Digestive gland microbiome of <i>Pleurobema cordatum</i>: mesocosms induce dysbiosis

Aceves, Alison K; Johnson, Paul D.; Atkinson, Carla L.; Ee, Brian C. van; Bullard, Stephen A.; Arias, Cova R.

doi:10.1093/mollus/eyaa032

Cited by 2 publications

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“…However, just two of the five mussel species showing temporal variability also exhibited significant variation in the inferred bacterial functionality between the two years. While previous studies have identified significant interspecies variation in the microbiomes of freshwater mussel species [ 12 , 13 ], between those sampled in the wild and in captivity [ 15 ], this is the first report of year-to-year variation in the microbiome within freshwater mussels. Temporal analysis of wild animal microbiomes are uncommon, and this represents only the second that documents microbial variation in wild bivalves (16).…”

Section: Discussionmentioning

confidence: 80%

“…While phylosymbiosis, a correlation between host phylogeny and microbiome composition, was not detected on seven species from the Unionidae family ( Amblema plicata , Obliquaria reflexa , and Pleurobema decisum , in addition to the six included in this study), there were significant phylogenetic patterns in the structure of the microbiome [ 12 ]. However, prior studies on the microbiomes of freshwater mussels were based on data from one season of sampling [ 13 , 14 , 15 ], and the microbiome of marine bivalves has been shown to vary temporally [ 16 , 17 , 18 ]. It is unknown how the gut microbiomes of freshwater mussels may vary from year to year, which could be substantial given their long lifespans.…”

Section: Introductionmentioning

confidence: 99%

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Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

et al. 2021

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Freshwater mussels perform essential ecosystem functions, yet we have no information on how their microbiomes fluctuate over time. In this study, we examined temporal variation in the microbiome of six mussel species (Lampsilis ornata, Obovaria unicolor, Elliptio arca, Fusconaia cerina, Cyclonaias asperata, and Tritogonia verrucosa) sampled from the same river in 2016 and 2019. We examined the taxonomic, phylogenetic, and inferred functional (from 16S rRNA sequences) facets of their microbiome diversity. Significant differences between the two years were identified in five of the six species sampled. However, not all species that exhibited a temporally variable microbiome were functionally distinct across years, indicating functional redundancy within the mussel gut microbiome. Inferred biosynthesis pathways showed temporal variation in pathways involved in degradation, while pathways involved in cellular metabolism were stable. There was no evidence for phylosymbiosis across any facet of microbiome biodiversity. These results indicate that temporal variation is an important factor in the assembly of the gut microbiomes of freshwater mussels and provides further support that the mussel gut microbiome is involved in host development and activity.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

et al. 2021

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“…Alphaproteobacteria were more evenly distributed, comprising an average of 10.3% and 9.9% of reads in cases and controls, respectively. A previous study found strong shifts in unionid gut microbiome composition when comparing wild Ohio pigtoe ( Pleurobema cordatum ) to individuals held in mesocosms under conditions known to induce wasting syndromes and mortality, with shifts to dominance by Proteobacteria in the latter [ 34 ]. Proteobacteria were also the most abundant taxa in the gut bacterial communities of the marine bivalve P. nobilis during a mass mortality event in the Mediterranean Sea [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recent work for both marine and freshwater bivalves has characterized microbiomes using 16S rRNA metabarcoding to explore relationships among bivalves, their associated bacteria, and the environment. Freshwater mussel gut microbiomes differ in wild and captive habitats [ 33 , 34 ], are functionally stable [ 35 ], are determined by host species, and are selectively retained from filtered seston [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Mussel Mass Mortality and the Microbiome: Evidence for Shifts in the Bacterial Microbiome of a Declining Freshwater Bivalve

et al. 2021

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Freshwater mussels (Unionida) are suffering mass mortality events worldwide, but the causes remain enigmatic. Here, we describe an analysis of bacterial loads, community structure, and inferred metabolic pathways in the hemolymph of pheasantshells (Actinonaias pectorosa) from the Clinch River, USA, during a multi-year mass mortality event. Bacterial loads were approximately 2 logs higher in moribund mussels (cases) than in apparently healthy mussels (controls). Bacterial communities also differed between cases and controls, with fewer sequence variants (SVs) and higher relative abundances of the proteobacteria Yokenella regensburgei and Aeromonas salmonicida in cases than in controls. Inferred bacterial metabolic pathways demonstrated a predominance of degradation, utilization, and assimilation pathways in cases and a predominance of biosynthesis pathways in controls. Only two SVs correlated with Clinch densovirus 1, a virus previously shown to be strongly associated with morality in this system: Deinococcota and Actinobacteriota, which were associated with densovirus-positive and densovirus-negative mussels, respectively. Overall, our results suggest that bacterial invasion and shifts in the bacterial microbiome during unionid mass mortality events may result from primary insults such as viral infection or environmental stressors. If so, bacterial communities in mussel hemolymph may be sensitive, if generalized, indicators of declining mussel health.

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Digestive gland microbiome of Pleurobema cordatum: mesocosms induce dysbiosis

Cited by 2 publications

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Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

Gut Microbiomes of Freshwater Mussels (Unionidae) Are Taxonomically and Phylogenetically Variable across Years but Remain Functionally Stable

Mussel Mass Mortality and the Microbiome: Evidence for Shifts in the Bacterial Microbiome of a Declining Freshwater Bivalve

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