Dilute phosphoric acid pretreatment of wheat bran for enzymatic hydrolysis and subsequent ethanol production by edible fungi Neurospora intermedia

Nair, Ramkumar B.; Lundin, Magnus; Brandberg, Tomas; Lennartsson, Patrik R.; Taherzadeh, Mohammad J.

doi:10.1016/j.indcrop.2015.02.038

Cited by 61 publications

(36 citation statements)

References 23 publications

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“…The data for the simulation was obtained from the ethanol plant, and the improvement data such as thin stillage/stillage to ethanol and lignocelluloses to ethanol was based on the laboratory data from Ferreira et al and Nair et al [28,29]. The process simulation was carried out using Aspen Plus ® (v.8.4) (Aspentech: Burlington, MA, USA).…”

Section: Process Descriptionmentioning

confidence: 99%

“…The wheat bran had 88% TS, while the pretreatment method required a solid loading of 15% TS; hence, water was added to dilute the solids concentration. A chemical pretreatment method was employed using 1.5% phosphoric acid for a residence time of 10 min at 190˝C [29]. It should be noted that the choice of the pretreating acid was dictated by the properties of the feed, as the common sulfuric acid could not be used.…”

Section: Integration Of Lignocelluloses (Scenario C)mentioning

confidence: 99%

“…Carbon dioxide produced during the fermentation was sold along with the carbon dioxide produced from the grains to ethanol process, as a byproduct. All the data for this simulation were based on the laboratory data from a previous work [29]. After the pretreatment process, the simultaneous saccharification and fermentation (SSF) process was employed to convert the pretreated lignocellulose into ethanol.…”

Section: Integration Of Lignocelluloses (Scenario C)mentioning

confidence: 99%

Section: Integration Of Lignocelluloses (Scenario C)mentioning

confidence: 99%

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Techno-Economic Analysis of Integrating First and Second-Generation Ethanol Production Using Filamentous Fungi: An Industrial Case Study

2016

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Abstract:The 2nd generation plants producing ethanol from lignocelluloses demand risky and high investment costs. This paper presents the energy-and economical evaluations for integrating lignocellulose in current 1st generation dry mill ethanol processes, using filamentous fungi. Dry mills use grains and have mills, liquefactions, saccharifications, fermentation, and distillation to produce ethanol, while their stillage passes centrifugation, and evaporation to recycle the water and dry the cake and evaporated syrup into animal feed. In this work, a bioreactor was considered to cultivate fungi on the stillage either before or after the centrifugation step together with pretreated lignocellulosic wheat bran. The results showed that the integrated 1st and 2nd generation ethanol process requires a capital investment of 77 million USD, which could yield NPV of 162 million USD after 20 years. Compared to the fungal cultivation on thin stillage modified 1st generation process, the integrated process resulted in 53 million USD higher NPV. The energy analysis showed that the thin stillage modified 1st generation process could reduce the overall energy consumption by 2.5% and increase the ethanol production by 4%. Such modifications in the 1st generation processes and integration concepts could be interesting for the ethanol industries, as integrating lignocelluloses to their existing setup requires less capital investment.

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Section: Process Descriptionmentioning

confidence: 99%

Section: Integration Of Lignocelluloses (Scenario C)mentioning

confidence: 99%

Section: Integration Of Lignocelluloses (Scenario C)mentioning

confidence: 99%

Section: Integration Of Lignocelluloses (Scenario C)mentioning

confidence: 99%

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Techno-Economic Analysis of Integrating First and Second-Generation Ethanol Production Using Filamentous Fungi: An Industrial Case Study

2016

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“…The decreasing pattern of sugars indicates that fungi consumed the released sugars during the prolonged incubation, as described previously [ El-Gammal et al, 1998]. Until now, fungal methods applied on SB and WB degradation had not shown an additional improvement of hydrolyzed sugar recovery, including the combination of them with physicochemical methods [Farani de Souza et al, 2006;Fonseca-Maldonado et al, 2014;Jiang and Guo, 2016;Kuhar et al, 2008;Lima et al, 2015;Nair et al, 2015;Papinutti et al, 2003]. Even more surprising was that the amount of glucose and xylose released employing the recombinant strains was higher than previously reported using only physicochemical methods [Favaro et al, 2013;Hell et al, 2015;Liu et al, 2016;Moretti et al, 2014].…”

Section: Discussionmentioning

confidence: 56%

Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus <b><i>Phanerochaete chrysosporium</i></b> Overexpressing Laccases and Peroxidases

et al. 2018

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Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production, Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain. Sugar release on lignocellulose was 2- to 6-fold higher by recombinant fungi as compared with the control. Wheat bran ostensibly stimulated the production of ligninolytic enzymes. The highest peroxidase activity from the recombinant strains was 2.6-fold higher, whereas the increase in laccase activity was 4-fold higher in comparison to the control. The improvement of lignin degradation was directly proportional to the highest peroxidase and laccase activity. Because various phenolic compounds released during lignocellulose degradation have proven to be toxic to cells and to inhibit enzyme activity, a significant reduction (over 40%) of the total phenolic content in the samples treated with recombinant strains was observed. To our knowledge, this is the first report that engineering P. chrysosporium enhances biodegradation of lignocellulosic biomass.

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Optimizing dilute phosphoric acid pretreatment of wheat straw in the laboratory and in a demonstration plant for ethanol and edible fungal biomass production using Neurospora intermedia

Nair

Lundin

Lennartsson

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUND: We report a method that uses dilute phosphoric acid for wheat straw pretreatment and subsequent ethanol and fungal biomass production with the edible fungus Neurospora intermedia. Dilute phosphoric acid pretreatment of wheat straw was optimized at a laboratory scale, and the results were validated in a biorefinery demonstration plant for the first time. The various conditions for the dilute acid pretreatment include such factors as phosphoric acid concentrations (0.5-3.0% w/v), temperature (150-210°C), and reaction time (5-20 min).RESULTS: The optimal pretreatment conditions were determined as an acid concentration of 1.75% (w/v) at a temperature of 190°C for 15 min, based on the maximum enzymatic digestibility with the minimum inhibitor release. The efficiency of enzymatic polysaccharide hydrolysis was 36% for untreated straw and 86% for straw pretreated with dilute phosphoric acid. Scale up of the pretreatment at a biorefinery demonstration plant improved the process, with the subsequent efficiency of polysaccharide hydrolysis being 95% of the theoretical maximum. Ethanol fermentation of enzymatically hydrolyzed wheat straw using N.intermedia showed an improvement in the ethanol yield from 29% (with untreated straw) to 94% (with dilute phosphoric acid pretreated straw) of the theoretical maximum.CONCLUSION: This study opens up an alternative strategy for the efficient use of wheat straw for the production of ethanol and edible fungal biomass in existing wheat-to-ethanol plants.

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Dilute phosphoric acid pretreatment of wheat bran for enzymatic hydrolysis and subsequent ethanol production by edible fungi Neurospora intermedia

Cited by 61 publications

References 23 publications

Techno-Economic Analysis of Integrating First and Second-Generation Ethanol Production Using Filamentous Fungi: An Industrial Case Study

Techno-Economic Analysis of Integrating First and Second-Generation Ethanol Production Using Filamentous Fungi: An Industrial Case Study

Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus <b><i>Phanerochaete chrysosporium</i></b> Overexpressing Laccases and Peroxidases

Optimizing dilute phosphoric acid pretreatment of wheat straw in the laboratory and in a demonstration plant for ethanol and edible fungal biomass production using Neurospora intermedia

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