2023
DOI: 10.1039/d3dd00048f
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Digital biology approach for macroscale studies of biofilm growth and biocide effects with electron microscopy

Konstantin S. Kozlov,
Daniil A. Boiko,
Elena V. Detusheva
et al.

Abstract: Combination of automated scanning electron microscopy and a comprehensive software system that uses deep neural networks to perform an in-depth analysis of biofilms.

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Cited by 3 publications
(3 citation statements)
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References 103 publications
(122 reference statements)
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“…Transforming chemistry through Big Data analytics is an ongoing effort, [31][32][33][34][35][36] but desired ambitious goals are difficult to achieve using single-target/single-objective databases alone. Data fragmentation is a limiting factor that has to be overcome to improve the performance.…”
Section: Big Data and Integrated Datamentioning
confidence: 99%
“…Transforming chemistry through Big Data analytics is an ongoing effort, [31][32][33][34][35][36] but desired ambitious goals are difficult to achieve using single-target/single-objective databases alone. Data fragmentation is a limiting factor that has to be overcome to improve the performance.…”
Section: Big Data and Integrated Datamentioning
confidence: 99%
“…The investigation of biofilm formation under precisely controlled environments, bacteria concentration, and temperature and under various flow regimes was achieved with a versatile microfluidic platform. With the aid of this platform, some features dedicated to biofilm growth were integrated to obtain rapidly defined, reproducible, and localized biofilms with the aim of getting closer to the concept of digital microfluidics for biofilms . With such a tool, traditional static or macroscale flow chamber biofilm studies could be easily transformed, allowing for biofilm culture with lower reagent consumption and higher throughput.…”
Section: Introductionmentioning
confidence: 99%
“…With the aid of this platform, some features dedicated to biofilm growth were integrated to obtain rapidly defined, reproducible, and localized biofilms with the aim of getting closer to the concept of digital microfluidics for biofilms. 42 With such a tool, traditional static or macroscale flow chamber biofilm studies could be easily transformed, allowing for biofilm culture with lower reagent consumption and higher throughput. Moreover, the described method covers some gaps in the existing methods regarding experimental assessment of flows especially regarding the channel’s homogeneity and reproducibility of the biofilm formation.…”
Section: Introductionmentioning
confidence: 99%