Dioxin impacts on lipid metabolism of soil microbes: towards effective detection and bioassessment strategies

Mahfouz, Sabrin; Mansour, Ghaytha; Murphy, Denis J.; Hanano, Abdulsamie

doi:10.1186/s40643-020-00347-1

Cited by 12 publications

(6 citation statements)

References 212 publications

(236 reference statements)

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“…Soil microbes respond to dioxin exposure by a set of biological modulations that impact their diversity and functionality. In this context, multiple parameters, e.g., density, diversity and enzymatic activities, are now used as indicators for monitoring and assessing the exposure levels of soil microbial communities to stressors (Yao et al, 2018;Mahfouz et al, 2020). Consequently, diverse bacterial and fungal species were identified and characterized as potential biodegraders of dioxins (Magan et al, 2010;Stella et al, 2017;Hanano et al, 2019a).…”

Section: Polychlorinatedmentioning

confidence: 99%

Compositional, genetic and functional characterization of soil culturable microbial communities in polychlorinated dibenzo-p-dioxins/furans contaminated soil

Mahfouz

Mansour²,

Hanano³

2022

Front. Environ. Sci.

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Dioxins (PCDD/Fs) are one of the most toxic environmental pollutants known to date. Due to their structural stability and extreme hydrophobicity dioxins persist in the ecosystems and can be bioaccumulated to critical levels in both human and animal food chains. Soils are the most important reservoirs of dioxins, thus soil microbes are highly exposed to dioxins, impacting their diversity, genetics and functional characteristics. To experimentally evaluate these effects, the diversity and functionality of soil microbes were assessed in seven local sites potentially exposed to PCDD/Fs.Concentration of dioxins in soils samples was firstly determined and the soils cultivable microbes were identified and molecularly characterized as a function of their in vitro ability to degrade the TCDD. Our results revealed that the diversity of microbial communities largely varied among the sites and was likely inversely proportional to their level of contamination with PCDD/Fs. Furthermore, the genetics profiling of dioxin-degrading bacteria revealed that the Cytochrome P450 CYPBM3-positive species largely belong to the genus Bacillus and were randomly distributed among the soils samples, while the angular dioxygenase (AD)-positive species were mainly found in highly polluted soils with a major presence of the genus Pseudomonas. Finally, the functionality of dioxin-biodegrading genes (AD or CYPBM3), was confirmed by the ability of bacteria to consume 2,3,7,8-TCDD, and this was synchronized with an induced level of both pathways. Our results suggest that different dioxin-metabolizing pathways exist under the same environmental conditions and work differentially for an effective removal of PCDD/Fs.

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Section: Polychlorinatedmentioning

confidence: 99%

Compositional, genetic and functional characterization of soil culturable microbial communities in polychlorinated dibenzo-p-dioxins/furans contaminated soil

Mahfouz

Mansour²,

Hanano³

2022

Front. Environ. Sci.

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“…9 In addition, the accidental or deliberate incineration of forest-derived and other solid materials releases considerable amounts of dioxins into the environment. 10,11 In terms of their downstream exposure pathways, dioxins tend to persist in the environment and bioaccumulate in both human and animal food chains, and this is due to their high structural stability and hydrophobicity. 3 This means that humans are mainly exposed to dioxins through the consumption of dioxin-contaminated food, which constitutes a potential health risk.…”

Section: Introductionmentioning

confidence: 99%

“…[15][16][17] In term of toxicity, dioxins are known to cause irreversible biological damage in humans, animals, plants and microorganisms, leading to wider ecological, environmental and economic impacts. 10,11,[18][19][20][21][22][23][24][25][26][27][28][29][30][31] In mammals, the major toxicological effects of dioxins are mediated by the aryl hydrocarbon receptor (AhR), a member of the cytoplasmic receptors family that act as transcription factor. 32 In this fashion, the toxicity of dioxins is related to their affinity for AhR, with 2,3,7,8-TCDD being the most potent AhR agonist.…”

Section: Introductionmentioning

confidence: 99%

“…In modern settings, dioxins are mainly produced by industrial, municipal and domestic incineration as well as during industrial processes involving chlorinated aromatic and aliphatic compounds, such as pesticides and herbicides synthesis 9 . In addition, the accidental or deliberate incineration of forest‐derived and other solid materials releases considerable amounts of dioxins into the environment 10,11 …”

Section: Introductionmentioning

confidence: 99%

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Female‐to‐male differential transcription patterns of miRNA‐mRNA networks in the livers of dioxin‐exposed mice

Hammoudeh

Soukkarieh

Murphy

et al. 2023

Environmental Toxicology

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Non‐coding microRNAs (miRNAs) have important roles in regulating the expression of liver mRNAs in response to xenobiotic‐exposure, but their roles concerning dioxins such as TCDD (2,3,7,8‐Tetrachlorodibenzo‐p‐dioxin) are less clear. This report concerns the potential implication of liver (class I) and circulating (class II) miRNAs in hepatotoxicity of female and male mice after acute exposure to TCDD. The data show that, of a total of 38 types of miRNAs, the expression of eight miRNAs were upregulated in both female and male mice exposed to TCDD. Inversely, the expression of nine miRNAs were significantly downregulated in both animal genders. Moreover, certain miRNAs were preferentially induced in either females or males. The potential downstream regulatory effects of miRNAs on their target genes was evaluated by determining the expression of three group of genes that are potentially involved in cancer biogenesis, other diseases and in hepatotoxicity. It was found that certain cancer‐related genes were more highly expressed females rather than males after exposure to TCDD. Furthermore, a paradoxical female‐to‐male transcriptional pattern was found for several disease‐related and hepatotoxicity‐related genes. These results suggest the possibility of developing of new miRNA‐specific interfering molecules to address their dysfunctions as caused by TCDD.

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“…For instance, it has recently been reported that the combined effect of fire and the presence of chromium affects soil microbial communities [10], whereas toxics compounds such as dioxines or furanes are released into the environment by forest fires [11,12]. These substances can also alter the metabolism of soil microbiota [13]. However, physical alterations of soil and the changes in its chemical properties, though less studied from the point of view of its influence in soil microbiota, are critical as microbes play a key role by driving biogeochemical cycles in soils, thus influencing, for instance, soil fertility and, though indirectly, avoiding soil losses by keeping soil structure.…”

Section: Introductionmentioning

confidence: 99%

Effects of Soil Quality on the Microbial Community Structure of Poorly Evolved Mediterranean Soils

Camacho

Mora

Picazo

et al. 2022

Toxics

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Physical and chemical alterations may affect the microbiota of soils as much as the specific presence of toxic pollutants. The relationship between the microbial diversity patterns and the soil quality in a Mediterranean context is studied here to test the hypothesis that soil microbiota is strongly affected by the level of anthropogenic soil alteration. Our aim has been to determine the potential effect of organic matter loss and associated changes in soil microbiota of poorly evolved Mediterranean soils (Leptosols and Regosols) suffering anthropogenic stress (i.e., cropping and deforestation). The studied soils correspond to nine different sites which differed in some features, such as the parent material, vegetation cover, or soil use and types. A methodological approach has been used that combines the classical physical and chemical study of soils with molecular characterization of the microbial assemblages using specific primers for Bacteria, Archaea and ectomycorrhizal Fungi. In agreement with previous studies within the region, physical, chemical and biological characteristics of soils varied notably depending on these factors. Microbial biomass, soil organic matter, and moisture, decreased in soils as deforestation increased, even in those partially degraded to substitution shrubland. Major differences were observed in the microbial community structure between the mollic and rendzic Leptosols found in forest soils, and the skeletic and dolomitic Leptosols in substitute shrublands, as well as with the skeletic and dolomitic Leptosols and calcaric Regosols in dry croplands. Forest soils displayed a higher microbial richness (OTU’s number) and biomass, as well as more stable and connected ecological networks. Here, we point out how human activities such as agriculture and other effects of deforestation led to changes in soil properties, thus affecting its quality driving changes in their microbial diversity and biomass patterns. Our findings demonstrate the potential risk that the replacement of forest areas may have in the conservation of the soil’s microbiota pool, both active and passive, which are basic for the maintenance of biogeochemical processes.

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Dioxin impacts on lipid metabolism of soil microbes: towards effective detection and bioassessment strategies

Cited by 12 publications

References 212 publications

Compositional, genetic and functional characterization of soil culturable microbial communities in polychlorinated dibenzo-p-dioxins/furans contaminated soil

Compositional, genetic and functional characterization of soil culturable microbial communities in polychlorinated dibenzo-p-dioxins/furans contaminated soil

Female‐to‐male differential transcription patterns of miRNA‐mRNA networks in the livers of dioxin‐exposed mice

Effects of Soil Quality on the Microbial Community Structure of Poorly Evolved Mediterranean Soils

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