Dihydromyricetin protects endothelial cells from hydrogen peroxide-induced oxidative stress damage by regulating mitochondrial pathways

Hou, Xiao-Long; Tong, Qingyi; Wang, Wenqing; Xiong, Wei; Shi, Chunyu; Fang, Jianguo

doi:10.1016/j.lfs.2015.03.007

Cited by 104 publications

(81 citation statements)

References 39 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, development of drugs or nutrients that improve mitochondrial function could be beneficial in preventing Dex-induced muscle atrophy. Ampelopsis grossedentata, a medicinal and edible plant growing in mountainous areas of southern China, has been consumed as a health tea and traditional medicine for hundreds of years [15]. Dihydromyricetin (DM) is the main flavonoid component of A. grossedentata, and the DM contents can be greater than 30% in vine tea made from its tender stems and leaves [16].…”

Section: Introductionmentioning

confidence: 99%

“…Ampelopsis grossedentata, a medicinal and edible plant growing in mountainous areas of southern China, has been consumed as a health tea and traditional medicine for hundreds of years [15]. Dihydromyricetin (DM) is the main flavonoid component of A. grossedentata, and the DM contents can be greater than 30% in vine tea made from its tender stems and leaves [16]. DM displays a broad range of biological and pharmacological activities such as antioxidant, anti-inflammatory, anti-tumor, neuroprotective and hepatoprotective effects [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dihydromyricetin Attenuates Dexamethasone-Induced Muscle Atrophy by Improving Mitochondrial Function via the PGC-1α Pathway

Huang¹,

Chen²,

Ren³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Skeletal muscle atrophy is an important health issue and can impose tremendous economic burdens on healthcare systems. Glucocorticoids (GCs) are well-known factors that result in muscle atrophy observed in numerous pathological conditions. Therefore, the development of effective and safe therapeutic strategies for GC-induced muscle atrophy has significant clinical implications. Some natural compounds have been shown to effectively prevent muscle atrophy under several wasting conditions. Dihydromyricetin (DM), the most abundant flavonoid in Ampelopsis grossedentata, has a broad range of health benefits, but its effects on muscle atrophy are unclear. The purpose of this study was to evaluate the effects and underlying mechanisms of DM on muscle atrophy induced by the synthetic GC dexamethasone (Dex). Methods: The effects of DM on Dex-induced muscle atrophy were assessed in Sprague-Dawley rats and L6 myotubes. Muscle mass and myofiber cross-sectional areas were analyzed in gastrocnemius muscles. Muscle function was evaluated by a grip strength test. Myosin heavy chain (MHC) content and myotube diameter were measured in myotubes. Mitochondrial morphology was observed by transmission electron microscopy and confocal laser scanning microscopy. Mitochondrial DNA (mtDNA) was quantified by real-time PCR. Mitochondrial respiratory chain complex activities were examined using the MitoProfile Rapid Microplate Assay Kit, and mitochondrial membrane potential was assessed by JC-1 staining. Protein levels of mitochondrial biogenesis and dynamics markers were detected by western blotting. Myotubes were transfected with siRNAs targeting peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), mitochondrial transcription factor A (TFAM) and mitofusin-2 (mfn2) to determine the underlying mechanisms. Results: In vivo, DM preserved muscles from weight and average fiber cross-sectional area losses and improved grip strength. In vitro, DM prevented the decrease in MHC content and myotube diameter. Moreover, DM stimulated mitochondrial biogenesis and promoted mitochondrial fusion, rescued the reduced mtDNA content, improved mitochondrial morphology, prevented the collapse in mitochondrial membrane potential and enhanced mitochondrial respiratory chain complex activities; these changes restored mitochondrial function and improved protein metabolism, contributing to the prevention of Dex-induced muscle atrophy. Furthermore, the protective effects of DM on mitochondrial function and muscle atrophy were alleviated by PGC-1α siRNA, TFAM siRNA and mfn2 siRNA transfection in vitro. Conclusion: DM attenuated Dex-induced muscle atrophy by reversing mitochondrial dysfunction, which was partially mediated by the PGC-1α/TFAM and PGC-1α/mfn2 signaling pathways. Our findings may open new avenues for identifying natural compounds that improve mitochondrial function as promising candidates for the management of muscle atrophy.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dihydromyricetin Attenuates Dexamethasone-Induced Muscle Atrophy by Improving Mitochondrial Function via the PGC-1α Pathway

Huang¹,

Chen²,

Ren³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…[8], and has attracted increasing attention owing to its beneficial effect on cells. It has been recently reported that DHM can protect endothelial cells from H2O2-induced oxidative damage via mitochondrial pathways [9], and exert antioxidant action in soybean oil and cooked ground beef model systems [10]. Additionally, the parent plant A. grossedentata has been consumed as a tea (Rattan Tea, Vine Tea, Tengcha) with health benefits in South China [11,12].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Dihydromyricetin against •OH-Induced Mesenchymal Stem Cells Damage and Mechanistic Chemistry

Liu

Lin

et al. 2016

Molecules

View full text Add to dashboard Cite

Abstract:As a natural flavonoid in Ampelopsis grossedentata, dihydromyricetin (DHM, 2R,3R-3,5,7,3 1 ,4 1 ,5 1 -hexahydroxy-2,3-dihydroflavonol) was observed to increase the viability of ‚OH-treated mesenchymal stem cells using a MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl] assay and flow cytometry analysis. This protective effect indicates DHM may be a beneficial agent for cell transplantation therapy. Mechanistic chemistry studies indicated that compared with myricetin, DHM was less effective at ABTS + ‚ (2,2 1 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical) scavenging and reducing Cu 2+ , and had higher ‚O 2´a nd DPPH‚ (1,1-diphenyl-2-picrylhydrazyl radical) scavenging activities. Additionally, DHM could also chelate Fe 2+ to give an absorption maximum at 589 nm. Hence, such protective effect of DHM may arise from its antioxidant activities which are thought to occur via direct radical-scavenging and Fe 2+ -chelation. Direct radical-scavenging involves an electron transfer (ET) pathway. The hydrogenation of the 2,3-double bond is hypothesized to reduce the ET process by blocking the formation of a larger π-π conjugative system. The glycosidation of the 3-OH in myricitrin is assumed to sterically hinder atom transfer in the ‚O 2´a nd DPPH‚ radical-scavenging processes. In DHM, the Fe 2+ -chelating effect can actually be attributed to the 5,3 1 ,4 1 ,5 1 -OH and 4-C=O groups, and the 3-OH group itself can neither scavenge radicals nor chelate metal.

show abstract

“…Recently, Prasad et al (2016) demonstrated the neurorestorative potential of eugenol in terms of its ability to normal liver cells, which supports the possibility of DHM serving as a therapeutic antitumoral candidate (Zhang et al, 2014). Hou et al (2015) indicates that DHM can protect endothelial cells from oxidative stress, increase production of ND, inhibit production of RDS, and enhance cellular antioxidant defense capabilities, therefore protecting endothelial cells from damaging effects of oxidative stress damage by regulating mitochondrial pathways. Dther study indicates that DHM improves glucose and lipid metabolism and exerts anti-inflammatory effects in fatty liver disease (Chen et al, 2015).…”

Section: Chromatography Analysismentioning

confidence: 72%

Elaboration and characterization of Japanese Raisin Tree (Hovenia dulcis Thumb.) pseudofruits fermented alcoholic beverage

et al. 2017

View full text Add to dashboard Cite

Dihydromyricetin protects endothelial cells from hydrogen peroxide-induced oxidative stress damage by regulating mitochondrial pathways

Cited by 104 publications

References 39 publications

Dihydromyricetin Attenuates Dexamethasone-Induced Muscle Atrophy by Improving Mitochondrial Function via the PGC-1α Pathway

Dihydromyricetin Attenuates Dexamethasone-Induced Muscle Atrophy by Improving Mitochondrial Function via the PGC-1α Pathway

Protective Effects of Dihydromyricetin against •OH-Induced Mesenchymal Stem Cells Damage and Mechanistic Chemistry

Elaboration and characterization of Japanese Raisin Tree (Hovenia dulcis Thumb.) pseudofruits fermented alcoholic beverage

Contact Info

Product

Resources

About