Dimethyl Sulfoxide and Related Polar Compounds Enhance Infection of Human T Cells with HIV-1in Vitro

Seki, Junichi; Ikeda, Ryuji; Hoshino, Hiroo

doi:10.1006/bbrc.1996.1576

Cited by 14 publications

(16 citation statements)

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“…Exposure to 1 to 5 mM HMBA induces HIV expression in latently infected cell lines, with most studies finding maximal induction at 5 mM (3,62,74). We reevaluated the effect of 0.2 to 25 mM HMBA in PBMCs and cell lines chosen for our studies and confirmed that 5 mM HMBA induces maximal expression without an appreciable effect on cell viability ( Fig.…”

Section: Hmba Activation Of Hiv Ltr Is Tat Independentsupporting

confidence: 56%

“…Ideally, drugs that interrupt HIV latency should be able to stimulate basal expression by Tat-independent mechanisms. Early studies differed as to the ability of HMBA to induce HIV expression in the absence of Tat (62,72). To resolve these uncertainties, we studied the effect of HMBA in cell lines stably transfected with an LTRreporter gene construct and three cell lines carrying HIV provirus with functioning and defective Tat activation pathways.…”

Section: Hmba Activation Of Hiv Ltr Is Tat Independentmentioning

confidence: 99%

“…HMBA is known to activate HIV expression in chronically infected cell lines (3,62,74) and in cell lines stably transfected with long terminal repeat (LTR)-reporter gene constructs (66,81). While HMBA is structurally related to HDAC inhibitors, it does not inhibit HDACs or induce histone hyperacetylation (56).…”

Section: The Introduction Of Highly Active Antiretroviral Therapy (Hamentioning

confidence: 99%

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Hexamethylbisacetamide Remodels the Human Immunodeficiency Virus Type 1 (HIV-1) Promoter and Induces Tat-Independent HIV-1 Expression but Blunts Cell Activation

Klichko

Archin

Kaur

et al. 2006

J Virol

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Section: Hmba Activation Of Hiv Ltr Is Tat Independentsupporting

confidence: 56%

Section: Hmba Activation Of Hiv Ltr Is Tat Independentmentioning

confidence: 99%

Section: The Introduction Of Highly Active Antiretroviral Therapy (Hamentioning

confidence: 99%

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Hexamethylbisacetamide Remodels the Human Immunodeficiency Virus Type 1 (HIV-1) Promoter and Induces Tat-Independent HIV-1 Expression but Blunts Cell Activation

Klichko

Archin

Kaur

et al. 2006

J Virol

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“…It should be emphasized that when compounds required the addition of DMSO to be solubilized in water, the concentration in the volume of DMSO used was always less than 10% with respect to water (the final concentration of DMSO in MT-4 cell incubation medium being less than 2%). Since DMSO could affect the antiviral activity of the tested drugs, 38) antiviral assays in which solutions containing equal concentrations of DMSO in water were used. Moreover, the final DMSO concentration (1/1000) was well below the concentration which affected the in vitro HIV-1 infection of T-cells.…”

Section: Methodsmentioning

confidence: 99%

Nonnucleoside HIV-1 Reverse Transcriptase Inhibitors: Part I. Synthesis and Structure-Activity Relationship of 1-Alkoxymethyl-5-alkyl-6-naphthylmethyl Uracils as HEPT Analogues

Meng

Chen

Clercq

et al. 2003

CHEMICAL & PHARMACEUTICAL BULLETIN

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1-alkoxymethyl-5-alkyl-6-naphthylmethyl uracils, which are novel 1-[(2-hydroxyethoxy)methyl]-6-(phenylthio)thymine (HEPT) analogues, were synthesized for evaluation as selective and potent nonnucleoside human immunodeficiency virus (HIV)-1 reverse transcriptase inhibitors. The anti-HIV-1 activity of these compounds was assayed in vitro using HIV-1 infected MT-4 and CEM bioassays. The EC50, CC50 and SI were recorded and calculated. The appropriate position, especially in the 1-position of the naphthyl ring, led to dramatic increases in potency, in both MT-4 and CEM cellular assays. The most important compounds in this series, 1-ethoxymethyl-5-isopropyl-6-(1-naphthylmethyl)thymine 8l (IC50=17 nM, CC50=38332 nM, SI=2229) and 1-benzyloxymethyl-5-ethyl-6-(1-naphthylmethyl)thymine 8n (IC50=17 nM, CC50=32560 nM, SI=1889) were significantly more potent than HEPT (EC50=7.0 microM, CD50=740 microM) in the anti-HIV-1 in vitro cellular assay.

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“…We also checked the effect of staurosporine, a potent inhibitor of the protein kinase C (PKC) [28], on the enhancement of albumin mRNA induced by Asc2P using Hep G2 cells. Staurosporine (50 nM) inhibited the enhancement of albumin mRNA by Asc2P, but hardly affect cellular viability by the Asc2P-free control culture (Figure 7).…”

Section: Effect Of Asc2p On Porcine Albumin and C/ebp Alpha Mrna Exprmentioning

confidence: 99%

Long-term culture of primary porcine mature hepatocytes in the medium supplemented with ascorbic acid 2-phosphate

Kumaki

Kumaki²,

Guo³

et al. 2010

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In this study, the effect of ascorbic acid 2-phosphate (Asc2P) was tested on porcine and rat mature hepatocytes in vitro. a). Asc2P increased the porcine, but not rat, albumin secretion and mRNA expression. The enhancing effect of Asc2P on porcine C/EBP alpha mRNA was observed in porcine mature hepatocytes. These data suggested that Asc2P played an important role in the regulation of porcine albumin mRNA level. b). The enhancing effect of Asc2P on ammonium metabolic activity was also observed in porcine, but not rat, mature hepatocytes. The porcine ornithine transcarbamylase (OTC) and arginase mRNAs were augmented by Asc2P, indicating that Asc2P had a direct effect on the urea cycle. c). The porcine collagen type I and type III mRNA, but not type XII mRNA, were detected as well, sugessting that Asc2P did not have the effect on the non-parenchymal hepatocytes to induce collagen type I and III mRNA expression. d). Our RT-PCR analysis demonstrated that the porcine hepatocytes expressed the sodium-ascorbate co-transporters SVCT1 and SVCT2, however, the intensities of porcine sodium-ascorbate co-transporters SVCT1 and SVCT2 bands were not changed markedly. These findings indicated that the Asc2P had no effect on SVCT1 and SVCT2 mRNA expression. e). The enhancing effect of Asc2P on porcine albumin mRNA was inhibited by staurosporine, a portein kinase inhibitor. We conclude that the enhanced albumin mRNA by Asc2P might be due to activation of tyrosine protein kinase and/or PKC and the Asc2P enhanced porcine albumin mRNA mainly at the transcriptional step

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Dimethyl Sulfoxide and Related Polar Compounds Enhance Infection of Human T Cells with HIV-1in Vitro

Cited by 14 publications

References 0 publications

Hexamethylbisacetamide Remodels the Human Immunodeficiency Virus Type 1 (HIV-1) Promoter and Induces Tat-Independent HIV-1 Expression but Blunts Cell Activation

Hexamethylbisacetamide Remodels the Human Immunodeficiency Virus Type 1 (HIV-1) Promoter and Induces Tat-Independent HIV-1 Expression but Blunts Cell Activation

Nonnucleoside HIV-1 Reverse Transcriptase Inhibitors: Part I. Synthesis and Structure-Activity Relationship of 1-Alkoxymethyl-5-alkyl-6-naphthylmethyl Uracils as HEPT Analogues

Long-term culture of primary porcine mature hepatocytes in the medium supplemented with ascorbic acid 2-phosphate

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