Digital in-line holographic microscopy

Garcı́a-Sucerquia, Jorge; Xu, Wenbo; Jericho, S. K.; Klages, P.; Jericho, M. H.; Kreuzer, H. J.

doi:10.1364/ao.45.000836

Cited by 555 publications

(401 citation statements)

References 52 publications

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“…An in-line holographic microscope as developed by the Kreuzer group [16,22,25] was used, consisting of a light source, a pinhole, a wet cell and a detector, all arranged on the same optical axis. For illumination a diode pumped solid state laser providing continuous wave (cw) light with a wavelength of 532 nm with a power of 30 mW was used (IMM Messtechnologie, model GLML4C1-30).…”

Section: Digital In-line Holographic Microscopymentioning

confidence: 99%

“…The wet cell had a volume of 50 ll and was made out of Teflon Ò and sealed with the surfaces of interest. From the recorded holograms, the wavefront was back-propagated through the complete volume via a Kirchhoff-Helmholtz transformation [22]. From such reconstructions, different projections in real space were calculated and spore trajectories were extracted as described previously [14].…”

Section: Digital In-line Holographic Microscopymentioning

confidence: 99%

“…1). Such hologram sequences of moving microscopic objects (down to submicrometer size) provide fourdimensional information (three spatial coordinates and the temporal coordinate) [16,22]. In a previous paper [17] the proof of principle that digital in-line holographic microscopy (DIHM) can be used to capture the motion of zoospores of U. linza, was demonstrated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

et al. 2012

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Settlement of the planktonic dispersal stages of marine organisms is the crucial step for the development of marine biofouling. Four-dimensional holographic tracking reveals the mechanism by which algal spores select surfaces suitable for colonization. Quantitative analysis of the three dimensional swimming trajectories of motile spores of a macroalga (Ulva linza) in the vicinity of surfaces functionalized with different chemistries reveals that their search strategy and swimming behavior is correlated to the number of settled spores found in spore settlement bioassays conducted over 45 min. The spore motility and exploration behavior can be classified into different motion patterns, with their relative occurrence changing with the surface chemistry. Based on the detailed motility analysis we derived a model for the surface selection and settlement process of Ulva zoospores.

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Section: Digital In-line Holographic Microscopymentioning

confidence: 99%

Section: Digital In-line Holographic Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

et al. 2012

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“…A series of such holograms, a holographic movie, is recorded using a single digital detector (camera). From these holograms, three-dimensional information can be retrieved by digital reconstruction algorithms [54,60]. Image analysis of these Recently, an extensive digital in-line holographic microscopy study on Ulva spores by Heydt et al [61] revealed the mechanism by which spores select surfaces suited for settlement.…”

Section: Biofouling Research: the Quest For Environmentallymentioning

confidence: 99%

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Rosenhahn

Sendra

2012

Biointerphases

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This review article summarizes some recent insights into the strategies used by marine organisms to select surfaces for colonization. While larger organisms rely on their sensory machinery to select surfaces, smaller microorganisms developed less complex but still effective ways to probe interfaces. Two examples, zoospores of algae and barnacle larvae, are discussed and both appear to have build-in test mechanisms to distinguish surfaces with different physicochemical properties. Some systematic studies on the influence of surface cues on exploration, settlement and adhesion are summarized. The intriguing notion that surface colonization resembles a parallelized surface sensing event is discussed towards its complementarity with conventional surface analytical tools. The strategy to populate only selected surfaces seems advantageous as waves, currents and storms constantly challenge adherent soft and hard fouling organism.

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“…Lensless microscopy is an established method to locate and estimate the size of micrometric objects in a volume [1,2]. This technique is used in many areas including the study of fluid flows [3,4] and biomedical imaging [5,6].…”

Section: Introductionmentioning

confidence: 99%

Self-calibration for lensless color microscopy

et al. 2017

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Lensless color microscopy (also called in-line digital color holography) is a recent quantitative 3D imaging method used in several areas including biomedical imaging and microfluidics. By targetting cost effective and compact designs, the wavelength of the low-end sources used is only imprecisely known, in particular because of their temperature and power supply voltage dependence. This imprecision is the source of biases during the reconstruction step. An additional source of error is the crosstalk phenomenon, i.e., the mixture in color sensors of signals originating from different color channels. We propose to use a parametric inverse problem approach to achieve the self-calibration of a digital color holographic setup. This process provides an estimation of the central wavelengths and of the crosstalk. We show that taking the crosstalk phenomenon into account in the reconstruction step improves its accuracy.

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Digital in-line holographic microscopy

Cited by 555 publications

References 52 publications

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

Settlement Behavior of Zoospores of Ulva linza During Surface Selection Studied by Digital Holographic Microscopy

Surface Sensing and Settlement Strategies of Marine Biofouling Organisms

Self-calibration for lensless color microscopy

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