Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells

Lǚ, Yingying; Chen, Tongsheng; Qu, Junle; Pan, Wenliang; Sun, Lei; Wei, Xunbin

doi:10.1186/1423-0127-16-16

Cited by 116 publications

(110 citation statements)

References 36 publications

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“…These results were in agreement with previous studies that showed that SLAK exerted an anti-inflammatory effect in J774A.1 macrophages through the inhibition of NO and PGE 2 production as well iNOS and COX-2 expression (Emami et al, 2010). Moreover, the overall impact of these changes also reflect results of previous studies that demonstrated that various individual sesquiterpene compounds from Artemisia exert anti-inflammatory effects that may be effective in treating some types of experimental inflammation/autoimmune conditions (Choi et al, 2011;Ho et al, 2014;Kim et al, 2002;Lu et al, 2009;Omer et al, 2007;Sun et al, 2010;Yang et al, 2012).…”

Section: Discussionsupporting

confidence: 82%

“…Different classes of chemicals, e.g., coumarins, terpenes, sesquiterpenes, flavonoids, aromatics, dipeptides, phenolics, esterols, germacranolides, secoguaianolides, and several polysaccharides have been isolated from Artemisia species (Valless et al, 2011). In recent years, increasing evidence has shown that sesquiterpene lactones are one of the constituents that impart a wide-range of pharmacological effects, including anti-cancer and immunomodulatory actions (Choi et al, 2011;Kim et al, 2002;Lu et al, 2009;Yang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

Rabe¹,

Iranshahi

Rastin³

et al. 2014

Journal of Immunotoxicology

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Artemisia species are important medicinal plants throughout the world. The present in vitro study, using a sesquiterpene lactone-bearing fraction prepared from Artemisia khorassanica (SLAK), sought to investigate immunomodulatory/anti-inflammatory properties of this plant and elucidate potential underlying mechanisms for the actions. Effects of the SLAK on mitogeninduced murine splenocyte proliferation and interleukin (IL)-4 and interferon (IFN)-secretion were evaluated. To assess anti-inflammatory activities, levels of inducible of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ), as well as expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in peritoneal macrophages was examined. The results showed that SLAK noticeably was capable of suppressing PHA/LPS-stimulated splenocyte proliferation and of up-regulating production of the T-helper (T H )-2 cell cytokine IL-4 while down-regulating formation of T H 1 IFN. In addition, while SLAK caused negligible proliferation inhibition, peritoneal macrophages displayed considerable decrease in NO and PGE 2 production along with iNOS and COX-2 expression. The current experiment shows Artemisia khorasanica -a traditionally used herb -may have immunomodulatory and anti-inflammatory effects. It is anticipated that the ingredients may be employed as therapeutic candidates in the regulation of some immune responses implicated in various conditions and ailments.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

Rabe¹,

Iranshahi

Rastin³

et al. 2014

Journal of Immunotoxicology

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“…It's known that DHA induces cancer cell apoptosis via caspase-3-dependent mitochondrial pathway (Lu et al, 2009) To determine whether caspase-3 was involved in DHA and Dic co-induced apoptosis, cells were preincubated with 10 μM z-DQMD-fmk for 1 h before drugs treatment. As shown in Figure 6, pretreatment with z-DQMD-fmk significantly attenuated not only DHAinduced apoptosis but also Dic and DHA co-induced apoptosis, implying that caspase-3 play an important role in Dic and DHA co-induced A549 cell apoptosis.…”

Section: Dha Enhances Dic-induced Cell Apoptosis Via Caspase-3 Dependmentioning

confidence: 99%

“…Many studies showed that DHA can inhibit proliferation and induce apoptosis via caspase-3-dependent mitochondrial pathway (Lu et al, 2009). However, it is not unclear whether Dic and DHA have synergistic effect on inducing cancer cell apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Dihydroartemisinine Enhances Dictamnine-induced Apoptosis via a Caspase Dependent Pathway in Human Lung Adenocarcinoma A549 Cells

An¹,

Liu²,

Zhang³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Dictamnine (Dic) has the ability to exert cytotoxicity in human cervix, colon, and oral carcinoma cells and dihydroartemisinin (DHA) also has potent anticancer activity on various tumour cell lines. This report explores the molecular mechanisms by which Dic treatment and combination treatment with DHA and Dic cause apoptosis in human lung adenocarcinoma A549 cells. Dic treatment induced concentration-and time-dependent cell death. FCM analysis showed that Dic induced S phase cell cycle arrest at low concentration and cell apoptosis at high concentration in which loss of mitochondrial membrane potential (ΔΨm) was not involved. In addition, inhibition of caspase-3 using the specific inhibitor, z-DQMD-fmk, did not attenuate Dic-induced apoptosis, implying that Dic-induced caspase-3-independent apoptosis. Combination treatment with DHA and Dic dramatically increased the apoptotic cell death compared to Dic alone. Interestingly, pretreatment with z-DQMD-fmk significantly attenuated DHA and Dic co-induced apoptosis, implying that caspase-3 plays an important role in Dic and DHA co-induced cell apoptosis. Collectively, we found that Dic induced S phase cell cycle arrest at low concentration and cell apoptosis at high concentration in which mitochondria and caspase were not involved and DHA enhanced Dic induced A549 cell apoptosis via a caspase-dependent pathway.

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“…The Artemisia genus has been used in folk medicine for many years; numerous reports have indicated a vast range of biological effects associated with different Artemisia species (Abad Martinez et al, 2012;Choi et al, 2013). Among the various chemical agents found in these plants, sesquiterpene lactones have been proven to impart anticancer effects (Tan et al, 1998;Firestone et al, 2009;Firestone 2009;Lu 2009;Zhai 2010;Feng 2013).…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer Properties of a Sesquiterpene Lactone-bearing Fraction from Artemisia khorassanica

Rabe

Emami²,

Iranshahi³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells

Cited by 116 publications

References 36 publications

In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

In vitroimmunomodulatory properties of a sesquiterpene lactone-bearing fraction fromArtemisia khorassanica

Dihydroartemisinine Enhances Dictamnine-induced Apoptosis via a Caspase Dependent Pathway in Human Lung Adenocarcinoma A549 Cells

Anti-cancer Properties of a Sesquiterpene Lactone-bearing Fraction from Artemisia khorassanica

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