Dimensional comparison between amplitude-modulation atomic force microscopy and scanning ion conductance microscopy of biological samples

Kim, Joonhui; Choi, Myung-Hoon; Jung, Goo-Eun; Ferhan, Abdul Rahim; Cho, Nam‐Joon; Cho, Sang-Joon

doi:10.7567/jjap.55.08nb18

Cited by 5 publications

(5 citation statements)

References 30 publications

(35 reference statements)

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“…In previous studies, SICM has consistently reported higher apparent heights observed relative to AFM measurements, presumably a result of tip−sample forces applied in tapping mode AFM, which resulted in height information measured by AFM being underestimated in the range of 30−50%. 9,22 For measurements recorded here with SICM, BP-SICM PF was not as accurate as BP-SICM NF , a result of the differences in D ps for different feedback regimes.…”

Section: ■ Results and Discussionmentioning

confidence: 91%

Biphasic-Scanning Ion Conductance Microscopy

Choi

Baker

2018

Anal. Chem.

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A concentration gradient driven imaging mechanism is described for scanning ion conductance microscopy (SICM). Two different solution phases, one filling a double-barrel pipet and one in the bath, are used to afford probe control and imaging under nonstandard SICM conditions. Under these conditions, solutions with no added electrolyte can be utilized as the bath solution. Further, both positive and negative feedback modes are exhibited as the probe approaches the surface. We term this method biphasic-SICM (BP-SICM). Technical details of implementing BP-SICM and operational principles are described herein.

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Section: ■ Results and Discussionmentioning

confidence: 91%

Biphasic-Scanning Ion Conductance Microscopy

Choi

Baker

2018

Anal. Chem.

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“…The softness (spring constant: 0.1 N/m) of the cantilever (BL-AC40TS, Olympus) and small dimension of the cantilever (length 38 μm and width 16 μm) and tip (ca. 10 nm radius) used here provide sufficient sensitivity for imaging in hydrated conditions with high topographical resolution. ,− Topography data was analyzed by XEI software (Park Systems) , and Gwyddion . Tip-deconvolution analysis of AFM topography was conducted via ProfilmOnline (KLA) (for details of tip-deconvolution, see the Results and Discussion section).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Ligand Adsorption at Individual Gold Nanocubes

et al. 2021

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Cetyltrimethylammonium bromide (CTAB) is a widely used surfactant that aids the aqueous synthesis of colloidal nanoparticles. However, the presence of residual CTAB on nanoparticle surfaces can significantly impact nanoparticle applications, such as catalysis and sensing, under hydrated conditions. As such, consideration of the presence and quantity of CTAB on nanoparticle surfaces under hydrated conditions is of significance. Herein, as part of an integrated material characterization framework, we demonstrate the feasibility of in situ atomic force microscopy (AFM) to detect CTAB on the surface of Au nanocubes (Au NCs) under hydrated conditions, which enabled superior characterization compared to conventional spectroscopic methods. In situ force–distance (FD) spectroscopy and Kelvin probe force microscopy (KPFM) measurements support additional characterization of adsorbed CTAB, while correlative in situ AFM and scanning electron microscopy (SEM) measurements were used to evaluate sequential steps of CTAB removal from Au NCs across hydrated and dehydrated environments, respectively. Notably, a substantial quantity of CTAB remained on the Au NC surface after methanol washing, which was detected in AFM measurements but was not detected in infrared spectroscopy measurements. Subsequent electrochemical cleaning was found to be critically important to remove CTAB from the Au NC surface. Correlative measurements were also performed on individual nanoparticles, which further validate the method described here as a powerful tool to determine the extent and degree of CTAB removal from nanoparticle surfaces. This AFM-based method is broadly applicable to characterize the presence and removal of ligands from nanomaterial surfaces under hydrated conditions.

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“…After minimally invasive puncture of cells, a small amount of cytoplasmic sol can be collected from different locations in a single cell under the guidance of SICM imaging. The results show that the expression of mRNA is related to the location of cells 53 . Zhou et al combined with fluorescence imaging, the morphology of primary cilia of different cell types can also be observed and different types of cilia can be distinguished 45 .…”

Section: Application Of Sicm In Biomedical Researchmentioning

confidence: 99%

“…The results show that the expression of mRNA is related to the location of cells. 53 Zhou et al combined with fluorescence imaging, the morphology of primary cilia of different cell types can also be observed and different types of cilia can be distinguished. 45 In addition, the nanoinjection technology proposed by Simonis et al that injects fluorescent molecules into individual cells through SICM provides a direction for the development of building more portable and more cost-efficient system for the injection of single cells.…”

Section: High-precision Imaging Of Cellular Structuresmentioning

confidence: 99%

Applications of scanning ion conductance microscope in biomedical fields

Gong

Fan

Huang

et al. 2023

MedComm – Biomaterials and Applications

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Scanning ion conductance microscopy (SICM) is a scanning probe technique to reflect the surface morphology of the sample with the probe's motion trajectory through measuring the current between the probe and the surface of the sample. Since the surface of the sample can be scanned noncontact and the morphological characteristics of the living cell can be obtained under physiological conditions, SICM is particularly important in the field of biomedicine. SICM has strong potentials in various applications, such as the real-time and high-resolution imaging of living cells, monitoring the volume and movements of living cells. In addition, SICM combines with various technologies such as patch clamp and nanopipette to study the physical properties of cells. Furthermore, using SICM to observe cell and tissue structure provides more directions for studying the mechanism of disease. This review briefly introduces the principles and imaging modes of SICM and outlines the recent applications and development of SICM in biomedical research. We also introduce the limitations of the existing SICM technology and make a perspective on its future development.

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Dimensional comparison between amplitude-modulation atomic force microscopy and scanning ion conductance microscopy of biological samples

Cited by 5 publications

References 30 publications

Biphasic-Scanning Ion Conductance Microscopy

Biphasic-Scanning Ion Conductance Microscopy

Characterization of Ligand Adsorption at Individual Gold Nanocubes

Applications of scanning ion conductance microscope in biomedical fields

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