Digital holographic microscopy through a Mirau interferometric objective

León-Rodríguez, Miguel; Rodrı́guez-Vera, Ramón; Rayas, J. A.; Calixto, Sergio

doi:10.1016/j.optlaseng.2012.10.006

Cited by 17 publications

(9 citation statements)

References 18 publications

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“…Over the past decades, thanks to newly available CCD cameras, high performance of computers, and advances in offline numerical processing techniques, DHM has been fully developed and successfully applied in diverse applications; for instance, in quantitative biology microscopy [2][3][4][5][6][7], surface relief characterization of MEMS or other microstructures [8][9][10][11][12], topography of micro-optics [13][14][15][16], and so on [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Guo¹,

Gao²,

Min³

et al. 2013

Appl. Opt.

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A reflection mode digital holographic microscope with light emitting diode (LED) illumination and off-axis interferometry is proposed. The setup is comprised of a Linnik interferometer and a grating-based 4f imaging unit. Both object and reference waves travel coaxially and are split into multiple diffraction orders in the Fourier plane by the grating. The zeroth and first orders are filtered by a polarizing array to select orthogonally polarized object waves and reference waves. Subsequently, the object and reference waves are combined again in the output plane of the 4f system, and then the hologram with uniform contrast over the entire field of view can be acquired with the aid of a polarizer. The one-shot nature in the off-axis configuration enables an interferometric recording time on a millisecond scale. The validity of the proposed setup is illustrated by imaging nanostructured substrates, and the experimental results demonstrate that the phase noise is reduced drastically by an order of 68% when compared to a He-Ne laser-based result.

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Section: Introductionmentioning

confidence: 99%

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Guo¹,

Gao²,

Min³

et al. 2013

Appl. Opt.

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“…Our proposal produces better measurement accuracy due to extended DOF and the numerical reconstruction techniques. With these results, we have demonstrated that it is feasible to perform DHM with the MIO [18].…”

Section: Holographic Materials and Optical Systemsmentioning

confidence: 62%

Microtopography and Thickness Measurement with Digital Holographic Microscopy Highlighting and Its Tomographic Capacity

León-Rodríguez¹,

Rayas-Alvarez²,

García³

et al. 2017

Holographic Materials and Optical Systems

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The refocusing capacity is a unique feature of digital holography. In this chapter, we show the capability of reconstructing digital holograms at different planes for different purposes. One of such purposes is to increase the focus depth of the microscope system. First, we show experimental results of the feasibility to perform digital holographic microscopy (DHM) using a Mirau interferometric objective. A profile phase comparison of a 4.2 μm high microlens using interferometry and DHM, extending the depth of focus of the microscope objective as proof of the proposal, is presented. Second, it is also useful in reducing shot noise when using an LED as a light source. In order to attain the reduction noise, we performed an averaging process of phase and amplitude images reconstructed at different reconstruction distances. This reconstruction range is performed within the focus depth of the optical system. We get a reduction of 50% shot noise. Finally, we show a strategy based on this tomographic capability of reducing a ringing effect by using an ideal filter in off-axis digital holography.

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“…In DHM, many different interferometric configurations have been implemented to work in reflection, such as modified Mach-Zehnder [6][7][8][9], Michelson [10][11][12][13], Linnik [14], Twyman-Green [15], Sagnac [16], and common-path interferometers [17][18][19][20][21][22][23], among others. Perhaps the modified Mach-Zehnder and Michelson interferometers are the most common used layouts.…”

Section: Introductionmentioning

confidence: 99%

Single-Element Reflective Digital Holographic Microscopy

2021

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Digital holographic microscopy (DHM) is a well-known microscopy technique using an interferometric architecture for quantitative phase imaging (QPI) and it has been already implemented utilizing a large number of interferometers. Among them, single-element interferometers are of particular interest due to its simplicity, stability, and low cost. Here, we present an extremely simple common-path interferometric layout based on the use of a single one-dimensional diffraction grating for both illuminating the sample in reflection and generating the digital holograms. The technique, named single-element reflective digital holographic microscopy (SER-DHM), enables QPI and topography analysis of reflective/opaque objects using a single-shot operation principle. SER-DHM is experimentally validated involving different reflective samples.

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Digital holographic microscopy through a Mirau interferometric objective

Cited by 17 publications

References 18 publications

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Microtopography and Thickness Measurement with Digital Holographic Microscopy Highlighting and Its Tomographic Capacity

Single-Element Reflective Digital Holographic Microscopy

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