Dihydroxyacetone: An intermediate in the assimilation of methanol by yeasts?

Dijken, Johannes P. van; Harder, W.; Beardsmore, A.J.; Quayle, J. R.

doi:10.1111/j.1574-6968.1978.tb08674.x

Cited by 41 publications

(13 citation statements)

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“…Carbon assimilation occurs probably from formaldehyde 2 ) but the exact mechanism in yeast is still unknown. 7 ) The enzymes of methanol metabolism have been studied in yeasts especially the alcohol oxidases from Kloeckera,8.9) Candida 10 ,1l) 2280 R. COUDERC and J. BARATTI and Hansenula polymorpha 12 ,13) but also formaldehyde and formate dehydrogenasesy, 14,17) The present work is a study of methanol oxidation by the yeast, P. pastoris IFP 206.…”

mentioning

confidence: 99%

Oxidation of Methanol by the Yeast,Pichia pastoris. Purification and Properties of the Alcohol Oxidase

Couderc¹,

Baratti²

1980

Agricultural and Biological Chemistry

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mentioning

confidence: 99%

Oxidation of Methanol by the Yeast,Pichia pastoris. Purification and Properties of the Alcohol Oxidase

Couderc¹,

Baratti²

1980

Agricultural and Biological Chemistry

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“…catalyzed by a transketolase induced by metha nol. This reaction mechanism of the formalde hyde-fixing enzyme of the yeast is completely different from that of bacterial 3-hexulose phosphate synthase, which is known to be a kind of aldolase.6,16) Dihydroxyacetone form ed by this reaction may be further metabolized as described by van Dijken et al 8) Further purification and the characterization of the enzyme are being performed.…”

mentioning

confidence: 99%

Xylulose 5-phosphate-dependent fixation of formaldehyde in a methanol-utilizing yeast, Kloeckera sp. No. 2201.

Kato

Nishizawa

Sakazawa

et al. 1979

Agricultural and Biological Chemistry

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“…GSH is removed by S-formyl glutathione hydrolase (FGH) and formate is converted to CO 2 by formate dehydrogenase (FDH). Oxidation of formaldehyde generates 2 molecules of NADH that are used for ATP generation in mitochondria [30].…”

Section: Resultsmentioning

confidence: 99%

Extension of Yeast Chronological Lifespan by Methylamine

et al. 2012

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BackgroundChronological aging of yeast cells is commonly used as a model for aging of human post-mitotic cells. The yeast Saccharomyces cerevisiae grown on glucose in the presence of ammonium sulphate is mainly used in yeast aging research. We have analyzed chronological aging of the yeast Hansenula polymorpha grown at conditions that require primary peroxisome metabolism for growth.Methodology/Principal FindingsThe chronological lifespan of H. polymorpha is strongly enhanced when cells are grown on methanol or ethanol, metabolized by peroxisome enzymes, relative to growth on glucose that does not require peroxisomes. The short lifespan of H. polymorpha on glucose is mainly due to medium acidification, whereas most likely ROS do not play an important role. Growth of cells on methanol/methylamine instead of methanol/ammonium sulphate resulted in further lifespan enhancement. This was unrelated to medium acidification. We show that oxidation of methylamine by peroxisomal amine oxidase at carbon starvation conditions is responsible for lifespan extension. The methylamine oxidation product formaldehyde is further oxidized resulting in NADH generation, which contributes to increased ATP generation and reduction of ROS levels in the stationary phase.Conclusion/SignificanceWe conclude that primary peroxisome metabolism enhanced chronological lifespan of H. polymorpha. Moreover, the possibility to generate NADH at carbon starvation conditions by an organic nitrogen source supports further extension of the lifespan of the cell. Consequently, the interpretation of CLS analyses in yeast should include possible effects on the energy status of the cell.

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Dihydroxyacetone: An intermediate in the assimilation of methanol by yeasts?

Cited by 41 publications

References 20 publications

Oxidation of Methanol by the Yeast,Pichia pastoris. Purification and Properties of the Alcohol Oxidase

Oxidation of Methanol by the Yeast,Pichia pastoris. Purification and Properties of the Alcohol Oxidase

Xylulose 5-phosphate-dependent fixation of formaldehyde in a methanol-utilizing yeast, Kloeckera sp. No. 2201.

Extension of Yeast Chronological Lifespan by Methylamine

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