Digital Light Processing 3D printing for biological applications of polydimethylsiloxane-based microfluidics

Poskus, Matthew D.; Wang, Tuo; Deng, Yuxuan; Borcherding, Sydney; Zervantonakis, Ioannis K.

doi:10.1101/2022.09.28.509779

Cited by 3 publications

(3 citation statements)

References 51 publications

(65 reference statements)

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“…3D printing methods (SLA, 2PP, FDM, DLP, and PolyJet) offer the rapid production [ 47 ] of microfluidic devices, forming complex channel structures, precise control over dimensions, and several materials, including tailored resins for diverse microfluidic applications [ 48 ]. Various microfluidic devices with different functionalities can be fabricated, including 3D microfluidics for cell culture [ 49 ], biosensing [ 50 ], drug delivery, inertial microfluidics [ 51 ], micromixers [ 16 ], and droplet-based microfluidics [ 52 ].…”

Section: D Printing Devices For Fabricating Microfluidic Systemsmentioning

confidence: 99%

Advancing 3D printed microfluidics with computational methods for sweat analysis

Ece,

Ölmez,

Hacıosmanoğlu

et al. 2024

Microchim Acta

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The intricate tapestry of biomarkers, including proteins, lipids, carbohydrates, vesicles, and nucleic acids within sweat, exhibits a profound correlation with the ones in the bloodstream. The facile extraction of samples from sweat glands has recently positioned sweat sampling at the forefront of non-invasive health monitoring and diagnostics. While extant platforms for sweat analysis exist, the imperative for portability, cost-effectiveness, ease of manufacture, and expeditious turnaround underscores the necessity for parameters that transcend conventional considerations. In this regard, 3D printed microfluidic devices emerge as promising systems, offering a harmonious fusion of attributes such as multifunctional integration, flexibility, biocompatibility, a controlled closed environment, and a minimal requisite analyte volume—features that leverage their prominence in the realm of sweat analysis. However, formidable challenges, including high throughput demands, chemical interactions intrinsic to the printing materials, size constraints, and durability concerns, beset the landscape of 3D printed microfluidic devices. Within this paradigm, we expound upon the foundational aspects of 3D printed microfluidic devices and proffer a distinctive perspective by delving into the computational study of printing materials utilizing density functional theory (DFT) and molecular dynamics (MD) methodologies. This multifaceted approach serves manifold purposes: (i) understanding the complexity of microfluidic systems, (ii) facilitating comprehensive analyses, (iii) saving both cost and time, (iv) improving design optimization, and (v) augmenting resolution. In a nutshell, the allure of 3D printing lies in its capacity for affordable and expeditious production, offering seamless integration of diverse components into microfluidic devices—a testament to their inherent utility in the domain of sweat analysis. The synergistic fusion of computational assessment methodologies with materials science not only optimizes analysis and production processes, but also expedites their widespread accessibility, ensuring continuous biomarker monitoring from sweat for end-users. Graphical Abstract

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Section: D Printing Devices For Fabricating Microfluidic Systemsmentioning

confidence: 99%

Advancing 3D printed microfluidics with computational methods for sweat analysis

Ece,

Ölmez,

Hacıosmanoğlu

et al. 2024

Microchim Acta

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“…Co., Ltd., Taiwan) features a very high printing resolution of 8K (7680 × 4320 pixels), which allows for extraordinary sharpness of detail and high precision. In addition, this printer also offers variable printing speed, which allows objects to be produced n a relatively short period of time [27].…”

Section: D Print Technologymentioning

confidence: 99%

Mechanical and Material Analysis of 3D-Printed Temporary Materials for Implant Reconstructions—A Pilot Study

Nowicki,

Osypko,

Kurzawa

et al. 2024

Biomedicines

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In this study, the authors analyzed modern resin materials typically used for temporary reconstructions on implants and manufactured via 3D printing. Three broadly used resins: NextDent Denture 3D, NextDent C&B MFH Bleach, and Graphy TC-80DP were selected for analysis and compared to currently used acrylic materials and ABS-like resin. In order to achieve this, mechanical tests were conducted, starting with the static tensile test PN-EN. After the mechanical tests, analysis of the chemical composition was performed and images of the SEM microstructure were taken. Moreover, numerical simulations were conducted to create numerical models of materials and compare the accuracy with the tensile test. The parameters obtained in the computational environment enabled more than 98% correspondence between numerical and experimental charts, which constitutes an important step towards the further development of numeric methods in dentistry and prosthodontics.

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“…15 Whereas mSLA printing has traditionally been relegated to hobbyists requiring low-cost, smaller footprint, but not necessarily high-resolution printers, these recent improvements in LCD technology have leveled the playing field in print quality for mSLA and DLP-SLA printers. Although there are reports of fabrication of molds for microfluidic devices, [16][17][18] and direct 3D printing of microstructures, [19][20][21][22][23] there is limited characterization of the effects of printing parameters like UV power and exposure time on print quality with mSLA printers, and no direct comparisons with DLP-SLA printing. Given that these low-cost mSLA printers are not specifically designed for microfluidics, they also do not have the dedicated documentation and customer service teams to troubleshoot creation of small microfluidic features.…”

Section: Introductionmentioning

confidence: 99%

Democratizing access to microfluidics: Rapid prototyping of capillary microfluidics with a low-cost masked stereolithography 3D printer

Leong,

Quach,

Lin

et al. 2023

Preprint

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Capillary microfluidic devices provide minimally-instrumented and user-friendly liquid handling for a range of biochemical applications. 3D printing is an attractive method for microfabricating capillary microfluidics because it requires little training, is cost-effective compared to conventional cleanroom fabrication, and can drastically decrease prototyping time. High-resolution (≤ 100 μm) microfluidics are typically 3D printed using digital light projection (DLP) stereolithography (SLA); however, the high capital cost (>$10,000) of high-resolution DLP-SLA printers limits their accessibility and widespread use. Recent advances in liquid crystal display (LCD) technology have provided inexpensive (<$500) masked stereolithography (mSLA) 3D printers with sufficient optical resolution for microfluidics. Despite their potential, there have only been a few demonstrations of microfluidic fabrication with mSLA printers, limited characterization of the effects of printing parameters like UV power and exposure time on print quality, and no direct comparisons with DLP-SLA printing. We compared a 40-μm pixel resolution DLP-SLA printer (∼$18,000) with a 34.4-μm pixel resolution (∼$380) mSLA printer. When printing with the same resin and optimized parameters for each printer, the DLP-SLA printer produced features with an average 4.8% difference between designed and measured XY dimensions and a coefficient of variation [CV] = 2.17 ± 2.53% (N=3). The 34.4 μm mSLA printer produced features that were 15.3 ± 11.4% larger than designed dimensions, although they were very precise [CV = 1.80 ± 1.11%, N =3]. Using a low-cost, water-wash resin optimized for the mSLA printer, microchannels printed with the mSLA differed from designed dimensions by only 4.20 ± 4.83%. To demonstrate that we could optimize print conditions for multiple mSLA printers, we printed microchannels with a 22 μm mSLA printer (∼$450) and obtained features that were on average 2.43 ± 5.88% different from designed dimensions. We 3D-printed capillary microfluidic circuits – composed of stop valves, trigger valves, retention burst valves, retention valves, and domino valves – for automating sequential delivery of five liquids. Taken together, our results show that mSLA printers are an inexpensive alternative for fabricating capillary microfluidics with minimal differences in fidelity and accuracy of features compared with a 20X more expensive DLP-SLA printer.

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Digital Light Processing 3D printing for biological applications of polydimethylsiloxane-based microfluidics

Cited by 3 publications

References 51 publications

Advancing 3D printed microfluidics with computational methods for sweat analysis

Advancing 3D printed microfluidics with computational methods for sweat analysis

Mechanical and Material Analysis of 3D-Printed Temporary Materials for Implant Reconstructions—A Pilot Study

Democratizing access to microfluidics: Rapid prototyping of capillary microfluidics with a low-cost masked stereolithography 3D printer

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