Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris

Abstract

Purpose: Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate solution, using phytotherapy is being sought. The present study attempted to evaluate the antilithiatic properties of Tribulus terrestris commonly called as "gokhru" which is often used in ayurveda to treat various urinary diseases including urolithiasis. Materials and Methods:The activity of Tribulus terrestris was investigated on nucleation and the growth of the calcium oxalate (CaOx) crystals as well as on oxalate induced cell injury of NRK 52E renal epithelial cells. Results: Tribulus terrestris extract exhibited a concentration dependent inhibition of nucleation and the growth of CaOx crystals. When NRK-52E cells were injured by exposure to oxalate for 72 h, Tribulus terrestris extract prevented the injury in a dose-dependent manner. On treatment with the different concentrations of the plant, the cell viability increased and lactate dehydrogenase release decreased in a concentration dependent manner. Conclusion: The current data suggests that Tribulus terrestris extract not only has a potential to inhibit nucleation and the growth of the CaOx crystals but also has a cytoprotective role. Our results indicate that it could be a potential candidate for phytotherapy against urolithiasis.

Cytoprotective role of the aqueous extract of Terminalia chebula on renal epithelial cells

“…The urolithiasis clinical treatment has been extensively studied with different herbs, otherwise, others studies demonstrated a potent inhibitory effect on CaOx crystal adhesion and endocytosis of folk plants, as Phyllanthus niruri, from South America, Africa in vitro and vivo models (1,2).…”

“…Calcium oxalate stones are found in two different varieties, calcium oxalate monohydrate (COM) or Whewellite, and calcium oxalate dihydrate (COD) or Weddellite. COM, the thermodynamically most stable form, is observed more frequently in clinical stones than COD and has a greater affi nity for renal tubular cells, thus responsible for the formation of stones in the kidney (2). Crystal growth and agglomeration may be due to supersaturation with respect to stone forming constituents or the Purpose: Kidney stone is one of the most prevalent diseases worldwide.…”

Evaluation of antiurolithiatic effects of Parmelia perlata against calcium oxalate calculi in hyperoxaluric rats

“…Crystal deposition is usually associated with cellular injury and caused a variety of changes in renal architecture that facilitate crystal retention. The interactions between injured tubular epithelial cells and oxalate crystals are considered to have a significant role in the development of urinary stones (Aggarwal et al, 2010;Bijarnia et al, 2008;Grover et al, 1990;Khan, 2004;Khan et al, 2000). PPE (100, 300 and 500 mg/kg), when compared with urolithiatic group, significantly restored the normal renal architecture as shown in Figure 1 D, E, and F respectively.…”

2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury

“…Media was supplemented with 1% Penicillin (100 units/mL)-Streptomycin (10,000 µg/mL) and 10% fetal bovine serum. Cells were cultured in 25 cm 2 tissue-culture treated flasks at 37º C and 5% CO 2 in humidified chambers (19).…”