: Y ucca schidigera was fractionated with butan-1-ol, yielding a butanolextractable (BE) fraction, containing all the in vitro antimicrobial activity, and the aqueous, non-butanol-extractable (NBE) fraction. Four groups of Ðve female rats (12 weeks old) were allowed ad libitum access to diets supplemented with water (control) or 200 mg kg~1 total Y schidigera (TOT) or its fraction equivalent of NBE or BE for 64 days. The e †ects of the fractions and their interactions in the TOT treatment were analysed according to the factorial experimental structure by two-way ANOVA. NBE reduced serum urea ([50%, P \ 0É019) and ammonia ([46%, P \ 0É037) concentrations, serum/urine concentration quotients of urea ([79%, P \ 0É009) and ammonia ([57%, P \ 0É002). NBE also reduced hindgut acetate/propionate ([12%, P \ 0É007) but increased faecal ammonia concentration (]87%, P \ 0É039). BE reduced hindgut indoles ([25%, P \ 0É023) and interacted synergystically with NBE in the TOT treatment to further reduce hindgut acetate/propionate by 6% (P \ 0É006). NBE increased (]27%, P \ 0É002) and BE decreased ([57%, P \ 0É005) hindgut urease activity levels, resulting in essentially no change (]4%) in the TOT treatment. The in vitro antimicrobial activity of Y schidigera is an unlikely explanation for most of its e †ects in vivo because these are caused by NBE and in vitro antimicrobial activity is exclusive to BE. Sarsasapogenin and smilagenin were also exclusive ([98%) to BE and cannot account for the e †ects of Y schidigera on N metabolism.1998 SCI. ( J Sci Food Agric 76, 91È99 (1998)