Digital PCR on a SlipChip

Shen, Feng; Du, Wenbin; Kreutz, Jason E.; Fok, Alice; Ismagilov, Rustem F.

doi:10.1039/c004521g

Cited by 257 publications

(264 citation statements)

References 64 publications

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“…This is a good test because loading SlipChip devices requires control of the inlet pressure within a defined range, 25 and SlipChips are intended to be used in limited resource settings (LRS) by untrained users. [26][27][28][29] First, we tested the pumping lid on a SlipChip designed for a digital nucleic acid detection assay 26 (Fig. 6A), pumping a total of 5 μL of solution with 0.03 atm pressure (eqn (1)).…”

Section: Use Of Pumping Lids To Load Slipchip Devices By Positive Andmentioning

confidence: 99%

The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications

et al. 2014

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Equipment-free pumping is a challenging problem and an active area of research in microfluidics, with applications for both laboratory and limited-resource settings. This paper describes the pumping lid method, a strategy to achieve equipment-free pumping by controlled generation of pressure. Pressure was generated using portable, lightweight, and disposable parts that can be integrated with existing microfluidic devices to simplify workflow and eliminate the need for pumping equipment. The development of this method was enabled by multi-material 3D printing, which allows fast prototyping, including composite parts that combine materials with different mechanical properties (e.g. both rigid and elastic materials in the same part). The first type of pumping lid we describe was used to produce predictable positive or negative pressures via controlled compression or expansion of gases. A model was developed to describe the pressures and flow rates generated with this approach and it was validated experimentally. Pressures were pre-programmed by the geometry of the parts and could be tuned further even while the experiment was in progress. Using multiple lids or a composite lid with different inlets enabled several solutions to be pumped independently in a single device. The second type of pumping lid, which relied on vapor-liquid equilibrium to generate pressure, was designed, modeled, and experimentally characterized. The pumping lid method was validated by controlling flow in different types of microfluidic applications, including the production of droplets, control of laminar flow profiles, and loading of SlipChip devices. We believe that applying the pumping lid methodology to existing microfluidic devices will enhance their use as portable diagnostic tools in limited resource settings as well as accelerate adoption of microfluidics in laboratories.

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Section: Use Of Pumping Lids To Load Slipchip Devices By Positive Andmentioning

confidence: 99%

The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications

et al. 2014

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“…No cross-contamination between wells is observed. The SlipChip has already been successfully used for protein crystallization screening [62] and digital PCR [63]. …”

Section: "Valve-less" Approachesmentioning

confidence: 99%

Microvalves and Micropumps for BioMEMS

et al. 2011

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Abstract:This review presents an extensive overview of a large number of microvalve and micropump designs with great variability in performance and operation. The performance of a given design varies greatly depending on the particular assembly procedure and there is no standardized performance test against which all microvalves and micropumps can be compared. We present the designs with a historical perspective and provide insight into their advantages and limitations for biomedical uses.

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“…(Du et al, 2009;Shen et al, 2010). Here, two plates are set in contact, with one containing micro wells with reagents confined within an immiscible carrier fluid and an array of disconnected ducts.…”

Section: Transport / Reactionmentioning

confidence: 99%

Towards autonomous lab-on-a-chip devices for cell phone biosensing

Comina

Suska

Filippini

2016

Biosensors and Bioelectronics

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Modern cell phones are a ubiquitous resource with a residual capacity to accommodate chemical sensing and biosensing capabilities. From the different approaches explored to capitalize on such resource, the use of autonomous disposable lab-on-a-chip (LOC) devices conceived as only accessories to complement cell phones underscores the possibility to entirely retain cell phones ubiquity for distributed biosensing. The technology and principles exploited for autonomous LOC devices are here selected and reviewed focusing on their potential to serve cell phone readout configurations. Together with this requirement, the central aspects of cell phones resources that determine their potential for analytical detection are examined. The conversion of these LOC concepts into universal architectures that are readable on unaccessorized phones is discussed within this context. (C) 2015 Elsevier B.V. All rights reserved.

Funding Agencies|Swedish Research Council (VR) [C0453801]; Carl Tryggers Foundation [CTS14140]

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Digital PCR on a SlipChip

Cited by 257 publications

References 64 publications

The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications

The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications

Microvalves and Micropumps for BioMEMS

Towards autonomous lab-on-a-chip devices for cell phone biosensing

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