Digital Light Processing-Based 3D Printing of Cell-Seeding Hydrogel Scaffolds with Regionally Varied Stiffness

Xue, Dongfeng; Zhang, Jiaxin; Wang, Yancheng; Mei, Deqing

doi:10.1021/acsbiomaterials.9b00696

Cited by 54 publications

(39 citation statements)

References 40 publications

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“…Excellent cell viability was found after seeding constructs and culturing for 21 days, however viability was found to decrease with increasing PEGDA concentration. Others have used PEGDA as a material to create cell encapsulated gradient features [ 183 ] and others explored its use in developing scaffolds with regionally varied stiffness, [ 186 ] bio‐inspired detoxification modules, [ 187 ] peripheral nerve guidance conduits. [ 188 ] Along with PEGDA other synthetic materials used for bioresins include PDLLA‐PEG‐PDLLA, poly(propylene fumarate) and PEG‐peptides.…”

Section: Bioresins and Recent Advancesmentioning

confidence: 99%

Next Evolution in Organ‐Scale Biofabrication: Bioresin Design for Rapid High‐Resolution Vat Polymerization

2022

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The field of bioprinting has made significant advancements in recent years and allowed for the precise deposition of biomaterials and cells. However, within this field lies a major challenge, which is developing high resolution constructs, with complex architectures. In an effort to overcome these challenges a biofabrication technique known as vat polymerization is being increasingly investigated due to its high fabrication accuracy and control of resolution (µm scale). Despite the progress made in developing hydrogel precursors for bioprinting techniques, such as extrusion‐based bioprinting, there is a major lack in developing hydrogel precursor bioresins for vat polymerization. This is due to the specific unique properties and characteristics required for vat polymerization, from lithography to the latest volumetric printing. This is of major concern as the shortage of bioresins available has a significant impact on progressing this technology and exploring its full potential, including speed, resolution, and scale. Therefore, this review discusses the key requirements that need to be addressed in successfully developing a bioresin. The influence of monomer architecture and bioresin composition on printability is described, along with key fundamental parameters that can be altered to increase printing accuracy. Finally, recent advancements in bioresins are discussed together with future directions.

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Section: Bioresins and Recent Advancesmentioning

confidence: 99%

Next Evolution in Organ‐Scale Biofabrication: Bioresin Design for Rapid High‐Resolution Vat Polymerization

2022

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“…It prints scaffolds by using light in a layer-by-layer fashion with the use of a rotatable digital micro-mirror device. 56 The light is projected onto a photosensitive polymer and uses the photopolymerization method to fabricate scaffolds. DLP is a high speed, high precision 3D printing technique and has given promising results in some BTE studies.…”

Section: Stereolithographymentioning

confidence: 99%

“…DLP 3D printing is a type of light‐assisted 3D printing method, like SLS and SLM. It prints scaffolds by using light in a layer‐by‐layer fashion with the use of a rotatable digital micro‐mirror device 56 . The light is projected onto a photosensitive polymer and uses the photopolymerization method to fabricate scaffolds.…”

Section: Scaffold Fabrication Using 3d Printingmentioning

confidence: 99%

Advances in 3D printing of composite scaffolds for the repairment of bone tissue associated defects

Anandhapadman¹,

Venkateswaran²,

Jayaraman³

et al. 2022

Biotechnology Progress

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The conventional methods of using autografts and allografts for repairing defects in bone, the osteochondral bone, and the cartilage tissue have many disadvantages, like donor site morbidity and shortage of donors. Moreover, only 30% of the implanted grafts are shown to be successful in treating the defects. Hence, exploring alternative techniques such as tissue engineering to treat bone tissue associated defects is promising as it eliminates the above‐mentioned limitations. To enhance the mechanical and biological properties of the tissue engineered product, it is essential to fabricate the scaffold used in tissue engineering by the combination of various biomaterials. Three‐dimensional (3D) printing, with its ability to print composite materials and with complex geometry seems to have a huge potential in scaffold fabrication technique for engineering bone associated tissues. This review summarizes the recent applications and future perspectives of 3D printing technologies in the fabrication of composite scaffolds used in bone, osteochondral, and cartilage tissue engineering. Key developments in the field of 3D printing technologies involves the incorporation of various biomaterials and cells in printing composite scaffolds mimicking physiologically relevant complex geometry and gradient porosity. Much recently, the emerging trend of printing smart scaffolds which can respond to external stimulus such as temperature, pH and magnetic field, known as 4D printing is gaining immense popularity and can be considered as the future of 3D printing applications in the field of tissue engineering.

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“…The synthetic materials based 3D hydrogel have also shown to mimic the native tissue stiffness while the optimum conditions for the 3D constructions are digitally controlled. The digital light processing (DLP) based printed poly(ethylene glycol) diacrylate (PEGDA) hydrogels exhibited nearly 60% of enhanced elastic modulus, suited for the support of 3 T3 cells adhesion and proliferation as shown in Figure 5A [54]. In one study, degradable, polar hydrophobic and ionic porous polyurethane scaffolds were synthesized using a lysine-based crosslinker.…”

Section: Different Synthetic Materials and Their Combinationmentioning

confidence: 99%

Current Scenario of Regenerative Medicine: Role of Cell, Scaffold and Growth Factor

Pramanik¹,

Rath²

2021

Biomechanics and Functional Tissue Engineering

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Impairment of the clinical tissue-implantation is due to the lack of a suitable organ donor and immunogenic rejection, which leads to the cause for the enormous loss of human life. The introduction of artificial regeneration of tissues by Langer and Vacanti in 1993, has revolutionized in the field of surgical organ transplantation, to alleviate the problem of tissue injury-related death. There is no doubt that the term “regenerative medicine” to open a new space of tissue reconstruction, but the complications that arise due to the proper machinery of the cell, supporting biomaterials and growth factors has yet to be resolved to expand its application in a versatile manner. The chapter would provide a significant overview of the artificial tissue regeneration while a triangular relationship between cells, matrixes, and growth factors should be established mentioning the necessity of biomedical tools as an alternative to organ transplantation.

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Digital Light Processing-Based 3D Printing of Cell-Seeding Hydrogel Scaffolds with Regionally Varied Stiffness

Cited by 54 publications

References 40 publications

Next Evolution in Organ‐Scale Biofabrication: Bioresin Design for Rapid High‐Resolution Vat Polymerization

Next Evolution in Organ‐Scale Biofabrication: Bioresin Design for Rapid High‐Resolution Vat Polymerization

Advances in 3D printing of composite scaffolds for the repairment of bone tissue associated defects

Current Scenario of Regenerative Medicine: Role of Cell, Scaffold and Growth Factor

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