Digital method for defocus corrections: experimental results

Raveh, Ido; Mendlovic, David; Zalevsky, Zeev; Lohmann, Adolf W.

doi:10.1117/1.602215

Cited by 18 publications

(4 citation statements)

References 6 publications

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“…In the method proposed here, approximated of the defocused ipsf's of the OS are obtained. However, other authors have used ipsf's numerically simulated [5,6] and to obtain them, the equation 3is used, where the defocus term ω 2,0 of the aberration polynomial is introduced. The defocus amount is calculated according with the equation (4), from the a priori knowledge of the distance from the object plane 0 z  at the moment in which the image is captured.…”

Section: Digital Methodsmentioning

confidence: 99%

“…When the Wiener filter is used, the mean square error in the comparison between the focused image and the restored one is minimum. For this reason we have decided to use this filter for obtaining the spectrum of the restored image [5,7]:…”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…In this work, a comparison of three different methods of restoration is presented. That which uses the qps and other two techniques using the digital (OTF's) [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of an optical-digital restoration technique with digital methods for microscopy defocused images

Ortiz-Sosa¹,

Berriel‐Valdos²,

Aguilar³

2017

RCS

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An optical-digital technique for restoring defocused images, and based on the quasi-point source concept, is introduced and compared with two conventional digital methods. The comparison is performed on the restoration of defocused microscopy images obtained with a conventional microscope but with three different numerical apertures. The effects of these techniques on the aberrated images are shown. We discuss and conclude about the advantages of our proposed technique.

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Section: Digital Methodsmentioning

confidence: 99%

Section: Theoretical Backgroundmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of an optical-digital restoration technique with digital methods for microscopy defocused images

Ortiz-Sosa¹,

Berriel‐Valdos²,

Aguilar³

2017

RCS

View full text Add to dashboard Cite

show abstract

“…Each phase correction will result in in-focus objects positioned at different axial distances. If digital postprocessing is considered, then the amount of defocusing can be estimated digitally from the image by observing the zeros generated in its Fourier plane [29,30].…”

Section: General Overview: Approaches To Extend the Depth Of Focusmentioning

confidence: 99%