Digital pathology with Fourier ptychography

Horstmeyer, Roarke; Ou, Xiaoze; Zheng, Guoan; Willems, P. A.; Yang, Changhuei

doi:10.1016/j.compmedimag.2014.11.005

Cited by 91 publications

(43 citation statements)

References 17 publications

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“…Instead of starting with HR and stitching together a larger FOV, FP uses low numerical aperture (NA) objective to take advantage of its innate large FOV and stitches together low resolution (LR) images in Fourier space to recover HR by replacing the optical condensers of microscopes with the LED arrays. Due to its flexible setup, promising performance without mechanical scanning and interferometric measurements, FP has wide applications in the digital pathology [12], whole slide imaging systems [13] and combined with fluorescence imaging [14,15].…”

Section: Introductionmentioning

confidence: 99%

Subwavelength resolution Fourier ptychography with hemispherical digital condensers

Pan¹,

Zhang²,

Wen³

et al. 2018

Opt. Express

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Fourier ptychography (FP) is a promising computational imaging technique that overcomes the physical space-bandwidth product (SBP) limit of a conventional microscope by applying angular-varied illuminations. However, to date, the effective imaging numerical aperture (NA) achievable with a commercial LED board is still limited to the range of 0.3-0.7 with a 4 × /0.1NA objective due to the geometric constraint with the declined illumination intensities and attenuated signal-to-noise ratio (SNR). Thus the highest achievable half-pitch resolution is usually constrained between 500-1000 nm, which cannot meet the requirements of high-resolution biomedical imaging applications. Although it is possible to improve the resolution by using a high-NA objective lens, the FP approach is less appealing as the decrease of field-of-view (FOV) will far exceed the improvement of spatial resolution in this case. In this paper, we initially present a subwavelength resolution Fourier ptychography (SRFP) platform with a hemispherical digital condenser to provide high-angle programmable plane-wave illuminations of 0.95NA, attaining a 4 × /0.1NA objective with the final effective imaging performance of 1.05NA at a half-pitch resolution of 244 nm with the incident wavelength of 465 nm across a wide FOV of 14.60 mm, corresponding to a SBP of 245 megapixels. Our work provides an essential step of FP towards high-throughput imaging applications.

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Section: Introductionmentioning

confidence: 99%

Subwavelength resolution Fourier ptychography with hemispherical digital condensers

Pan¹,

Zhang²,

Wen³

et al. 2018

Opt. Express

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show abstract

“…Compared with the conventional microscopy, the FPM can achieve HR, wide FOV and quantitative phase imaging [9]. Therefore, it has great potential in a variety of applications, such as biomedical medicine [15,6,3], characterizing unknown optical aberrations of lenses [1,10].…”

Section: Introductionmentioning

confidence: 99%

Fast light source misalignment correction of Fourier ptychographic microscopy

Zhou

Wang

et al. 2018

Imaging and Applied Optics 2018 (3D, AO, AIO, COSI, DH, IS, LACSEA, LS&C, MATH, pcAOP)

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Fourier ptychographic microscopy (FPM) is a newly developed computational imaging technique that can provide gigapixel images with both high resolution (HR) and wide field of view (FOV). However, the position misalignment of the LED array induces a degradation of the reconstructed image, especially in the regions away from the optical axis. In this paper, we propose a robust and fast method to correct the LED misalignment of the FPM, termed as misalignment correction for the FPM (mcFPM). Although different regions in the FOV have different sensitivity to the LED misalignment, the experimental results show that the mcFPM is robust with respect to the elimination of each region. Compared with the state-of-the-art methods, the mcFPM is much faster.

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“…In order to realize high-contrast and quantitative label-free cellular observation, phase imaging focusing on sample phase retrieval is proposed (Mir et al, 2012). Classical approaches as Gerchberg-Saxton algorithm (Gerchberg & Saxton, 1971) and Ptychographic methods including ptychographic iterative engine (Rodenburg & Faulkner, 2004;Rodenburg et al, 2007;Yu et al, 2016a,b>) and Fourier ptychographic microscopy (Horstmeyer et al, 2012;Horstmeyer & Yang, 2014;Kuang et al, 2015;Tian et al, 2015;Yeh et al, 2015;Zheng et al, 2013;Zhang et al, 2015a,b) acquire quantitative phase distributions from diffraction via iterative numerical wavefront propagations. Their lens-free systems are simple especially suiting for short-wavelength imaging.…”

Section: Introductionmentioning

confidence: 99%

Rapid in‐focus corrections on quantitative amplitude and phase imaging using transport of intensity equation method

Meng

Tian

Kong

et al. 2017

Journal of Microscopy

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Transport of intensity equation (TIE) method can acquire sample phase distributions with high speed and accuracy, offering another perspective for cellular observations and measurements. However, caused by incorrect focal plane determination, blurs and halos are induced, decreasing resolution and accuracy in both retrieved amplitude and phase information. In order to obtain high-accurate sample details, we propose TIE based in-focus correction technique for quantitative amplitude and phase imaging, which can locate focal plane and then retrieve both in-focus intensity and phase distributions combining with numerical wavefront extraction and propagation as well as physical image recorder translation. Certified by both numerical simulations and practical measurements, it is believed the proposed method not only captures high-accurate in-focus sample information, but also provides a potential way for fast autofocusing in microscopic system.

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Digital pathology with Fourier ptychography

Cited by 91 publications

References 17 publications

Subwavelength resolution Fourier ptychography with hemispherical digital condensers

Subwavelength resolution Fourier ptychography with hemispherical digital condensers

Fast light source misalignment correction of Fourier ptychographic microscopy

Rapid in‐focus corrections on quantitative amplitude and phase imaging using transport of intensity equation method

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