Dilute (d) coat colour mutation of DBA/2J mice is associated with the site of integration of an ecotropic MuLV genome

Abstract

The single endogenous DBA/2J ectropic provirus segregated concordantly with the dilute (d) coat colour mutation on chromosome 9 in 53/53 DBA/2J-derived recombinant inbred mouse strains and all seven inbred and mutant strains tested that carry the d allele. Analysis of DNA from a spontaneous DBA/2J d revertant (d+2J) showed that these mice lack ecotropic-specific MuLV DNA sequences and suggested that the dilute mutation resulted from integration of an ecotropic provirus into the mouse genome.

The angiotensinogen gene is located on mouse chromosome 8

“…When the CXB recombinant inbred set was used to specifically examine markers on chromosome 8, the strain distribution pattern of the ecotropic murine leukaemia virus Emv-2 [ 18,191 showed only one mismatch to that determined for angioten- 3B). Finally, the segregation of salivary gland and testicular angiotensinogen expression phenotypes into inbred mouse strains [3] was not concordant with known chromosome 8 retroviral insertions Emv-2 [18], A&v-21 [21], Xmv-12 or Xmv-26 [16].…”

“…Finally, the segregation of salivary gland and testicular angiotensinogen expression phenotypes into inbred mouse strains [3] was not concordant with known chromosome 8 retroviral insertions Emv-2 [18], A&v-21 [21], Xmv-12 or Xmv-26 [16]. We conclude that the nature of the &acting genetic lesion affecting tissue-specific expression of the angiotensinogen gene will be best defined using molecular cloning techniques.…”

Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity

“…A total of 205 N 2 mice were used to map the DAPK2 locus (see text for details). DNA isolation, restriction enzyme digestion, agarose gel electrophoresis, Southern blot transfer and hybridization were performed essentially as described (Jenkins et al, 1982). All blots were prepared with Hybond-N + nylon membrane (Amersham).…”

Proviral integrations at the Evi5 locus disrupt a novel 90 kDa protein with homology to the Tre2 oncogene and cell-cycle regulatory proteins

“…A total of 205 N 2 mice were used to map the Evi5 and Evi5-related loci. DNA isolation, restriction enzyme digestion, agarose gel electrophoresis, Southern blot transfer and hybridization were performed essentially as described (Jenkins et al, 1982). All blots were prepared with Hybond-N + nylon membrane (Amersham).…”