Digital expression profiling of a grape-bud EST collection leads to new insight into molecular events during grape-bud dormancy release

Similar mechanisms might be triggered by alternative external stimuli that induce dormancy release in grape buds

“…Mazzitelli et al (2007) also presented Wndings supporting enhanced import of sugars that support the data documented in the current study. Reduction in the level of dehydrin transcript and induction of members of the ubiquitination machinery, calmodulin--regulated ion channel, ATPase, GTP-binding proteins and SNF-related kinase also resemble changes that we detected during grape bud dormancy release (Or et al 2000b;Keilin et al 2007;Pang et al 2007). These similarities may suggest similar cellular processes during dormancy release in woody buds from diVerent species specious in response to diVerent stimulinatural and artiWcial.…”

“…These studies suggest involvement of regulation of gene expression, signaling mechanisms, cell to cell communication and cell cycle in dormancy release (Arora et al 2003;Pang et al 2007;Rohde and Bhalerao 2007). Few large-scale transcription proWling studies, using EST collections and microarrays, may shed light on additional candidate proteins and pathways that might be involved in the regulation and execution of bud dormancy release such as Lea proteins, GTP-binding proteins, Xavonoid biosynthesis and auxin signaling (Pacey-Miller et al 2003;Horvath et al 2005Horvath et al , 2006Keilin et al 2007;Mazzitelli et al 2007;Ruttink et al 2007). Further studies with well deWned experimental systems are needed for the identiWcation of the core cellular processes that are connected with dormancy release.…”

On the mechanism of dormancy release in grapevine buds: a comparative study between hydrogen cyanamide and sodium azide

“…Total RNA hybridization was performed with 32 P-labeled probe for G6PD (TC 46491), amplified from the corresponding pTripleEx2-based clone in our EST collection (Keilin et al 2007), using the insert-screening primers LDF and LDR according to the manufacturer's instructions (Clontech, Palo Alto, CA, USA). Hybridization buffer containing 59 SSC and 109 Denhardt solution was prepared as previously described by Sambrook et al (1989) and hybridization temperature was 55°C.…”

“…Hydrogen cyanamide (HC), commercially applied to overcome dormancy in warm winter regions, provides controlled, synchronized and relatively rapid induction of bud ED release within a well-characterized time frame, thereby creating a traceable and reliable model system for the identification of biochemical pathways involved in the mechanism of bud ED release (Halaly et al 2008;Keilin et al 2007;Nir et al 1986;Nir and Lavee 1993;Or et al 2000Or et al , 2002Pang et al 2007;Pérez and Lira 2005;Pérez et al 2007Pérez et al , 2008. Using this system, catalase expression and activity have been shown to be inhibited shortly after HC application, with the concomitant increase in the level of H 2 O 2 (Nir et al 1986;Nir and Lavee 1993;Or et al 2000Or et al , 2002Pérez and Lira 2005;Pérez et al 2008).…”

“…Using this system, catalase expression and activity have been shown to be inhibited shortly after HC application, with the concomitant increase in the level of H 2 O 2 (Nir et al 1986;Nir and Lavee 1993;Or et al 2000Or et al , 2002Pérez and Lira 2005;Pérez et al 2008). HC applications has also been reported to lead to transcriptional upregulation of GDBRPK, a sucrose non-fermenting protein kinase (SNF-like protein), pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH), thioredoxin h (Trxh), glutathione S-transferase (GST), ascorbate peroxidase (APX), glutathione reductase (GR) and sucrose synthase (SuSy) (Halaly et al 2008;Keilin et al 2007;Or et al 2000;Pérez and Lira 2005;Pérez et al 2008). Overall, these changes suggested the development of respiratory and oxidative stress in the bud as part of the dormancy-release mechanism, and may hint at the mitochondria (Mit) as a potential sensing center for dormancy-release stimuli (Halaly et al 2008;Pérez et al 2007).…”