (contact angle 67 degrees +/-8.49 degrees) surface compared with other cleaning methods but it maintained similar surface roughness (root-mean-square) 80+/-18 nm and collagen exposure. This type of surface can be readily patterned with the chosen inks under lower humidity conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dip‐pen nanolithography on SiO_x and tissue‐derived substrates: comparison with multiple biological inks

Kramer¹,

Park²,

Ivanisevic³

2009

Scanning

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“…92 The non-destructive nature of DPN means it is compatible with many substrates including soft, flexible polymers 93 and even biological tissue. 94 Although AFM based lithography has traditionally been regarded as a serial, and therefore slow fabrication technique, great strides have been made in parallelization of DPN; first to "massively parallel" 55000 cantilever arrays, 95 and more recently using polymer pen lithography (PPL) arrays capable of simultaneously printing up to 11 million patterns over cm 2 areas. 96 DPN is already finding use as a tool for fabricating novel nano-patterned cell-growth platforms for fundamental cell-biology studies.…”

Section: Dip-pen Nanolithographymentioning

confidence: 99%

Nano-bioelectronics via dip-pen nanolithography

et al. 2015

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The emerging field of nano-biology is borne from advances in our ability to control the structure of materials on finer and finer length-scales, coupled with an increased appreciation of the sensitivity of living cells to nanoscale topographical, chemical and mechanical cues. As we envisage and prototype nanostructured bioelectronic devices there is a crucial need to understand how cells feel and respond to nanoscale materials, particularly as material properties (surface energy, conductivity etc.) can be very different at the nanoscale than at the bulk. However, the patterning of organic bioelectronic materials is often not achievable using conventional fabrication techniques, especially on soft, biocompatible substrates. Nonconventional nanofabrication strategies are required. Dip-pen nanolithography is a nanofabrication technique which uses the nanoscale tip of an atomic force microscope to direct-write functional inks. Over the past decade, the technique has evolved as uniquely capable in the realm of bio-nanofabrication, with the capability to deposit both biomolecules and electrode materials. This review highlights this new tool for fabricating nanoscale bioelectronic devices, and for enabling heretofore unrealised experiments in the response of living cells to tailored nano-environments. We firstly introduce bioelectronics, followed by a survey of different lithography methods and their use to achieve paradigmic bioelectronic architectures. We then focus on dip-pen nanolithography, highlighting the range of bioelectronic materials and biomolecules which can be deposited using the technique, as well as its demonstrated use as a lithography tool in nano-biology. We discuss the progress made towards upscaling the DPN technology towards larger areas, in particular via the polymer pen approach. The emerging field of nano-biology is borne from advances in our ability to control the structure of materials on finer and finer length-scales, coupled with an increased appreciation of the sensitivity of living cells to nanoscale topographical, chemical and mechanical cues. As we envisage and prototype nanostructured bioelectronic devices there is a crucial need to understand how cells feel and respond to nanoscale materials, particularly as material properties (surface energy, conductivity etc.) can be very different at the nanoscale than at the bulk. However, the patterning of organic bioelectronic materials is often not achievable using conventional fabrication techniques, especially on soft, biocompatible substrates. Nonconventional nanofabrication strategies are required. Dip-pen nanolithography is a nanofabrication technique which uses the nanoscale tip of an atomic force microscope to direct-write functional inks. Over the past decade, the technique has evolved as uniquely capable in the realm of bio-nanofabrication, with the capability to deposit both biomolecules and electrode materials. This review highlights this new tool for fabricating nanoscale bioelectronic devices, and for enabling heretofore u...

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“…This AFM-based ink deposition process is generally referred to as dip pen nanolithography (DPN) 18 and enables patterning of a wide range of materials ("inks") including small organic molecules, 19 biomolecules, 20 metal nanoparticles, 21,22 and conducting polymers. 23−25 Its range of feature size resolution (from tens of nanometers up to several micrometers), ability to simultaneously pattern multiple inks, 26 upscalability, 27 and versatility in nondestructive lithography on substrates including semiconductors, plastics, biomaterials, and even biological tissue, 28 mean DPN is a promising tool for the nanostructuring of future nanoelectronic devices.…”

Section: ■ Introductionmentioning

confidence: 99%

Vapor Phase Polymerization of EDOT from Submicrometer Scale Oxidant Patterned by Dip-Pen Nanolithography

et al. 2012

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Some of the most exciting recent advances in conducting polymer synthesis have centered around the method of vapor phase polymerization (VPP) of thin films. However, it is not known whether the VPP process can proceed using significantly reduced volumes of oxidant and therefore be implemented as part of nanolithography approach. Here, we present a strategy for submicrometer scale patterning of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) via in situ VPP. Attolitre (10 -18 L) volumes of oxidant "ink" are controllably deposited using dip-pen nanolithography (DPN). DPN patterning of the oxidant ink is facilitated by the incorporation of an amphiphilic block copolymer thickener, an additive that also assists with stabilization of the oxidant. When exposed to EDOT monomer in a VPP chamber, each deposited feature localizes the synthesis of conducting PEDOT structures of several micrometers down to 250 nm in width. PEDOT patterns are characterized by atomic force microscopy (AFM), conductive AFM, two probe electrical measurement, and micro-Raman spectroscopy, evidencing in situ vapor phase synthesis of conducting polymer at a scale (picogram) which is much smaller than that previously reported. Although the process of VPP on this scale was achieved, we highlight some of the challenges that need to be overcome to make this approach feasible in an applied setting. ABSTRACT: Some of the most exciting recent advances in conducting polymer synthesis have centered around the method of vapor phase polymerization (VPP) of thin films. However, it is not known whether the VPP process can proceed using significantly reduced volumes of oxidant and therefore be implemented as part of nanolithography approach. Here, we present a strategy for submicrometer scale patterning of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) via in situ VPP. Attolitre (10 −18 L) volumes of oxidant "ink" are controllably deposited using dip-pen nanolithography (DPN). DPN patterning of the oxidant ink is facilitated by the incorporation of an amphiphilic block copolymer thickener, an additive that also assists with stabilization of the oxidant. When exposed to EDOT monomer in a VPP chamber, each deposited feature localizes the synthesis of conducting PEDOT structures of several micrometers down to 250 nm in width. PEDOT patterns are characterized by atomic force microscopy (AFM), conductive AFM, two probe electrical measurement, and micro-Raman spectroscopy, evidencing in situ vapor phase synthesis of conducting polymer at a scale (picogram) which is much smaller than that previously reported. Although the process of VPP on this scale was achieved, we highlight some of the challenges that need to be overcome to make this approach feasible in an applied setting.

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Dip‐Pen Nanolithography of Bioactive Peptides on Collagen‐Terminated Retinal Membrane

Cited by 28 publications

References 14 publications

Dip‐pen nanolithography on SiO_x and tissue‐derived substrates: comparison with multiple biological inks

Dip‐pen nanolithography on SiO_x and tissue‐derived substrates: comparison with multiple biological inks

Nano-bioelectronics via dip-pen nanolithography

Vapor Phase Polymerization of EDOT from Submicrometer Scale Oxidant Patterned by Dip-Pen Nanolithography

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Dip‐Pen Nanolithography of Bioactive Peptides on Collagen‐Terminated Retinal Membrane

Cited by 28 publications

References 14 publications

Dip‐pen nanolithography on SiOx and tissue‐derived substrates: comparison with multiple biological inks

Dip‐pen nanolithography on SiOx and tissue‐derived substrates: comparison with multiple biological inks

Nano-bioelectronics via dip-pen nanolithography

Vapor Phase Polymerization of EDOT from Submicrometer Scale Oxidant Patterned by Dip-Pen Nanolithography

Contact Info

Product

Resources

About

Dip‐pen nanolithography on SiO_x and tissue‐derived substrates: comparison with multiple biological inks

Dip‐pen nanolithography on SiO_x and tissue‐derived substrates: comparison with multiple biological inks