Digestive Property and Bioactivity of Blackberry Polysaccharides with Different Molecular Weights

Dou, Zuman; Chen, Chun; Fu, Xiong

doi:10.1021/acs.jafc.9b03505

Cited by 55 publications

(26 citation statements)

References 43 publications

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“…Based on the results of the immunomodulatory activity study, the larger the molecular weight of polysaccharides, the better the immunostimulatory effect on macrophages. According to the relevant literature, 36,37 we found that this may be due to the different molecular weights of PSP-1 and PSP-2, leading to different effects on macrophages. Studies have reported that Lycium barbarum polysaccharides with larger molecular weights had stronger immunomodulatory activity.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Purification, Characterization, and Immunomodulatory Activity Analysis of α-Glucans from Spirulina platensis

Zhang

Shen

et al. 2021

ACS Omega

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Crude polysaccharides from Spirulina platensis (SP) were isolated by maceration with a hot alkali solution and further fractionated by DEAE-52 cellulose and Sephadex G-100 chromatography into two purified fractions PSP-1 and PSP-2. The monosaccharide composition analysis indicated that SP was mainly composed of rhamnose and glucose, while PSP-1 and PSP-2 were composed only of glucose. The composition analysis of PSP-1 and PSP-2 by HPLC, FT-IR, and NMR showed that PSP-1 and PSP-2 were branching dextran, and their structures were (1 → 4)-linked-α-D-Glcp as the main chain, and C-6 replaced the single α-D-Glcp as the linear structure of the branch chain. The glucans (SP/PSP-1/PSP-2) can significantly improve the phagocytic ability of macrophages, enhance iNOS activity, promote NO production, and increase IL-6 mRNA expression, so they may possess certain immunomodulatory activity.

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Section: Resultsmentioning

confidence: 99%

Isolation, Purification, Characterization, and Immunomodulatory Activity Analysis of α-Glucans from Spirulina platensis

Zhang

Shen

et al. 2021

ACS Omega

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“…Furthermore, Sun et al (2018) extracted polysaccharides from Laminaria japonica and found that polysaccharides with smaller molecular weight had a higher antioxidant activity. Meanwhile, Dou et al (2019) discovered that the lower molecular weight blackberry polysaccharide had a stronger bile acid-binding ability and was more easily used by intestinal bacteria.…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells

Pei

Yang

Xiao

et al. 2021

Front. Bioeng. Biotechnol.

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Gelidium crinale, the red algae belonging to Geliaceae Gelidium, is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from Gelidium crinale by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular weight of GNP was 25.8 kDa and the monosaccharide composition had the highest galactose content and confirmed the presence and content (16.5%) of sulfate by Fourier transform infrared spectroscopy (FT-IR) spectrometry as well as barium chloride-gelatin methods. In addition, the effect of GNP on lipopolysaccharide (LPS)-induced oxidative stress and inflammation in macrophages was also evaluated. The research results showed that GNP had fairly strong scavenging activities on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and had Fe2+-chelating ability in a dose-dependent manner. At the same time, it significantly inhibits the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the production of pro-inflammatory cytokines in RAW 264.7 cells induced by LPS through blocking the mitogen-activated protein kinase (MAPK)/nuclear factor kappa beta (NF-κB) signaling pathway. These results indicate that GNP may be a latent component anti-inflammation in pharmaceutical and functional food industries.

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“…Previous studies have demonstrated that branched polysaccharides and those with higher molecular weight have bigger bile acid-binding capacity due to their higher viscosity. 51–53 In addition, CMPS-80 might increase biosynthesis of unsaturated fatty acids by upregulating hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA (HADHA), acyl-CoA thioesterase (ACOT) 1, 2 and 4, ACOX1, ELOVL fatty acid elongase 5 (ESI S6†). Of note, polyunsaturated fatty acids are negatively correlated with the development of CVD.…”

Section: Discussionmentioning

confidence: 99%

The polysaccharide–peptide complex from mushroom Cordyceps militaris ameliorates atherosclerosis by modulating the lncRNA–miRNA–mRNA axis

Miao

Yin

et al. 2022

Food Funct.

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Digestive Property and Bioactivity of Blackberry Polysaccharides with Different Molecular Weights

Cited by 55 publications

References 43 publications

Isolation, Purification, Characterization, and Immunomodulatory Activity Analysis of α-Glucans from Spirulina platensis

Isolation, Purification, Characterization, and Immunomodulatory Activity Analysis of α-Glucans from Spirulina platensis

Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells

The polysaccharide–peptide complex from mushroom Cordyceps militaris ameliorates atherosclerosis by modulating the lncRNA–miRNA–mRNA axis

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