Diglons are heterodimeric proteins composed of IgLON subunits, and Diglon-CO inhibits neurite outgrowth from cerebellar granule cells

Reed, James E.; McNamee, Christine J.; Rackstraw, Stephen A.; Jenkins, Jennifer; Moss, Darren M.

doi:10.1242/jcs.01261

Cited by 50 publications

(52 citation statements)

References 39 publications

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“…It is novel in various CAMs that homophilic and heterophilic cis-interactions are important for trans-interactions to control neuronal outgrowth and synaptogenesis. Four IgLON CAMs are able to form six heterophilic dimer called Diglons, 16 therefore, it is possible that homophilic and heterophilic cis-interactions of IgLON proteins at postsynaptic sites are crucial to modulate the synaptic formation through presynaptic partners of IgLON proteins. Data represent means AE s.e.m obtained from 13 control (total analyzed length; 2050 mm), 9 Kilon-expressing (1700 mm), 19 LAMP-expressing (3200 mm), 9 Ntm-expressing (1500 mm), 12 OBCAM-expressing (1400 mm), 12 Kilon-LAMP-expressing (2150 mm), 11 Kilon-Ntm-expressing (1950 mm), 11 Kilon-OBCAM-expressing (1850 mm), 13 LAMP-Ntmexpressing (2600 mm), 10 LAMP-OBCAM-expressing (1700 mm), and 12 Kilon-expressing neurons (2600 mm).…”

Section: Resultsmentioning

confidence: 99%

IgLON cell adhesion molecules regulate synaptogenesis in hippocampal neurons

Hashimoto

Maékawa

Miyata

2009

Cell Biochemistry & Function

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IgLON cell adhesion molecules (CAMs) belonging to the immunoglobulin superfamily comprise of LAMP, neurotrimin (Ntm), OBCAM, and Kilon. In the present study, we performed the single and double transfection of IgLON gene constructs into hippocampal neurons in vitro and evaluated synaptic number. The quantitative analysis showed that the single over-expression of LAMP or OBCAM increased synaptic number, while the over-expression of Kilon reduced synaptic number and Ntm had no effects. The double over-expression of Kilon-Ntm, Kilon-OBCAM, LAMP-Ntm, and Ntm-OBCAM decreased synaptic number and that of Kilon-LAMP and LAMP-OBCAM had no effect. These results suggest that IgLON CAMs participate in regulating synapse formation in hippocampal neurons.

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Section: Resultsmentioning

confidence: 99%

IgLON cell adhesion molecules regulate synaptogenesis in hippocampal neurons

Hashimoto

Maékawa

Miyata

2009

Cell Biochemistry & Function

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“…5B) where they showed overlapping expression patterns. The three interacting GPI-linked proteins belonging to the IgLON family (Reed et al 2004) were expressed in complementary territories in the zebrafish head, suggesting roles in patterning the embryonic brain (Fig. 5C).…”

Section: Extracellular Receptor-ligand Pairs Are Enriched For Paralogsmentioning

confidence: 98%

Large-scale screening for novel low-affinity extracellular protein interactions

Söllner²,

et al. 2008

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Extracellular protein-protein interactions are essential for both intercellular communication and cohesion within multicellular organisms. Approximately a fifth of human genes encode membrane-tethered or secreted proteins, but they are largely absent from recent large-scale protein interaction datasets, making current interaction networks biased and incomplete. This discrepancy is due to the unsuitability of popular high-throughput methods to detect extracellular interactions because of the biochemical intractability of membrane proteins and their interactions. For example, cell surface proteins contain insoluble hydrophobic transmembrane regions, and their extracellular interactions are often highly transient, having half-lives of less than a second. To detect transient extracellular interactions on a large scale, we developed AVEXIS (avidity-based extracellular interaction screen), a high-throughput assay that overcomes these technical issues and can detect very transient interactions (halflives Յ 0.1 sec) with a low false-positive rate. We used it to systematically screen for receptor-ligand pairs within the zebrafish immunoglobulin superfamily and identified novel ligands for both well-known and orphan receptors. Genes encoding receptor-ligand pairs were often clustered phylogenetically and expressed in the same or adjacent tissues, immediately implying their involvement in similar biological processes. Using AVEXIS, we have determined the first systematic low-affinity extracellular protein interaction network, supported by independent biological data. This technique will now allow large-scale extracellular protein interaction mapping in a broad range of experimental contexts.

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“…The effects of IgLON family members vary depending on the cell type and stage (20,33). We coated IgLONs as substrates and tested their effects on neurite outgrowth from 8-DIV cortical neurons (20).…”

Section: Immobilized Full-length Iglon Family Members Promote Neuritementioning

confidence: 99%

IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth

Sanz

Ferraro

Fournier

2015

Journal of Biological Chemistry

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Background: Ectodomain shedding by the metalloproteinase family affects axon guidance and neurite outgrowth. Results: IgLON family members are metalloproteinase substrates that promote neurite outgrowth in a metalloproteinase-dependent manner. Conclusion: IgON family members are shed from the surface of cortical neurons to promote neurite extension. Significance: Proteolytic cleavage of IgLON family members could have critical roles in specific targeting and synaptogenesis in cortical neurons.

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Diglons are heterodimeric proteins composed of IgLON subunits, and Diglon-CO inhibits neurite outgrowth from cerebellar granule cells

Cited by 50 publications

References 39 publications

IgLON cell adhesion molecules regulate synaptogenesis in hippocampal neurons

IgLON cell adhesion molecules regulate synaptogenesis in hippocampal neurons

Large-scale screening for novel low-affinity extracellular protein interactions

IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth

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