Digital techniques for luminescence detection in liquid chromatography with an intensified linear photodiode array

Objective: The objective of the present study is to establish a simple, sensitive, and specific RP-HPLC technique to determine atorvastatin calcium (ATV) and atenolol (ATL) in rabbit plasma. Methods: Both the drugs like atorvastatin calcium and atenolol have been extracted by simple liquid-liquid extraction with acetonitrile. Results: The chromatographic separation has performed on a reversed-phase C18 column with a mobile phase of phosphate buffer (pH 4.0): acetonitrile: methanol (40:40:20, v/v). The techniques have been validated over the concentration range of 22.08-4778.37 ng ml-1 for ATV and 18.35-3969.20 ng ml-1 for ATL in rabbit plasma. The acceptability range was satisfied for all validation criteria. These methods have been profitably applied to the pharmacokinetic study of a bilayer tablet of ATV and ATL after an oral administration to a rabbit. A significant recovery of the drug from plasma resulted from acetonitrile as extracting solvent compared to other organic solvents. Conclusion: The results obtained proved that the RP-HPLC method developed was accurate and reproducible, and the drug was stable in rabbit plasma.

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Digital techniques for luminescence detection in liquid chromatography with an intensified linear photodiode array

Cited by 3 publications

References 14 publications

Nuclear and radiochemical analysis

Nuclear and radiochemical analysis

Validated Reversed‐Phase HPLC Method for the Determination of Atenolol in the Presence of Its Major Degradation Product

Simultaneous Determination of Atorvastatin and Atenolol in Rabbit Plasma by Rp-HPLC Method and Its Application in Pharmacokinetic Study

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