Digestive proteinases in the Japanese spiny lobster Panulirus japonicus

Galgani, François; Nagayama, Fumio

doi:10.1016/0305-0491(87)90409-3

Cited by 28 publications

(20 citation statements)

References 15 publications

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“…fish, squid and soybean meals) in dry food. This conflicts with the fact that they are equipped with a wide repertoire of digestive enzymes, especially proteases (Galgani and Nagayama, 1987;Celis-Gerrero et al,transcriptional regulation of the major isoform appears to occur by a distinct mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…Some information is available on the biochemical characterization of spiny lobster trypsins (Galgani and Nagayama, 1987;Celis-Gerrero et al, 2004;Perera et al, 2008a) and variation in trypsin activity throughout development and molt stages has also been reported (Johnston, 2003;Perera et al, 2008b). However, the effect of diet on these enzymes has been poorly studied and the time course of trypsin activity after ingestion of different diets has only recently been reported for one spiny lobster species (Jasus edwardsii) (Simon, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Dietary protein quality differentially regulates trypsin enzymes at the secretion and transcription level in Panulirus argus by distinct signaling pathways

Perera

Rodríguez-Viera

Rodríguez-Casariego

et al. 2012

Journal of Experimental Biology

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with the results of previous digestibility studies (Ward et al., 2003; Irving and Williams, 2007;Perera et al., 2010b). Poor protein use could be related to some impairment in digestive progression when lobsters are fed on pelleted diets (Simon and Jeffs, 2008;Simon, 2009).The aim of the present work was to assess the effect of feeding P. argus with different protein sources on trypsin expression and secretion. Our results demonstrate that trypsin enzymes are differentially regulated at the transcription and secretion level by ingested proteins. Additionally, we provide evidence that intact proteins, rather than small peptides or free amino acids, are the major signal eliciting the prandial secretory response in lobster, whereas SUMMARYThe effects of pelleted diets with different protein composition (fish, squid or soybean meals as main protein sources) on trypsin secretion and expression were studied in the lobster Panulirus argus. Trypsin secretion was shown to be maximal 4h after ingestion. At this time, fish-and squid-based diets induced trypsin secretion, as well as up-regulation of the major trypsin isoform at the transcription level. While fish-and squid-based diets elicited a prandial response, soybean-based diet failed to stimulate the digestive gland to secrete trypsin into the gastric fluid or induce trypsin expression above the levels observed in fasting lobsters. In vitro assays showed that intact proteins rather than protein hydrolysates stimulate trypsin secretion in the lobster. However, the signal for trypsin transcription appears to be different to that for secretion and is probably mediated by the appearance of free amino acids in the digestive gland, suggesting a stepwise regulation of trypsin enzymes during digestion. We conclude that trypsin enzymes in P. argus are regulated at the transcription and secretion level by the quality of dietary proteins through two distinct signaling pathways. Our results indicate that protein digestion efficiency in spiny lobsters can be improved by selecting appropriated protein sources. However, other factors like the poor solubility of dietary proteins in dry diets could hamper further enhancement of digestion efficiency.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary protein quality differentially regulates trypsin enzymes at the secretion and transcription level in Panulirus argus by distinct signaling pathways

Perera

Rodríguez-Viera

Rodríguez-Casariego

et al. 2012

Journal of Experimental Biology

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“…In Marsupenaeus japonicus, trypsin purified by affinity chromatography has a molecular mass of 25 kDa when analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (Galgani et al, 1985), whereas in Panulirus japonicus (Von Siebold, 1824), Galgani & Nagayama (1987) found trypsin-like activity in a peak of proteolytic activity obtained by gel filtration, with a molecular mass of 24 kDa.…”

Section: Proteinases and Peptidasesmentioning

confidence: 98%

“…In the latter, the study has been based on the use of specific synthetic substrates for each one (Tsai et al, 1986a;Galgani & Nagayama, 1987;Vega-Villasante et al, 1995).…”

Section: Proteinases and Peptidasesmentioning

confidence: 99%

A review of digestive enzyme activity in penaeid shrimps

Carrillo-Farnés¹,

Vega-Villasante²,

Forrellat-Barrios³

et al. 2007

Crustac

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The purpose of this article is to present an overview of the digestive enzymes of penaeid shrimp. The review also covers the effects of circadian rhythms and variations according to moulting cycles on the activities of the various digestive enzymes. RESUMENEl objetivo de este artículo es presentar un panorama de las enzimas digestivas de los camarones peneidos. Esta revisión cubre también aspectos sobre el efecto de las variaciones circadianas y del ciclo de la muda en las actividades enzimáticas.

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“…Highly active proteolytic enzymes acting on alimentary proteins have been reported in many marine species (Celis-Guerrero, Garcìa-Carreño, & Navarrete del Toro, 2004;Muhlia-Almazán, Sánchez-Paz, & García-Carreño, 2008;Wu, Jiang, Xiang, Yang, & Wang, 2008). Proteolytic enzymes in the digestive organs of crustaceans have been well documented and characterized (Dall & Moriarty, 1983;Galgani & Nagayama, 1987;Fang & Lee, 1992;Garcìa-Carreño, 1992;Garcìa-Carreño, Hernandez-Cortes, & Haard, 1994;Glass & Stark, 1994;Jones, Kumlu, Le Vay, & Fletcher, 1997;Navarrete del Toro, García-Carreño, Díaz, Celis-Guerrero, & Saborowski, 2006). Marine decapod crustaceans synthesize a wide range of highly active proteolytic enzymes in the digestive gland: endopeptidases (trypsin and chymotrypsin) and exopeptidases (carboxypeptidases and aminopeptidases).…”

Section: Introductionmentioning

confidence: 99%