Digital holographic microscopy for detection of Trypanosoma cruzi parasites in fresh blood mounts

Romero, Gladis Graciela; Monaldi, Andrea Carolina; Alanís, Elvio

doi:10.1016/j.optcom.2011.11.077

Cited by 13 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…6 Quantitative phase imaging is easily facilitated by digital holographic microscopy and has been used to quantify the shape, volume, movement, membrane tension, phase, and other biophysical parameters of erythrocytes, [37][38][39] yeast, 40 myoblasts, 41 neural cells, 42 and trypanosomes. 43 In this paper, we demonstrate a digital holographic microscope (DHM) capable of operating in inline and offaxis modes, between which the user can conveniently switch. Recording hardware has been optimized for high-speed video acquisition of up to 2000 frames per second (fps).…”

Section: Articlementioning

confidence: 99%

A multi-mode digital holographic microscope

Flewellen

Zaid

Berry

2019

Review of Scientific Instruments

View full text Add to dashboard Cite

We present a transmission-mode digital holographic microscope that can switch easily between three different imaging modes: inline, dark field off-axis, and bright field off-axis. Our instrument can be used: to track through time in three dimensions microscopic dielectric objects, such as motile micro-organisms; localize brightly scattering nanoparticles, which cannot be seen under conventional bright field illumination; and recover topographic information and measure the refractive index and dry mass of samples via quantitative phase recovery. Holograms are captured on a digital camera capable of high-speed video recording of up to 2000 frames per second. The inline mode of operation can be easily configurable to a large range of magnifications. We demonstrate the efficacy of the inline mode in tracking motile bacteria in three dimensions in a 160 µm × 160 µm × 100 µm volume at 45× magnification. Through the use of a novel physical mask in a conjugate Fourier plane in the imaging path, we use our microscope for high magnification, dark field off-axis holography, demonstrated by localizing 100 nm gold nanoparticles at 225× magnification up to at least 16 µm from the imaging plane. Finally, the bright field off-axis mode facilitates quantitative phase microscopy, which we employ to measure the refractive index of a standard resolution test target and to measure the dry mass of human erythrocytes.

show abstract

Section: Articlementioning

confidence: 99%

A multi-mode digital holographic microscope

Flewellen

Zaid

Berry

2019

Review of Scientific Instruments

View full text Add to dashboard Cite

show abstract

“…Holographic microscopy has proven to be a versatile and powerful tool for investigating the dynamics of swimming microorganisms at high speed and diffraction-limited resolution ( Katz and Sheng, 2010 ; Romero et al, 2012 ; Weiße et al, 2020 ; Heddergott et al, 2012 ; Wilson et al, 2013 ; Molaei et al, 2014 ; Jikeli et al, 2015 ; Zhang et al, 2018 ; Thornton et al, 2020 ). Three-dimensional tracking, using holographic microscopy or other techniques, unambiguously resolves chirality in the shape of objects and in the geometry of swimming paths.…”

Section: Introductionmentioning

confidence: 99%

High-speed, three-dimensional imaging reveals chemotactic behaviour specific to human-infective Leishmania parasites

Findlay

Osman

Spence

et al. 2021

eLife

View full text Add to dashboard Cite

Cellular motility is an ancient eukaryotic trait, ubiquitous across phyla with roles in predator avoidance, resource access, and competition. Flagellar motility is seen in various parasitic protozoans, and morphological changes in flagella during the parasite life cycle have been observed. We studied the impact of these changes on motility across life cycle stages, and how such changes might serve to facilitate human infection. We used holographic microscopy to image swimming cells of different Leishmania mexicana life cycle stages in three dimensions. We find that the human-infective (metacyclic promastigote) forms display ‘run and tumble’ behaviour in the absence of stimulus, reminiscent of bacterial motion, and that they specifically modify swimming direction and speed to target host immune cells in response to a macrophage-derived stimulus. Non-infective (procyclic promastigote) cells swim more slowly, along meandering helical paths. These findings demonstrate adaptation of swimming phenotype and chemotaxis towards human cells.

show abstract

“…The utility of DHM for detection of microorganisms (especially in sparse environmental samples) has been demonstrated for numerous applications, such as diagnostics, water quality measurements, and ocean monitoring of microbial populations and behavior [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. The technique has also been used to investigate the basic biophysics of microbial motility [ 35 , 36 , 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy

Rouzie

Lindensmith

Nadeau

2021

Life

View full text Add to dashboard Cite

Digital holographic microscopy provides the ability to observe throughout a volume that is large compared to its resolution without the need to actively refocus to capture the entire volume. This enables simultaneous observations of large numbers of small objects within such a volume. We have constructed a microscope that can observe a volume of 0.4 µm × 0.4 µm × 1.0 µm with submicrometer resolution (in xy) and 2 µm resolution (in z) for observation of microorganisms and minerals in liquid environments on Earth and on potential planetary missions. Because environmental samples are likely to contain mixtures of inorganics and microorganisms of comparable sizes near the resolution limit of the instrument, discrimination between living and non-living objects may be difficult. The active motion of motile organisms can be used to readily distinguish them from non-motile objects (live or inorganic), but additional methods are required to distinguish non-motile organisms and inorganic objects that are of comparable size but different composition and structure. We demonstrate the use of passive motion to make this discrimination by evaluating diffusion and buoyancy characteristics of cells, styrene beads, alumina particles, and gas-filled vesicles of micron scale in the field of view.

show abstract

Digital holographic microscopy for detection of Trypanosoma cruzi parasites in fresh blood mounts

Cited by 13 publications

References 10 publications

A multi-mode digital holographic microscope

A multi-mode digital holographic microscope

High-speed, three-dimensional imaging reveals chemotactic behaviour specific to human-infective Leishmania parasites

Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy

Contact Info

Product

Resources

About