Dimorphism in Candida Albicans. I. Morphology- Dependent Changes in Cellular Content of Macromolecules and Respiratory Activity

Yamaguchi, Hideyo

doi:10.2323/jgam.20.87

Cited by 21 publications

(10 citation statements)

References 20 publications

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“…Contradictory results have been reported as to the respiratory activity in C. albicans in relation to its growth and morphology [5,36,54,62,[73][74][75]124). A group led by Nickersen was one of the earliest to make extensive biochemical studies of C. albicans dimorphism [73][74][75].…”

Section: Morphogenesis and Respirationmentioning

confidence: 99%

“…Studies by Yamaguchi [124] on changes in respiratory activity during yeast to mycelial transition in C. albieans showed that respiratory activity of yeast cells declined immediately upon their transfer to medium which induces mycelial growth; this decline continued throughout the culture. Similarly, hyphal formation induced by proline resulted in an inhibition of respiration similar to the glucose effect during the yeast to hypha I transition [53,54].…”

Section: Morphogenesis and Respirationmentioning

confidence: 99%

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Growth and Respiration Characteristics of Candida albicans

Anand

Prasad

1991

Candida Albicans

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Section: Morphogenesis and Respirationmentioning

confidence: 99%

Section: Morphogenesis and Respirationmentioning

confidence: 99%

Growth and Respiration Characteristics of Candida albicans

Anand

Prasad

1991

Candida Albicans

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“…Only the metabolic pathways for which there was a statistically significant change in activity (P-value < 0.05) are shown. Yamaguchi, 1974; reported that yeast respiratory activity declined immediately after cells were resuspended in a hyphae-inducing medium. They found that genes encoding enzymes involved in amino acid biosynthesis, acetyl-CoA biosynthesis and nitrogen metabolism were up-regulated.…”

Section: Discussionmentioning

confidence: 99%

“…Rozp dowska et al (2011) suggested that C. albicans does not possess an efficient glucose repression of respiration mechanism, and Veiga et al (2000) demonstrated that C. albicans can undertake cyanide-resistant respiration and therefore is often considered as a crabtree-negative yeast. Yamaguchi, 1974; reported that yeast respiratory activity declined immediately after cells were resuspended in a hyphae-inducing medium. Land et al (1975) showed that electron transfer through oxidative phosphorylation is required to maintain growth in the yeast morphology.…”

Section: Discussionmentioning

confidence: 99%

The metabolic response ofCandida albicansto farnesol under hyphae-inducing conditions

2012

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Farnesol is a quorum-sensing molecule (QSM) produced, and sensed, by the polymorphic fungus, Candida albicans. This cell-to-cell communication molecule is known to suppress the hyphal formation of C. albicans at high cell density. Despite many studies investigating the signalling mechanisms by which QSMs influence the morphogenesis of C. albicans, the downstream metabolic effect of these signalling pathways in response to farnesol-mediated morphogenesis remains obscure. Here, we have used metabolomics to investigate the metabolic response of C. albicans upon exposure to farnesol under hyphae-inducing conditions. We have found a general up-regulation of central carbon metabolic pathways when hyphal formation was suppressed by farnesol evidenced by a considerably larger number of central carbon metabolic intermediates detected under this condition at an overall lower intracellular level. By combining the metabolic profiles from farnesol-exposed cells with previous metabolomics data for C. albicans undergoing morphogenesis, we have identified several metabolic pathways that are likely to be associated with the morphogenetic process of C. albicans, as well as metabolic pathways such as those involved in lipid metabolism that appeared to be specifically affected by farnesol. Therefore, our results provide important new insights into the metabolic role of farnesol in C. albicans metabolism.

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“…The petite mutation is reported to be lethal for Candida albicans (Alvarez & MacKinnon, 1957;Bulder, 1964a, b), but a petite-like variant with an alternative respiratory pathway has been described (Kot et al, 1976). Yamaguchi (1974Yamaguchi ( , 1975 reported that the respiratory activity of yeast cells of C . albicans declined when they were placed under germinating conditions.…”

Section: Growth Suppression By Antimycin a And Shammentioning

confidence: 99%

Phenotypic Characteristics of a Slow-growing, Nongerminating Variant of Candida albicans

Howard¹,

Zeuthen²,

Dabrowa³

1986

Microbiology

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Some of the phenotypic characteristics of a slow-growing, nongerminating variant of a commonly studied strain of Candida albicans are described. The variant arose as a chance isolate. The rate of occurrence was about 0 X 1% and the reversion rate was about 1 per 10(6) cells. The colony size was typically smaller than that of the parent and the yeast cells tended not to separate from one another so that catenulate strands of cells (pseudohyphae) were formed. Under standard conditions the generation time of the small-colony variant in liquid shake cultures was about twice that of the parental strain. Growth of the variant was suppressed by antimycin A, indicating that the small colony form was not the consequence of a defect in the cytochrome system. The colony size of the variant was not influenced by chlorobenzotriazole, which suggested that adenine metabolism was not involved in the small-colony phenotype. The pseudohyphal growth pattern was not relieved by high concentrations of utilizable carbohydrates, which means the catenulate microscopic appearance of the yeast cells was not simply an exaggeration of the normal growth pattern of isolates of C. albicans but more probably represented the growth of a cell-cycle mutant defective at the cell separation step. The cytoplasmic proteins of the variant and the parent were very similar though some unique peptides were displayed by each.

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Dimorphism in Candida Albicans. I. Morphology- Dependent Changes in Cellular Content of Macromolecules and Respiratory Activity

Cited by 21 publications

References 20 publications

Growth and Respiration Characteristics of Candida albicans

Growth and Respiration Characteristics of Candida albicans

The metabolic response ofCandida albicansto farnesol under hyphae-inducing conditions

Phenotypic Characteristics of a Slow-growing, Nongerminating Variant of Candida albicans

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