Digital Nanofluidic Chip for Simple and Highly Quantitative Detection of HPV Target
Li Liu,
Stephen J. Dollery,
Gregory J. Tobin
et al.
Abstract:Quantitative analysis of human papillomavirus (HPV)-infected cervical cancer is essential for early diagnosis and timely treatment of cervical cancer. Here, we introduce a novel energy transfer-labeled oligonucleotide probe to enhance the loop-mediated isothermal amplification (LAMP) assay for highly sensitive and specific detection of HPV 16. Conducted as a single-step assay within a digital nanofluidic chip featuring numerous reaction reservoirs, our method facilitates target amplification under isothermal c… Show more
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