Digestive enzyme activity in juvenile Nile tilapia (Oreochromis niloticus, L) submitted to different dietary levels of shrimp protein hydrolysate

Abstract

The effect of different dietary concentrations of shrimp protein hydrolysate (SPH) on digestive enzyme activity of Nile tilapia juveniles was evaluated. SPH concentrations in diets were 0, 15, 30 and 60 g kg-1 (treatments SPH0, SPH15, SPH30 and SPH60, respectively). Hemoglobin, azocasein, BApNA (Na-benzoyl-DL-arginine-p-nitroanilide), SApNA (Suc-Ala-Ala-Pro-Phe p-nitroanilide), aminoacyl of b-naphthylamide and starch were used as substrates for enzyme activity determinations. The activity of total alkaline protease was significantly higher (P \ 0.05) in fish under SPH15 and SPH60 treatments than in the control (SPH0). However, the effect was not dosedependent. Substrate-SDS-PAGE was also performed to evaluate changes in the profile

Dietary protein:lipid ratio changes growth, digestive enzyme activity, metabolic profile and haematological parameters in hybrid surubim (Pseudoplatystoma fasciatum × Leiarius marmoratus)

“…Nutrients from feed are only bioavailable for growth and metabolism after the digestion process is complete. Protein, carbohydrate and lipid concentration from the diet might increase or decrease digestive enzyme activity (Li et al., ; Pérez‐Jimenes et al., ; Santos et al., ). Among the proteases evaluated in this study, chymotrypsin was the only one that showed a significant difference.…”

“…The resulting solution was centrifuged at 10,000 g for 10 min at 4°C. The supernatants (crude enzyme extract) were frozen at À20°C and used for analysis assays (Santos et al, 2013). Protein concentration was determined according to Bradford (1976) using standard BSA (bovine serum albumin).…”

Comparison of hydrolysed proteins from different raw materials in diets for Mozambique tilapia Oreochromis mossambicus

“…Tilapias are a globally important group of aquaculture species, and significant amounts of fishmeal are used to produce formulated feeds for them (Bostock et al 2010;FAO 2010). As in other finfish, FPH products have been evaluated as protein source and potential replacers of fishmeal (Cavalheiro et al 2007;El-Hakim et al 2007;Fagbenro et al 1994;Leal et al 2010;Plascencia-Jatomea et al 2002) and soybean meal (Hernández et al 2013), as immune stimulant (Goosen et al 2014a) or to determine the effects of FPH inclusion on intestinal digestive enzyme activity (Santos et al 2013). In fishmeal replacement studies, it was generally found that FPH can replace a portion of the fishmeal in formulated tilapia diets without negatively impacting production performance.…”

The effects of pre-digested protein sources on the performance of early–mid stage Panulirus ornatus phyllosoma

“…Therefore, since growth is primarily an increase in muscle mass by protein synthesis and accretion (Anger, 2001), the high growth rate of the HFM fed phyllosoma can be attributed to the increased capacity of the gut system to rapidly provide the body with protein precursors, namely peptides and amino acids (Finn et al, 2002;Rønnestad et al, 2003;Tonheim et al, 2005;Zhang et al, 2006). However, the extent of hydrolysis performed on intact protein can determine the quality of the resultant diet, since different contents of amino acids and peptide chain-lengths have the capacity to substantially alter results (Santos et al, 2012). In the present study, the extent of the enzyme hydrolysis process was performed so as to cleave peptide bonds to form smaller chain sizes, without actually releasing an abundance of FAAs.…”