Digoxin immunoreactivity in cord and maternal serum and placental extracts. partial characterization of immunoreactive substances by high-performance liquid chromatography and inhibition of Na+, K+-ATPase

Diamandis, Eleftherios P.; Papanastasiou-Diamandi, A.; Soldin, Steven J.

doi:10.1016/s0009-9120(85)80023-0

Cited by 67 publications

(25 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Very few studies have been published on DLISs isolated from human tissues. Beyers et al [12] and Diamandis et al [9] extracted DLIS from human placenta. Spiehler et al…”

Section: Resultsmentioning

confidence: 99%

“…We did not attempt to characterize the DLIS that was not retained by the Sep Pak®. This method was convenient and provided us with a clean, concen trated extract for HPLC studies [9],…”

Section: Cord Blood Extractionmentioning

confidence: 99%

“…This substance(s) is of fetal origin and serum levels correlate neg atively with gestational age [5,11], Some workers [9,12] have suggested that DLIS might be among the pathologic factors in volved in hyponatremia encountered in pre term infants [13][14][15]. Other studies have suggested that DLIS may be involved in the etiology of pre-eclampsia [12,16,17], In this regard, it has been suggested that the DLIS found in toxemia may inhibit Na+/KfATPase activity and be the 'putative natri uretic factor' which is under consideration in the etiology of essential hypertension [18].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Study into the Nature and Organ Source of Digoxin-Like Immunoreactive Substance(s) in the Perinatal Period

et al. 1989

View full text Add to dashboard Cite

Digoxin-like immunoreactive substance(s) (DLIS) was isolated from sera and autopsy-derived tissue obtained from premature and full-term neonates. The highest tissue level of DLIS was in the small bowel followed by the adrenal, gallbladder and liver. Of the fluids examined, meconium had the highest level of DLIS. Preparative high performance liquid chromatography fractionation of cord blood generated at least six different fractions which not only contained DLIS material but also inhibited canine kidney Na⁺/K⁺-ATPase activity. Recovery/inhibition studies indicated that 72% of the canine kidney Na⁺/K⁺-ATPase inhibition within one fraction could be accounted for on the basis of progesterone content of the fraction.

show abstract

“…Very few studies have been published on DLISs isolated from human tissues. Beyers et al [12] and Diamandis et al [9] extracted DLIS from human placenta. Spiehler et al…”

Section: Resultsmentioning

confidence: 99%

“…We did not attempt to characterize the DLIS that was not retained by the Sep Pak®. This method was convenient and provided us with a clean, concen trated extract for HPLC studies [9],…”

Section: Cord Blood Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Study into the Nature and Organ Source of Digoxin-Like Immunoreactive Substance(s) in the Perinatal Period

et al. 1989

View full text Add to dashboard Cite

show abstract

“…This is remarkable because steroid hormones and digitalis glycosides are similar in structure and steroid hormones have been suggested to account for at least part of the immunological digitalis-like activity in human plasma [23]. Together with the fact that our assay was described as not cross-reacting with steroids, the result argues against the possibility that DLIS represents a steroid hormone.…”

Section: Dlis -Impact Of Gestational Age and Effect Of Prenatal Steroidsmentioning

confidence: 69%

Digoxin-Like Immunoreactive Substance in Nonoliguric Hyperkalemia of the Premature Infant

et al. 2003

View full text Add to dashboard Cite

Nonoliguric hyperkalemia of premature infants probably results from a transient inhibition of membrane-bound Na⁺/K⁺-ATPase during the first 24 h after birth. We hypothesized that the endogenous digitalis-like activity of the serum of premature infants, which inhibits the Na⁺/K⁺-ATPase, triggered hyperkalemia. Serum concentrations of potassium ([K⁺]) and of the digoxin-like immunoreactive substance ([DLIS]) were measured during the first 24 h after birth in 60 infants including 30 infants <30 gestational weeks. Contrary to our hypothesis, there was a negative linear correlation between [DLIS] at birth and [K⁺] 24 h after birth (r² = 0.24, p < 0.002). 24 h after birth there was no correlation between [DLIS] and [K⁺]. Thus, a major role of DLIS in nonoliguric hyperkalemia could not be established.

show abstract

“…The rapid disappearance of EDLF from maternal blood postpartum, and its presence for several days after birth in fetal blood suggest a placental origin; 27 however, this was not found in two other studies. 28,29 Some form of EDLF has been extracted from placental tissue, 30,31 and in one study, an MBG-like factor was tentatively identified. 32 The demonstration that immediate curettage accelerates the recovery from preeclampsia 33 further suggests that the placenta contributes significantly to the abnormal pathophysiology of preeclampsia, possibly by secreting EDLF.…”

Section: Discussionmentioning

confidence: 99%

The hemodynamic effects of intravenous digoxin-binding fab immunoglobulin in severe preeclampsia: a double-blind, randomized, clinical trial

et al. 2009

View full text Add to dashboard Cite

Objective: An endogenous digitalis-like factor (EDLF) has been implicated in the pathophysiology of preeclampsia (PE). This hypothesis is supported by two cases of preeclampsia in which administration of digoxin immune Fab (DIF) reduced mean arterial pressure (MAP). Study Design: To study this observation further, we performed a double-blind, placebo-controlled, randomized clinical trial to examine the effects on MAP of intravenous DIF given after delivery in 26 subjects with severe preeclampsia. Treating obstetricians were blinded to subject assignment and were allowed to use standard antihypertensive drugs during the trial. Results: The primary outcome, a significant difference in blood pressure between the two groups over the 24-h period of observation after the intervention, was not supported. However, mean MAP was significantly lower in the DIF-treated subjects for the first 4 h after therapy as compared with controls (P ¼ 0.05). Six subjects (46.2%) in the placebo arm were given conventional antihypertensive medications by their obstetrician for blood pressure >160 mm Hg systolic or >110 mm Hg diastolic, compared with zero subjects in the treatment arm (P ¼ 0.01). A trend towards increased creatinine clearance was observed in DIF-treated subjects (137.6 ± 42.6 versus 104.1 ± 43.4, P ¼ 0.07). Conclusion: These results support the hypothesis that EDLF contributes to the elevated blood pressure in preeclampsia and suggests a possible role for DIF as a treatment for this condition.

show abstract

Digoxin immunoreactivity in cord and maternal serum and placental extracts. partial characterization of immunoreactive substances by high-performance liquid chromatography and inhibition of Na+, K+-ATPase

Cited by 67 publications

References 13 publications

A Study into the Nature and Organ Source of Digoxin-Like Immunoreactive Substance(s) in the Perinatal Period

A Study into the Nature and Organ Source of Digoxin-Like Immunoreactive Substance(s) in the Perinatal Period

Digoxin-Like Immunoreactive Substance in Nonoliguric Hyperkalemia of the Premature Infant

The hemodynamic effects of intravenous digoxin-binding fab immunoglobulin in severe preeclampsia: a double-blind, randomized, clinical trial

Contact Info

Product

Resources

About