Digital Image Analysis of Lymphocyte Activation

Characterization of dehydropeptidase I in the rat lung

“…The physiological significance of the pulmonary dehydropeptidase I on the metabolism of glutathione and its adducts is discussed.The degradation of reduced and oxidized glutathione and of glutathione adducts is initiated by y-glutamyl transferase [l , 21, which removes the y-glutamyl moiety to form L-cysteinylglycine(L-Cys-Gly), L-cystinyl-bis(g1ycine) (~-m y s -G l y J and S (substituent)-L-Cys-Gly adducts, respectively. These dipeptides or dipeptide adducts are further hydrolyzed by dipeptidases or by aminopeptidase M [3,4].Campbell et al purified the dipeptidase hydrolyzing glycyl-dehydrophenylalanine from porcine [S -71 and human [8] kidneys. Recently, it has been reported that this enzyme specifically hydrolyzes the dehydropeptide bond in carbapenem antibiotics, and it has been referred to as dehydrope tiddse I (DHP-I) [9, 101.…”

“…The degradation of reduced and oxidized glutathione and of glutathione adducts is initiated by y-glutamyl transferase [l , 21, which removes the y-glutamyl moiety to form L-cysteinylglycine(L-Cys-Gly), L-cystinyl-bis(g1ycine) (~-m y s -G l y J and S (substituent)-L-Cys-Gly adducts, respectively. These dipeptides or dipeptide adducts are further hydrolyzed by dipeptidases or by aminopeptidase M [3,4].…”