Dinitrosyl iron complexes with glutathione largely relieve rats of experimental endometriosis

Adamyan, LV; Burgova, E. N.; Tkachev, N. A.; Mikoyan, V. D.; Stepanyan, A. A.; Сонова, М. М.; Galkin, Andrey; Vanin, Anatoly F.

doi:10.1134/s0006350913020036

Cited by 11 publications

(8 citation statements)

References 16 publications

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“…The duration of posture-related pain manifestations [73] was measured with the help of a stop-watch. Figures 9 and 10 illustrate the changes in the mean duration of a single pain attack and the total duration of the pain attacks during the observation period (2h) (mean data from 4 animals) [74]. From Figure 9 it follows that the mean duration of a single pain attack in DNIC-treated and control rats diminished with time.…”

Section: Rats With Experimental Endometriosismentioning

confidence: 94%

“…Figure 9:The changes in the mean duration of a single pain attack (in sec) in the control and experimental groups (the total duration of the observation period was 12 days). The zero point corresponds to the onset of treatment (day 28 after surgery)[74].…”

mentioning

confidence: 99%

“…Figure 10:The changes in the total duration of the pain attacks (in sec) recorded during the 2-hour observation period. The zero point designates the onset of treatment (day 28 after surgery)[74]. Internal factors include progressive hyperalgesia resulting from additional functional innervation in endometrial implant niduses[68].…”

mentioning

confidence: 99%

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Dinitrosyl Iron Complexes with Glutathione Suppress Surgically Induced Experimental Endometriosis in Rats

Vanin¹

2015

Austin J Reprod Med Infertil

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The review describes the results of our most recent investigations into miscellaneous effects of Dinitrosyl Iron Complexes (DNIC) with Glutathione and S-Nitrosoglutathione (GS-NO) as donors of Nitric Monoxide (NO) on the development of surgically induced endometriosis in rats. Whereas DNIC induced selective suppression of the growth of endometrioid tumors in implantation niduses, GS-NO enhanced tumour growth and lowered the immune responsiveness of experimental rats. It was suggested that the selective cytotoxic action of DNIC with glutathione on endometrioid tumors is a result of DNIC decomposition by endogenous iron chelators generated by tumor cells in order to provide the latter with iron required for fast tumor growth. The nitric monoxide released from DNIC either inside tumor cells or in their vicinity is oxidized to cytotoxic peroxynitrite and thus contributes to the strictly selective cytotoxic effect of DNIC on tumor cells. Such selectivity is not specific to GS-NO whose decomposition is spontaneous and can take place in different divisions of the abdominal cavity.

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Section: Rats With Experimental Endometriosismentioning

confidence: 94%

mentioning

confidence: 99%

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Dinitrosyl Iron Complexes with Glutathione Suppress Surgically Induced Experimental Endometriosis in Rats

Vanin¹

2015

Austin J Reprod Med Infertil

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“…Another possibility should not be ruled out as well. Nitrosonium cations as DNIC components can be responsible for the ability to suppress the development of experimental endometriosis in rats, as we discovered [ 19 , 20 , 108 , 109 , 110 , 111 ]. These studies, which were very difficult to conduct, started in our laboratory in 2011–2012 when my collaborator Evgenia Burgova managed to reproduce the method for initiating experimental endometriosis in rats.…”

Section: Biological Activity Of Dnics With Thiol-containing Ligands As Donors Of No and No +mentioning

confidence: 99%

Physico-Chemistry of Dinitrosyl Iron Complexes as a Determinant of Their Biological Activity

Vanin

2021

IJMS

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In this article we minutely discuss the so-called “oxidative” mechanism of mononuclear form of dinitrosyl iron complexes (M-DNICs) formations proposed by the author. M-DNICs are proposed to be formed from their building material—neutral NO molecules, Fe2+ ions and anionic non-thiol (L−) and thiol (RS−) ligands based on the disproportionation reaction of NO molecules binding with divalent ion irons in pairs. Then a protonated form of nitroxyl anion (NO−) appearing in the reaction is released from this group and a neutral NO molecule is included instead. As a result, M-DNICs are produced. Their resonance structure is described as [(L−)2Fe2+(NO)(NO+)], in which nitrosyl ligands are represented by NO molecules and nitrosonium cations in equal proportions. Binding of hydroxyl ions with the latter causes conversion of these cations into nitrite anions at neutral pH values and therefore transformation of DNICs into the corresponding high-spin mononitrosyl iron complexes (MNICs) with the resonance structure described as [(L−)2Fe2+(NO)]. In case of replacing L− by thiol-containing ligands, which are characterized by high π-donor activity, electron density transferred from sulfur atoms to iron-dinitrosyl groups neutralizes the positive charge on nitrosonium cations, which prevents their hydrolysis, ensuring relatively a high stability of the corresponding M-DNICs with the resonance structure [(RS−)2Fe2+ (NO, NO+)]. Therefore, M-DNICs with thiol-containing ligands, as well as their binuclear analogs (B-DNICs, respective resonance structure [(RS−)2Fe2+2 (NO, NO+)2]), can serve donors of both NO and NO+. Experiments with solutions of B-DNICs with glutathione or N-acetyl-L-cysteine (B-DNIC-GSH or B-DNIC-NAC) showed that these complexes release both NO and NO+ in case of decomposition in the presence of acid or after oxidation of thiol-containing ligands in them. The level of released NO was measured via optical absorption intensity of NO in the gaseous phase, while the number of released nitrosonium cations was determined based on their inclusion in S-nitrosothiols or their conversion into nitrite anions. Biomedical research showed the ability of DNICs with thiol-containing ligands to be donors of NO and NO+ and produce various biological effects on living organisms. At the same time, NO molecules released from DNICs usually have a positive and regulatory effect on organisms, while nitrosonium cations have a negative and cytotoxic effect.

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“…Such a scenario might have taken place in the studies by a group of German investigators who examined the proapoptotic effect of DNIC with thiosulfate on human Jurkat leukemia cells [44]. In our investigations we established that DNIC with glutathione exert a similar cytotoxic effect on an endometriosis model in rats [45]. The cytotoxic effect of DNIC with glutathione was studied after their intraperitoneal injection to rats with experimental endometriosis induced by a transfer of 2-mm uterine tissue grafts to rat peritoneum.…”

Section: Introductionmentioning

confidence: 67%

In-Vitro Antibacterial Activity of Processed Moringa Oleifera Seed Extract: Kill-Time Study

Idris¹,

Muyibi²,

Jamal³

et al. 2013

TOPROCJ

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Some present-day concepts of biological activities of dinitrosyl iron complexes (DNIC) with thiol-containing ligands are reviewed. These activities are determined by the ability of DNIC to act as donors of NO and nitrosonium ions (NO + ) in various body cells and tissues. DNIC are endowed with strong vasodilator activity. Due to the latter DNIC single dose injection into animal and human organisms induces long-lasting hypotension. Moreover, DNIC suppress platelet aggregation, increase red blood cell elasticity, accelerate skin wound healing, induce penis erection in animals and exert pronounced anti-apoptotic effect on cultured non-tumor cells. These regulatory effects are due to the slow release of NO and NO + from intact DNIC at both organism and in cell cultures. Fast decomposition of DNIC, e.g., in the presence of exogenous or endogenous iron-chelating agents, results in a toxic effect of DNIC on various cells and tissues and causes apoptosis of, e.g., HeLa and Jurkat human tumor cells. More recent model studies established the ability of DNIC to suppress endometriosis in rats.

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Dinitrosyl iron complexes with glutathione largely relieve rats of experimental endometriosis

Cited by 11 publications

References 16 publications

Dinitrosyl Iron Complexes with Glutathione Suppress Surgically Induced Experimental Endometriosis in Rats

Dinitrosyl Iron Complexes with Glutathione Suppress Surgically Induced Experimental Endometriosis in Rats

Physico-Chemistry of Dinitrosyl Iron Complexes as a Determinant of Their Biological Activity

In-Vitro Antibacterial Activity of Processed Moringa Oleifera Seed Extract: Kill-Time Study

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