Digit specific denervation does not inhibit mouse digit tip regeneration

Dolan, Connor P.; Imholt, Felisha; Yan, Mingquan; Yang, Tae-Jung; Gregory, Joshua A.; Qureshi, Osama; Zimmel, Katherine; Sherman, Kirby M; Smith, Hannah M; Falck, Alyssa; Leininger, Eric; Yu, Ling; Brunauer, Regina; Suva, Larry J.; Gaddy, Dana; Dawson, Lindsay A.; Muneoka, Ken

doi:10.1016/j.ydbio.2022.03.007

Cited by 10 publications

(13 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, anatomical staging of the bone stump is a method to identify the timing of wound closure. Thus, while micro CT analysis has long been utilised to investigate P3 bone regeneration, 11,13–17,22–26,30–34 the current study demonstrates that visualising the bone via micro CT staging also provides a means to infer the state of the radiolucent soft tissue (e.g. cell proliferation and wound closure).…”

Section: Discussionmentioning

confidence: 83%

“…To investigate sequential changes in P3 bone volume, length and morphology, daily microcomputed tomography (micro CT) scans were performed on digits ( n = 9 digits; three mice) from 1 day postamputation (DPA) through a regenerative period of 31 days (Figure 1B–D), and the values were normalised to 1 DPA. Digit regeneration is typically characterised via weekly micro CT analysis, 11,17,23,25,30 yet daily micro CT scanning did not alter the bone regeneration response (Figure S1A, B; t test, 95% CI, p ≥ 0.05, no significance), indicating that the additional radiation dose and/or anaesthesia had no effect. Micro CT analysis reveals that changes in bone volume are minimal between 1 and 4 DPA (Figure 1B, analysis of variance [ANOVA], Tukey's multiple comparisons test, 95% CI, p ≥ 0.05, no significance), consistent with micro CT reconstructed images in which the P3 bone is characterised by a smooth surface from 1 to 3 DPA and minimal pitting at four DPA associated with the onset of the digit osteoclast‐mediated histolytic response (Figure 1D, arrow).…”

Section: Resultsmentioning

confidence: 96%

“…To investigate sequential changes in P3 bone volume, length and morphology, daily microcomputed tomography (micro CT) scans were performed on digits (n = 9 digits; three mice) from 1 day postamputation (DPA) through a regenerative period of 31 days (Figure 1B-D), and the values were normalised to 1 DPA. Digit regeneration is typically characterised via weekly micro CT analysis, 11,17,23,25,30 P3 histolysis is extensive, 14 characterised by osteoclast resorption through the cortex, evident by five DPA, that detaches the distal portion of the stump and exposes the marrow cavity to the wound site (Figure 1D). Stump histolysis culminates in a 40% reduction of bone volume and a 53% reduction of bone length by 10 DPA (Figure 1B, C), thus digit regeneration proceeds from a much smaller structure than the original amputated stump.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Microcomputed tomography staging of bone histolysis in the regenerating mouse digit

Ketcham

Imholt

Yan

et al. 2022

Wound Repair Regeneration

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 96%

“…To investigate sequential changes in P3 bone volume, length and morphology, daily microcomputed tomography (micro CT) scans were performed on digits (n = 9 digits; three mice) from 1 day postamputation (DPA) through a regenerative period of 31 days (Figure 1B-D), and the values were normalised to 1 DPA. Digit regeneration is typically characterised via weekly micro CT analysis, 11,17,23,25,30 P3 histolysis is extensive, 14 characterised by osteoclast resorption through the cortex, evident by five DPA, that detaches the distal portion of the stump and exposes the marrow cavity to the wound site (Figure 1D). Stump histolysis culminates in a 40% reduction of bone volume and a 53% reduction of bone length by 10 DPA (Figure 1B, C), thus digit regeneration proceeds from a much smaller structure than the original amputated stump.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Microcomputed tomography staging of bone histolysis in the regenerating mouse digit

Ketcham

Imholt

Yan

et al. 2022

Wound Repair Regeneration

Self Cite

View full text Add to dashboard Cite

show abstract

“…204,205 Notably, it has been extensively investigated that both mechanical stress and biochemical signals are involved in promoting cell proliferation and renewal induced by exercise. [206][207][208][209][210] Since the complicated mechanism of exercise-induced tissue regeneration advances rapidly, we will discuss in detail the substantial novel progress in the regulation of tissue regeneration from multiple aspects.…”

Section: Molecular Mechanisms Of Tissue Regeneration Induced By Exercisementioning

confidence: 99%

Molecular mechanisms of exercise contributing to tissue regeneration

Chen

Zhou

et al. 2022

Sig Transduct Target Ther

View full text Add to dashboard Cite

Physical activity has been known as an essential element to promote human health for centuries. Thus, exercise intervention is encouraged to battle against sedentary lifestyle. Recent rapid advances in molecular biotechnology have demonstrated that both endurance and resistance exercise training, two traditional types of exercise, trigger a series of physiological responses, unraveling the mechanisms of exercise regulating on the human body. Therefore, exercise has been expected as a candidate approach of alleviating a wide range of diseases, such as metabolic diseases, neurodegenerative disorders, tumors, and cardiovascular diseases. In particular, the capacity of exercise to promote tissue regeneration has attracted the attention of many researchers in recent decades. Since most adult human organs have a weak regenerative capacity, it is currently a key challenge in regenerative medicine to improve the efficiency of tissue regeneration. As research progresses, exercise-induced tissue regeneration seems to provide a novel approach for fighting against injury or senescence, establishing strong theoretical basis for more and more “exercise mimetics.” These drugs are acting as the pharmaceutical alternatives of those individuals who cannot experience the benefits of exercise. Here, we comprehensively provide a description of the benefits of exercise on tissue regeneration in diverse organs, mainly focusing on musculoskeletal system, cardiovascular system, and nervous system. We also discuss the underlying molecular mechanisms associated with the regenerative effects of exercise and emerging therapeutic exercise mimetics for regeneration, as well as the associated opportunities and challenges. We aim to describe an integrated perspective on the current advances of distinct physiological mechanisms associated with exercise-induced tissue regeneration on various organs and facilitate the development of drugs that mimics the benefits of exercise.

show abstract

“…If limbs are denervated, blastema growth and consequent regeneration are halted 6 . Interestingly, mouse digit tip regeneration may also be nervedependent, indicating an evolutionarily conserved mechanism behind the re-growth of a mammalian body part [7][8][9][10] .…”

mentioning

confidence: 99%

Altered developmental programs and oriented cell divisions lead to bulky bones during salamander limb regeneration

et al. 2022

View full text Add to dashboard Cite

There are major differences in duration and scale at which limb development and regeneration proceed, raising the question to what extent regeneration is a recapitulation of development. We address this by analyzing skeletal elements using a combination of micro-CT imaging, molecular profiling and clonal cell tracing. We find that, in contrast to development, regenerative skeletal growth is accomplished based entirely on cartilage expansion prior to ossification, not limiting the transversal cartilage expansion and resulting in bulkier skeletal parts. The oriented extension of salamander cartilage and bone appear similar to the development of basicranial synchondroses in mammals, as we found no evidence for cartilage stem cell niches or growth plate-like structures during neither development nor regeneration. Both regenerative and developmental ossification in salamanders start from the cortical bone and proceeds inwards, showing the diversity of schemes for the synchrony of cortical and endochondral ossification among vertebrates.

show abstract

Digit specific denervation does not inhibit mouse digit tip regeneration

Cited by 10 publications

References 56 publications

Microcomputed tomography staging of bone histolysis in the regenerating mouse digit

Microcomputed tomography staging of bone histolysis in the regenerating mouse digit

Molecular mechanisms of exercise contributing to tissue regeneration

Altered developmental programs and oriented cell divisions lead to bulky bones during salamander limb regeneration

Contact Info

Product

Resources

About