Dioclea violacea lectin modulates the gentamicin activity against multi-resistant strains and induces nefroprotection during antibiotic exposure

Santos, Valdenice F.; Araújo, Ana Carolina Justino de; Silva, Ana L.F.; Almeida, Dnane V.; Freitas, Priscilla Ramos; Santos, Ana L.E.; Rocha, B.A.M.; Garcia, Wânius; Leme, Ala Moana; Bondan, Eduardo Fernandes; Borges, Fernanda Teixeira; Cutrim, Brenda S.; Silva, Luís C. N.; Coutinho, Henrique D.M.; Teixeira, Claudener S.

doi:10.1016/j.ijbiomac.2019.09.207

Cited by 20 publications

(10 citation statements)

References 49 publications

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“…Because AGs are inexpensive and have a good bacteriostatic effects [11], they are widely used in both clinical practice and in animal husbandry as veterinary drugs and growth factors. However, AGs have obvious side effects in the human body, such as nephrotoxicity and ototoxicity [12][13][14][15][16][17][18]. When an antibiotic is administered, some of the antibiotic is excreted and can cause environmental damage, while the rest remains in the body.…”

Section: Introductionmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research.

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Section: Introductionmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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“…The C. ensiformis and D. violacea seeds were collected from plants located at Araripe, Ceará, Brazil, and DVL and ConA lectins were purified as described previously. 41,30 Briefly, seeds from C. ensiformis and D. violacea were milled to a fine powder. Subsequently, 5 g of each powder was incubated in 50 ml of 150 mM NaCl under continuous stirring at 25 • C for 4 h. After, the solubilized proteins in the supernatant were separated by centrifugation at 10,000×g at 4 • C for 20 min.…”

Section: Purification Of Cona and Dvl Lectins From Seedsmentioning

confidence: 99%

“…ConA and DVL lectins were purified to homogeneity as previously reported. 30,41 The purification chromatograms displayed two major peaks, where the first peak (PI) corresponds to unbound proteins and the second peak (PII) corresponds to the retained lectin (Figures 1A and C). After purification, ConA showed three bands on SDS-PAGE (Figure 1B) of 25.5, 14, and 12 kDa corresponding to α, β, and γ-chains, respectively.…”

Section: Purification Of Cona and Dvl Lectins From Seedsmentioning

confidence: 99%

Photosynthesis of hybrid silver‐based nanoparticles mediated lectins and evaluation of their hemagglutinating properties

Fernandes

Capo

Tofanello

et al. 2022

Biotech and App Biochem

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Lectins are carbohydrate‐binding proteins belonging to the Leguminosae family. In this family stand out proteins extracted from species belonging to Diocleinae subtribe, which includes, for example, the seed lectin from Dioclea violacea (DVL) and the jack bean lectin Concanavalin A (ConA). Here, we report the photosynthesis of silver/silver chloride nanoparticles (NPs) assisted by ConA and DVL. The syntheses were simple processes using a green‐chemistry approach. Under electron microscopy, NPs heterogeneous in size, nearly spherical and covered by a thin lectin corona, were observed. Both NPs assisted by lectins were capable to cause strong rabbit erythrocytes agglutination with the same titers of hemagglutinating activities. These results indicate that both lectins maintained their biological activities even after association with the NPs and therefore are able to interact with biological membrane carbohydrates. However, for rabbit erythrocytes treated with proteolytic enzymes were observed different titers of hemagglutinating activities, suggesting differences in the spatial arrangement of the lectins on the surface of the NPs. This study provides evidences that these hybrid lectin‐coated silver/silver chloride NPs can be used for selective recognition and interaction with membrane carbohydrates and others biotechnological applications.

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Section: Biological Activities Of Lectinsmentioning

confidence: 99%

“…Santos et al [ 56 ] published two studies recently focused on assessing lectin antimicrobial activity and its association with drugs. In the first study, the lectin extracted from the seeds of Dioclea violet (DVL) was evaluated, and although it did not present any clinical activity when evaluated alone (MIC ≥ 1024 μg/mL), DVL–gentamicin combination increased the antibiotic activity expressively, reducing the gentamicin MIC from 50.8 to 10.1 μg/mL against MDR S. aureus , and from 32 to 12.7 μg/mL against MDR E. coli [ 56 ]. In the second study, similar results were observed when Santos et al [ 57 ] evaluated the lectin extracted from Vatairea macrocarpa (VML) seeds (MIC ≥ 1024 μg/mL); furthermore, when combined with gentamicin, norfloxacin, and penicillin, an increase in antibiotic activity was also seen, with an MIC reduction of 512 to 128 μg/mL, 40.3 to 32 μg/mL, and 512 to 406.4 μg/mL, respectively, against MDR S. aureus [ 57 ].…”

Section: Biological Activities Of Lectinsmentioning

confidence: 99%

Potential Application of Combined Therapy with Lectins as a Therapeutic Strategy for the Treatment of Bacterial Infections

2021

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Antibiotic monotherapy may become obsolete mainly due to the continuous emergence of resistance to available antimicrobials, which represents a major uncertainty to human health. Taking into account that natural products have been an inexhaustible source of new compounds with clinical application, lectins are certainly one of the most versatile groups of proteins used in biological processes, emerging as a promising alternative for therapy. The ability of lectins to recognize carbohydrates present on the cell surface allowed for the discovery of a wide range of activities. Currently the number of antimicrobials in research and development does not match the rate at which resistance mechanisms emerge to an effective antibiotic monotherapy. A promising therapeutic alternative is the combined therapy of antibiotics with lectins to enhance its spectrum of action, minimize adverse effects, and reduce resistance to treatments. Thus, this review provides an update on the experimental application of antibiotic therapies based on the synergic combination with lectins to treat infections specifically caused by multidrug-resistant and biofilm-producing Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. We also briefly discuss current strategies involving the modulation of the gut microbiota, its implications for antimicrobial resistance, and highlight the potential of lectins to modulate the host immune response against oxidative stress.

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Dioclea violacea lectin modulates the gentamicin activity against multi-resistant strains and induces nefroprotection during antibiotic exposure

Cited by 20 publications

References 49 publications

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Photosynthesis of hybrid silver‐based nanoparticles mediated lectins and evaluation of their hemagglutinating properties

Potential Application of Combined Therapy with Lectins as a Therapeutic Strategy for the Treatment of Bacterial Infections

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