Digital Microfluidic Sampler for a Portable Capillary Electropherograph

Gorbatšova, Jelena; Jaanus, Martin; Kaljurand, Mihkel

doi:10.1021/ac9015825

Cited by 29 publications

(15 citation statements)

References 32 publications

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“…The same strategy was used to couple DMF sample preparation to integrated microfluidic emitters for nanoelectrospray MS (79). In a related method, DMF was coupled with a portable capillary electrophoresis instrument (37). Sample droplets and run buffer droplets were actuated to the inlet of a separation capillary located on top of the electrode array.…”

Section: Integration With Other Techniquesmentioning

confidence: 99%

Digital Microfluidics

Choi

Fobel

et al. 2012

Annual Rev. Anal. Chem.

710

592

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Digital microfluidics (DMF) is an emerging liquid-handling technology that enables individual control over droplets on an open array of electrodes. These picoliter- to microliter-sized droplets, each serving as an isolated vessel for chemical processes, can be made to move, merge, split, and dispense from reservoirs. Because of its unique advantages, including simple instrumentation, flexible device geometry, and easy coupling with other technologies, DMF is being applied to a wide range of fields. In this review, we summarize the state of the art of DMF technology from the perspective of analytical chemistry in sections describing the theory of droplet actuation, device fabrication and integration, and applications.

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Section: Integration With Other Techniquesmentioning

confidence: 99%

Digital Microfluidics

Choi

Fobel

et al. 2012

Annual Rev. Anal. Chem.

710

592

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“…The fabrication of the DMF board used for transporting the droplets has been fully described by Gorbatsova et al . It consists of a printed circuit board with an H‐shaped set of 14 electrodes designed for various applications.…”

Section: Methodsmentioning

confidence: 99%

Electrowetting on dielectric actuation of droplets with capillary electrophoretic zones for MALDI mass spectrometric analysis

Gorbatšova

Borissova²,

Kaljurand

2012

Electrophoresis

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An automated fraction collection interface was developed for coupling CE with MALDI-MS. This fraction collection approach is based on the electrowetting on dielectric (EWOD) phenomenon performed on a digital microfluidic (DMF) board; it does not rely on a MALDI spotter. In this study, a four-peptide mixture was used as a sample test, and the separations were conducted in a portable CE instrument with a 150 μm o.d. × 50 μm i.d. capillary and a contactless conductivity detector. The CE instrument was interfaced with a robust DMF board. The CE fractions were directly deposited onto the DMF board at predetermined locations prior to MALDI analysis. The series of experiments determined the lowest concentration that produces a measurable MALDI signal. The concentrations were 0.25, 0.5, 0.05, and 0.05 nmol for bradykinin, angiotensin, ACTH (18-39), and insulin, respectively. The contactless conductivity detector limit of detection for the same analytes was 2.5 μmol.

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“…Capillary electrophoresis performs electroseparation of the ionic compounds, based on the size-tocharge ratio of the ions, which are transported electrokinetically in the carrier liquid. The electrocapillary forces drag the ions into the capillary channel while the fluidic sample approaches the inlet of the microchannel [ [102][103][104]. Capillary electrophoresis is capable of separating particulate substances like cells, amino acids, proteins, peptides, mitochondria, or bacteria [ 105 ].…”

Section: Advantages Of Lab-on-chip Devicesmentioning

confidence: 99%

Microfabrication of biomedical lab-on-chip devices. A review

Giannitsis

2011

Estonian J. Eng.

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Lab-on-chip systems are a class of miniaturized analytical devices that integrate fluidics, electronics and various sensorics. They are capable of analysing biochemical liquid samples, like solutions of metabolites, macromolecules, proteins, nucleic acids, or cells and viruses. Supplementary to their measuring capabilities, the lab-on-chip devices facilitate fluidic transportation, sorting, mixing, or separation of liquid samples. A type of lab-on-chip devices, named biochip, is devoted specifically to genomic, proteomic and pharmaceutical tests. The significance of such miniaturized devices lies in their potentiality of automating laboratory procedures, which highly reduces the time of biomedical tests and laboratory work. This review summarizes numerous fabrication methods and procedures for producing lab-on-chip devices and also envisages future evolution.

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Digital Microfluidic Sampler for a Portable Capillary Electropherograph

Cited by 29 publications

References 32 publications

Digital Microfluidics

Digital Microfluidics

Electrowetting on dielectric actuation of droplets with capillary electrophoretic zones for MALDI mass spectrometric analysis

Microfabrication of biomedical lab-on-chip devices. A review

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