Digital in-line holography for biological applications

Abstract

Digital in-line holography with numerical reconstruction has been developed into a new tool, specifically for biological applications, that routinely achieves both lateral and depth resolution, at least at the micron level, in three-dimensional imaging. The experimental and numerical procedures have been incorporated into a program package with a very fast reconstruction algorithm that is now capable of real-time reconstruction. This capability is demonstrated for diverse objects, such as suspension of microspheres and biological samples (diatom, the head of Drosophila melanogaster), and the advantages are discussed by comparing holographic reconstructions with images taken by using conventional compound light microscopy.

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

“…An in-line holographic microscope as developed by the Kreuzer group [16,22,25] was used, consisting of a light source, a pinhole, a wet cell and a detector, all arranged on the same optical axis. For illumination a diode pumped solid state laser providing continuous wave (cw) light with a wavelength of 532 nm with a power of 30 mW was used (IMM Messtechnologie, model GLML4C1-30).…”

Single-pixel digital ghost holography

“…Recording interference patterns with a chargecoupled device (CCD) led to the development of digital holography, where the intensity and phase of the electromagnetic field are measured, stored, transmitted, and manipulated with the aid of a computer [3]. Digital holography is currently a ubiquitous diagnostic and metrological tool [4,5]. Taking biological applications as an example, digital holographic microscopy offers the capability of measuring phase variations in the nanometer range, allowing a marker-free quantitative analysis in the cellular and subcellular ranges [6].…”

Lens-free and portable quantitative phase microscope using a dual-pinhole aperture

“…One representative work is done by H.J. Kreuzer's group [1][2][3][4][5]. Their systems utilize Gabor in-line set-up to trace 4D trajactories of different biological samples with micrometer resolution.…”