Diminished adhesion of CD4+ T cells from dialysis patients to extracellular matrix and its components fibronectin and laminin

Zeltzer, Ella; Bernheim, Jacques; Korzets, Ze’ev; Rathaus, M.; Mekori, Yoseph A.; Hershkoviz, Rami

doi:10.1093/ndt/12.12.2618

Cited by 8 publications

(6 citation statements)

References 4 publications

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“…vaccination with 40 Ìg HBsAg at 0, 1, 2, and 6 months is only about 70% [10]. The immunity is characteristically compromised in uraemic, especially cellular immunity, with disruption of various T lymphocyte functions [11]. Many vaccination schemes and modifications for non-responding PCHD are proposed today.…”

Section: Discussionmentioning

confidence: 99%

Efficient Haemodialysis Improves the Response to Hepatitis B Virus Vaccination

Kovačić¹,

Šain²,

Vukman³

2002

Intervirology

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Background: As patients on chronic haemodialysis (PCHD) elicit a weaker response to vaccination with recombinant hepatitis B virus surface antigen (HBsAg), we conducted this study to see how dialysis efficacy affects response to hepatitis B virus (HBV) vaccination. Methods: Study subjects consisted of 30 PCHD. All subjects were vaccinated with 4 × 40 µg HBsAg i.m. at 0, 1, 2, and 6 months. If a subject had an HBsAg antibody (HBsAb) level <10 IU/l after vaccination, he or she received a booster dose. Subjects were divided into groups according to the level of HBsAb: non-responders (<10 IU/l), weak responders (10–100 IU/l), and good responders (>100 IU/l). Results: The group of responders had a significantly more efficient dialysis (Kt/V) than the group of non-responders (p = 0.027). This difference was not observed between groups of non-responders and weak responders. The group of good responders had a significantly better Kt/V than the group of non-responders (p = 0.012). Good responders had a significantly better Kt/V than weak responders (p = 0.019). Kt/V values showed a significantly positive correlation with the HBsAb level (r = 0.47; p = 0.006). Conclusions: The HBV vaccination reaction was weaker in PCHD with inefficient dialysis. Efficient haemodialysis significantly improves the response to vaccination with recombinant HBsAg.

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Section: Discussionmentioning

confidence: 99%

Efficient Haemodialysis Improves the Response to Hepatitis B Virus Vaccination

Kovačić¹,

Šain²,

Vukman³

2002

Intervirology

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“…We can also speculate on more specific, synergistic interactions between the clinical and cytokine variables. Low serum albumin levels have been strongly linked to impaired immune function, bacteremia, and sepsis in hemodialysis patients (Zeltzer et al , 1997; Katneni and Hedayati, 2007). In the context of impaired immunity, low levels of IL‐12 and Ang could exacerbate a predisposition to infection.…”

Section: Resultsmentioning

confidence: 99%

An integrated approach to prognosis using protein microarrays and nonparametric methods

Knickerbocker

Chen

Thadhani

et al. 2007

Molecular Systems Biology

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Over the past several years, multivariate approaches have been developed that address the problem of disease diagnosis. Here, we report an integrated approach to the problem of prognosis that uses protein microarrays to measure a focused set of molecular markers and non-parametric methods to reveal non-linear relationships among these markers, clinical variables, and patient outcome. As proof-of-concept, we applied our approach to the prediction of early mortality in patients initiating kidney dialysis. We found that molecular markers are not uniformly prognostic, but instead vary in their value depending on a combination of clinical variables. This may explain why reports in this area aiming to identify prognostic markers, without taking into account clinical variables, are either conflicting or show that markers have marginal prognostic value. Just as treatments are now being tailored to specific subsets of patients, our results show that prognosis can also benefit from a 'personalized' approach.

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“…This technique requires easily obtained, commercially available micro-flow chambers in combination with a syringe pump, greatly simplifying the equipment necessary to model blood flow and shear stress as compared to other models. 9 Another major advantage of this assay is that specific signaling cascades and the resulting activation state of individual integrins can be cleanly studied through the use of engineered CHO cells expressing human adhesion molecules of interest. Additionally, the combination of quantitative data with live cell imaging is a significant advantage of this method.…”

Section: Introductionmentioning

confidence: 99%

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Strazza¹,

Azoulay-Alfaguter²,

Peled³

et al. 2016

JoVE

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Overall, T cell adhesion is a critical component of function, contributing to the distinct processes of cellular recruitment to sites of inflammation and interaction with antigen presenting cells (APC) in the formation of immunological synapses. These two contexts of T cell adhesion differ in that T cell-APC interactions can be considered static, while T cell-blood vessel interactions are challenged by the shear stress generated by circulation itself. T cell-APC interactions are classified as static in that the two cellular partners are static relative to each other. Usually, this interaction occurs within the lymph nodes. As a T cell interacts with the blood vessel wall, the cells arrest and must resist the generated shear stress.(1,2) These differences highlight the need to better understand static adhesion and adhesion under flow conditions as two distinct regulatory processes. The regulation of T cell adhesion can be most succinctly described as controlling the affinity state of integrin molecules expressed on the cell surface, and thereby regulating the interaction of integrins with the adhesion molecule ligands expressed on the surface of the interacting cell. Our current understanding of the regulation of integrin affinity states comes from often simplistic in vitro model systems. The assay of adhesion using flow conditions described here allows for the visualization and accurate quantification of T cell-epithelial cell interactions in real time following a stimulus. An adhesion under flow assay can be applied to studies of adhesion signaling within T cells following treatment with inhibitory or stimulatory substances. Additionally, this assay can be expanded beyond T cell signaling to any adhesive leukocyte population and any integrin-adhesion molecule pair.

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Diminished adhesion of CD4+ T cells from dialysis patients to extracellular matrix and its components fibronectin and laminin

Abstract: T cells of dialysis patients exhibit abnormal adhesive activity, which may be due to an acquired cellular defect induced by uraemic milieu. CAPD patients show a greater degree of adhesion impairment, possibly due to their lower concentrations of serum albumin.

Cited by 8 publications

References 4 publications

Efficient Haemodialysis Improves the Response to Hepatitis B Virus Vaccination

Efficient Haemodialysis Improves the Response to Hepatitis B Virus Vaccination

An integrated approach to prognosis using protein microarrays and nonparametric methods

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

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