Dilute acid pretreatment and enzymatic saccharification of sugarcane tops for bioethanol production

Sindhu, Raveendran; Kuttiraja, Mathiyazhakan; Binod, Parameswaran; Janu, Kanakambaran Usha; Sukumaran, Rajeev K.; Pandey, Ashok

doi:10.1016/j.biortech.2011.09.066

Cited by 180 publications

(62 citation statements)

References 30 publications

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“…The porous fiber structures facilitated the enzyme accessibility, which enhanced the enzymatic efficiency. Sindhu et al (2011) also observed the obvious changes on fiber surface of biomass after pretreatment for bioethanol production. …”

Section: Characterization Of Wood Pulp Waste and Pretreated Substratementioning

confidence: 84%

Sodium Hydrogen Sulfite Pretreatment of Wood Pulp Waste for Enhancement of Enzymatic Efficiency

Liu¹,

Wang²,

Yang³

et al. 2014

BioResources

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aWood pulp waste is a type of industrial waste that has an enormous potential for fermentable sugar production. Efficient pretreatment is the key for enhancing the enzymatic efficiency. In this paper, sodium hydrogen sulfite pretreatment was performed on wood pulp waste to improve enzyme performance through sulfonation and hydrolysis reactions. Results showed that the enzymatic efficiency was greatly enhanced from 5.12 to 41.6% in terms of reducing sugar yield (RSY) under the optimum conditions: 6% NaHSO3, 2500 P-factor, 3/1 liquid to solid ratio, and cellulase charge of 35 FPU/g substrate. Mass balance showed that approximately 73% of glucose was recovered. The mechanism of sulfonation and hydrolysis of sodium hydrogen sulfite pretreatment were confirmed by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (X-RD), and scanning electron microscopy (SEM).

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Section: Characterization Of Wood Pulp Waste and Pretreated Substratementioning

confidence: 84%

Sodium Hydrogen Sulfite Pretreatment of Wood Pulp Waste for Enhancement of Enzymatic Efficiency

Liu¹,

Wang²,

Yang³

et al. 2014

BioResources

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“…Pre-treatment is usually required to deconstruct the tough complex structures in lignocellulosic biomass prior to hydrolysis. Acid and alkali pre-treatment technologies have been used on lignocellulosic biomass (Krishnan et al 2010;Sindhu et al 2011); most thermal pre-treatment requires high temperatures above 100 °C (Nitsos et al 2013;Zheng and Rehmann 2014). However, fungal cellulases available for saccharification have optimum activities at 50 °C.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Kazeem¹,

Shah²,

Baharuddin³

et al. 2016

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Effects of nutritional and physicochemical factors were investigated for cellulase production by the newly isolated thermophilic strain Bacillus licheniformis 2D55 (Accession No. KT799651). The optimum cellulase production in shake flask fermentation was attained at 60 °C, pH 3.5, 180 rpm, and in a medium containing untreated sugarcane bagasse and pretreated rice husk at 7% (w/v), urea, 1 g/L, peptone, 11.0 g/L, Mg(SO4)2, 0.40 g/L, CaCl2, 0.03 g/L, Tween 80, 0.2% (w/v), and 3% inoculum. The highest carboxymethyl cellulase (CMCase), filtre paperase (FPase), and β-glucosidase produced under the optimized conditions were 29.4 U/mL, 12.9 U/mL, and 0.06 U/mL, respectively, after 18 h of fermentation. Optimization of the parameters increased the CMCase, FPase, and β-glucosidase activities by 77.4-fold, 44.5-fold, and 10-fold, respectively. The crude enzyme was highly active and stable over broad temperature (50 to 80 °C) and pH (3.5 to 10.0) ranges with optimum temperature at 65 °C and 80 ºC for CMCase and FPase, respectively. The optimum pH for CMCase and FPase was 7.5 and 6.0, respectively. Saccharification of sugar cane bagasse and rice husk by crude cellulase resulted in perspective yields of 0.348 and 0.301 g g -1 dry substrate of reducing sugars. These results suggest prospects of thermostable cellulase from B. licheniformis 2D55 in application for bio-sugar production and other industrial bioprocess applications involving high temperatures.

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“…The samples were scanned in a range of 2θ=10°-30° with a step size of 0.02° at 500 kV, 30 mA and radiation at Cu Kα (λ=1.54 Å). Crystallinity was calculated according to the following equations for crystallinity index below (18). …”

Section: X-ray Diffraction Analysismentioning

confidence: 99%

Effect of Combination of Liquid Hot Water System and Hydrogen Peroxide Pretreatment on Enzymatic Saccharification of Corn Cob

Upajak¹

2018

GEOMATE

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Alkaline hydrogen peroxide pretreatment is an effectively enhance the increasing enzymatic digestibility of lignocellulosic biomass for conversion to fuels and chemicals in the biorefinery processes. In this study, effects of H 2 O 2 on monomeric sugar in the liquid fraction during hydrogen peroxide pretreatment and sugar after enzymatic hydrolysis from corncobs were studied under varying reaction conditions. The temperature (30-120 o C) and H 2 O 2 concentration (2.5-10%) efficiently promoted sugar yield in the piqued fraction and improved enzymatic hydrolysis of pretreated solids. The optimal condition for H 2 O 2 pretreatment of corncob (H 2 O 2 concentration of 5% using 60 o C for 2 h) increased hemicellulose solubilization into the aqueous phase, resulting into the maximized pentose yield of 61.88% (xylose + arabinose) in the aqueous phase. H 2 O 2 pretreatment under the optimal conditions at 60 o C for 2 h, leading to the enhance glucose yield from enzymatic hydrolysis of the pretreated biomass using 10 FPU/g CelluclastTM (85.66 %) and small amount of formation of inhibitory by-products. Combined with glucose in the aqueous phase, this resulted in the maxima 95.61% glucose recovery from the native corncob. This was related to changes in crystallinity and surface area of the pretreated biomass. Scanning electron microscopy (SEM) showed disruption of the intact biomass structure resulting increasing enzyme's accessibility to the cellulose microfibers which showed higher crystallinity index compared to the native biomass as shown by X-ray diffraction with a marked increase in surface area as revealed by BET measurement. The results provided efficiency of H2O2 pretreatment on increasing sugar recovery and an efficient approach for its processing in biorefinery industry.

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Dilute acid pretreatment and enzymatic saccharification of sugarcane tops for bioethanol production

Cited by 180 publications

References 30 publications

Sodium Hydrogen Sulfite Pretreatment of Wood Pulp Waste for Enhancement of Enzymatic Efficiency

Sodium Hydrogen Sulfite Pretreatment of Wood Pulp Waste for Enhancement of Enzymatic Efficiency

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Effect of Combination of Liquid Hot Water System and Hydrogen Peroxide Pretreatment on Enzymatic Saccharification of Corn Cob

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