We have recently identified a c&acting genetic lesion affecting angiotensinogen gene expression in testis and salivary gland. Accordingly, the an~otensinogen gene was assigned to mouse chromosome 8 by screening a series of hybrid cell lines for retention of mouSe angiotensinogen sequences by genomic Southern analysis. In AKXD recombinant inbred mice, the angiotensinogen gene is 2.4+ 1.8 centiMorgan from Rn7S-&a 7S RNA gene located on chromosome 8 (Taylor, B.A., personal communication). However, the segregation of salivary and testicular angiotensinogen expression phenotypes into inbred mouse strains was not concordant with the known chromosome 8 proviruses Emv-2. Mtv-21, Xmv-I2or Xmv-26.