Digital quantification of fibrosis in liver biopsy sections: Description of a new method by Photoshop software

Dahab, Gamal M.; Kheriza, Mohamed M; El-Beltagi, Hussien M; Fouda, Abdel-Motaal; El-Din, Osama A Sharaf

doi:10.1111/j.1440-1746.2004.03183.x

Cited by 87 publications

(79 citation statements)

References 31 publications

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“…53 The cortical area of the entire biopsy was analyzed in a stepwise fashion as a series of consecutive fields, with a 20ϫ objective, avoiding the capsule, the subcapsular areas, and the arterial adventitia. The percentage of green-stained area ([fibrous tissue]/total area) was measured.…”

Section: Morphometric Analysismentioning

confidence: 99%

The Anti-Fibrotic Effect of Mycophenolic Acid–Induced Neutral Endopeptidase

Dell'Oglio

Zaza

Rossini

et al. 2010

Journal of the American Society of Nephrology

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Mycophenolic acid (MPA) appears to have anti-fibrotic effects, but the molecular mechanisms underlying this are unknown. We prospectively studied 35 stable kidney transplant recipients maintained on cyclosporine and azathioprine. We converted 20 patients from azathioprine to enteric-coated mycophenolate sodium (EC-MPS) and continued the remaining 15 patients on azathioprine. Exploratory mRNA expression profiling, performed on five randomly selected EC-MPS patients, revealed significant upregulation of neutral endopeptidase (NEP), which is an enzyme that degrades angiotensin II. We confirmed these microarray data by measuring levels of NEP expression in all subjects; in addition, we found that NEP gene expression correlated inversely with proteinuria. In an additional 33 patients, glomerular and tubular NEP protein levels from renal graft biopsies were significantly higher among the 13 patients receiving cyclosporine ϩ EC-MPS than among the 12 patients receiving cyclosporine ϩ azathioprine or 8 patients receiving cyclosporine alone. Glomerular NEP expression inversely correlated with glomerulosclerosis and proteinuria, and tubular NEP expression inversely correlated with interstitial fibrosis. Incubation of human proximal tubular cells with MPA increased NEP gene expression in a doseand time-dependent manner. Moreover, MPA reduced angiotensin II-induced expression of the profibrotic factor plasminogen activator inhibitor-1, and a specific NEP inhibitor completely reversed this effect. Taken together, our data suggest that MPA directly induces expression of neutral endopeptidase, which may reduce proteinuria and slow the progression of renal damage in kidney transplant recipients. 21: 215721: -216821: , 201021: . doi: 10.1681 Over the last decade, the continuous progress in the development of immunosuppressive agents has enhanced both the efficacy and safety of antirejection regimens after renal transplantation, leading to a dramatic reduction in the incidence of acute rejection. 1 This significant improvement in short-term graft outcome was not followed by a similar advance in preventing graft loss caused by chronic graft injury. 2,3 The addition of mycophenolic acid (MPA) into contemporary immunosuppressive regimens represented an important step forward in the development of new therapeutic protocols in kidney transplantation. J Am Soc NephrolMPA exerts its immunosuppressive effect by inhibiting the inosine 5Ј monophosphate dehydro-

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Section: Morphometric Analysismentioning

confidence: 99%

The Anti-Fibrotic Effect of Mycophenolic Acid–Induced Neutral Endopeptidase

Dell'Oglio

Zaza

Rossini

et al. 2010

Journal of the American Society of Nephrology

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“…Computer-aided quantification of ICC staining was performed based on the principle originally described by Dahab et al [20] with slight modification [21] . Briefly, digital images were acquired at 100× using a Leica digital camera and were analyzed with VideoTesT-Morphology software (Russia).…”

Section: Digital Image Analysismentioning

confidence: 99%

Reduction of mitomycin C resistance in human bladder cancer T24 cells by knocking-down ras oncogene

Eldin¹,

Fouda²,

Youssef³

et al. 2018

CDR

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Aim: Mitomycin C (MMC) is a commonly used as intravesical treatment for superficial bladder cancer. However, its role in combination with ras inhibition has not been investigated. The aim of this study was to explore the role of ras in MMCinduced apoptosis in T24 bladder cancer cells and to determine the efficacy of combination therapy in vitro . Methods:We measured the effects of various doses of MMC on apoptosis induction as well as on ras, ERK and Ki-67 protein expression by T24 cell line using immunocytochemistry, flow cytometry and Western blotting. We also tested the effect of siRNA on ras employed singly or in combination with MMC. Results: T24 cells expressed high level of ras protein. MMC treatment increased the level of ras and ERK protein expressionafter 24 h, and decreased these levels after 72 h. Ras siRNA (100 nmol/L) caused massive apoptosis associated with a marked decrease in ras expression in T24 cells. When combined with low doses of MMC, ras siRNA (50 nmol/L) sensitized T24 cells to apoptosis and decreased their expression of ras. The effect of combined therapy was higher than that of either compound used alone. Expression levels of ERK, a downstream target of ras, declined following combination therapy. Conclusion:Ras siRNA in combination with low dose MMC is a possible treatment strategy for patients with ras-positive bladder tumors.

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“…21 The fibrosis ratio, the total area of fibrosis within the aneurysmal cavity and neck divided by the total area of the aneurysmal cavity and neck, was calculated for each aneurysm. We analyzed the data using 2-way analysis of variance (least squares fit; JMP, SAS Institute).…”

Section: Collagen Depositionmentioning

confidence: 99%

Endovascular Treatment of Experimental Aneurysms with Use of Fibroblast Transfected with Replication-Deficient Adenovirus Containing Bone Morphogenetic Protein-13 Gene

Dai

Ding

Danielson

et al. 2008

AJNR Am J Neuroradiol

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BACKGROUND AND PURPOSE:Modified coils have failed to improve long-term recanalization of aneurysms. This study examined whether ex vivo transduction of replication-deficient adenovirus containing the bone morphogenetic protein-13 gene (Ad-BMP-13) in fibroblast allografts would improve angiographic results via increased collagen synthesis, compared with fibroblast-coated platinum coils (FBC) and bare platinum coils (PA).

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Digital quantification of fibrosis in liver biopsy sections: Description of a new method by Photoshop software

Cited by 87 publications

References 31 publications

The Anti-Fibrotic Effect of Mycophenolic Acid–Induced Neutral Endopeptidase

The Anti-Fibrotic Effect of Mycophenolic Acid–Induced Neutral Endopeptidase

Reduction of mitomycin C resistance in human bladder cancer T24 cells by knocking-down ras oncogene

Endovascular Treatment of Experimental Aneurysms with Use of Fibroblast Transfected with Replication-Deficient Adenovirus Containing Bone Morphogenetic Protein-13 Gene

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