Dihaploid plants of roses (Rosa x hybrida, cv 'Sonia') obtained by parthenogenesis induced using irradiated pollen and in vitro culture of immature seeds

Meynet, J.; Barrade, R.; Duclos, Aroune; Siadous, R.

doi:10.1051/agro:19940303

Cited by 39 publications

(20 citation statements)

References 3 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The gynogenetic haploid was obtained by fertilizing a pentaploid specimen of R. canina with pollen that had been rendered genomically unstable by irradiation with g-rays (600 Gy), and rescuing the embryo in vitro (Meynet et al, 1994). Herbarium specimens of R. canina and the gynogenetic haploid are at Queen Mary, University of London.…”

Section: Methodsmentioning

confidence: 99%

Evolutionary implications of permanent odd polyploidy in the stable sexual, pentaploid of Rosa canina L

et al. 2005

View full text Add to dashboard Cite

In Rosa canina (2n ¼ 5x ¼ 35), the pollen and ovular parents contribute, respectively, seven and 28 chromosomes to the zygote. At meiosis I, 14 chromosomes form seven bivalents and 21 chromosomes remain as univalents. Fluorescent in situ hybridization to mitotic and pollen mother cells (PMC) of R. canina showed that 10 chromosomes (two per genome) carry ribosomal DNA (rDNA) loci. Five chromosomes carry terminal 18S-5.8S-26S rDNA loci; three of these also carry paracentric 5S rDNA loci and were designated as marker chromosomes 1. Five chromosomes carry only 5S rDNA loci and three of these were designated as marker chromosomes 2. The remaining four of the 10 chromosomes with rDNA loci were individually identifiable by the type and relative sizes of their rDNA loci and were numbered separately. At PMC meiosis, two marker chromosomes 1 and two marker chromosomes 2 formed bivalents, whereas the others were unpaired. In a gynogenetic haploid of R. canina (n ¼ 4x ¼ 28), obtained after pollination with g-irradiated pollen, chromosomes at meiosis I in PMC remained predominantly unpaired. The data indicate only one pair of truly homologous genomes in R. canina. The 21 unpaired chromosomes probably remain as univalents through multiple generations and do not recombine. The long-term evolutionary consequence for the univalents is likely to be genetic degradation through accumulated mutational change as in the mammalian Y chromosome and chromosomes of asexual species. But there is no indication that univalents carry degenerate 5S rDNA families. This may point to a recent evolution of the R. canina meiotic system. Heredity (2005) 94, 501-506.

show abstract

Section: Methodsmentioning

confidence: 99%

Evolutionary implications of permanent odd polyploidy in the stable sexual, pentaploid of Rosa canina L

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The diversity might be higher in the HW population, as it was derived from an interspeciWc cross of the dihaploid H190 (obtained from haploidisation of the tetraploid Rosa hybrida cv. Zambra, Meynet et al 1994) and a hybrid of a diploid R. wichurana genotype. In contrast, the 94/1 and 97/7 both originate from intraspeciWc crosses (Yan et al 2005;Linde et al 2006).…”

Section: Chr5mentioning

confidence: 99%

Towards a unified genetic map for diploid roses

Spiller

Linde

Oyant³

et al. 2010

Theor Appl Genet

150

View full text Add to dashboard Cite

We have constructed the Wrst integrated consensus map (ICM) for rose, based on the information of four diploid populations and more than 1,000 initial markers. The single population maps are linked via 59 bridge markers, on average 8.4 per linkage group (LG). The integrated map comprises 597 markers, 206 of which are sequence-based, distributed over a length of 530 cM on seven LGs. By using a larger eVective population size and therefore higher marker density, the marker order in the ICM is more reliable than in the single population maps. This is supported by a more even marker distribution and a decrease in gap sizes in the consensus map as compared to the single population maps. This uniWed map establishes a standard nomenclature for rose LGs, and presents the location of important ornamental traits, such as self-incompatibility, black spot resistance (Rdr1), scent production and recurrent blooming. In total, the consensus map includes locations for 10 phenotypic single loci, QTLs for 7 diVerent traits and 51 ESTs or gene-based molecular markers. This consensus map combines for the Wrst time the information for traits with high relevance for rose variety development. It will serve as a tool for selective breeding and marker assisted selection. It will beneWt future eVorts of the rose community to sequence the whole rose genome and will be useful for synteny studies in the Rosaceae family and especially in the section Rosoideae.

show abstract

“…H190 resulted from the haploidisation of the 4x R. hybrida cv Zambra. It was obtained by in situ parthenogenesis induced by using irradiated pollen (γ-rays at 600 Gy) and embryo rescue, as described by Meynet et al (1994). H190 is a recurrent blooming, double-flowered and thornless rose, whereas R. wichuraiana is single seasonal blooming, single flowered and thorny.…”

Section: Plant Materialsmentioning

confidence: 99%

Mapping of qualitative and quantitative phenotypic traits in Rosa using AFLP markers

Crespel¹,

Chirollet²,

Durel

et al. 2002

Theor Appl Genet

Self Cite

116

View full text Add to dashboard Cite

A segregating population of 91 hybrids issued from a cross between a dihaploid rose, derived from the haploidisation of a modern cultivar, and a diploid species was used to construct linkage maps of the parental genomes. As in other recent genetic studies in Rosa, AFLPs were used as molecular markers. Two segregating qualitative traits, recurrent blooming and double corolla, already known to be inherited as single recessive and dominant genes, respectively, were recorded in the mapping population. A quantitative trait, thorn density of the shoots, was also evaluated in this population. Sixty eight and 108 AFLP markers located on 8 and 6 linkage groups could be analysed in the female and male parent, respectively. The two recorded qualitative phenotypic markers were mapped as well as the quantitative one, after having performed QTL analyses on the parental maps in the latter case. It appears that thorn quantity is controlled by a major and a minor QTL which are located on the same linkage group at 36.5 and 3.2 cM from the single seasonal-blooming gene, respectively.

show abstract

Dihaploid plants of roses (Rosa x hybrida, cv 'Sonia') obtained by parthenogenesis induced using irradiated pollen and in vitro culture of immature seeds

Abstract: Summary -'Sonia' var rose ovules produced plants through in

Cited by 39 publications

References 3 publications

Evolutionary implications of permanent odd polyploidy in the stable sexual, pentaploid of Rosa canina L

Evolutionary implications of permanent odd polyploidy in the stable sexual, pentaploid of Rosa canina L

Towards a unified genetic map for diploid roses

Mapping of qualitative and quantitative phenotypic traits in Rosa using AFLP markers

Contact Info

Product

Resources

About