Digestive Enzyme Activities in Barred Loach (Nemacheilus fasciatus, Val., 1846.): Effect of pH and Temperature

Abstract

This study aims to determine the total protease, lipase, and amylase activities at different pHs, as well as pepsin and trypsin-like at different temperatures. A total of 240 individuals have been used in this study. Enzyme activity was measured by the spectrophotometer method. The effect of pH was evaluated on protease, lipase, and amylase activity, while the effect of temperature was evaluated on pepsin and trypsin-like activities.The results showed that the total protease activity at pH 7.0-10.0 was significantly higher than pH 1.7-5.0 (P <0.05). Furthermore, the activity of lipase was significantly higher at pH 5.0-7.0 than pH 1.7, 3.4, and 10.0. Also, the activity of amylase at pH 7.0-8.0 was significantly higher (p <0.05) than pH 1.7-5.0 and pH 10.0. Moreover, the pepsin-like activity in the anterior gut was significantly higher (p <0.05) than the posterior gut.Conversely, trypsin-like activity in the posterior gut was significantly higher (p <.05) than the anterior gut. Additionally, the pepsin-like activity was significantly higher at 45°C compared to different temperatures (p <0.05), whereas trypsin-like was significantly (p <0.05) higher at 60 °C than othertemperatures. Conclusively, the total protease and amylase activity was higher under neutral to slightly alkaline conditions, while lipase was higher under acidic to neutral conditions. Furthermore, the pepsin-like activity was only found in the anterior gut, whereas trypsin-like was higher in the posterior gut. The optimal temperature for pepsin-like and trypsin-like activity was 45 °C and 60 °C, respectively.

Efek Perbedaan Temperatur terhadap Aktivitas Tripsin-Like dan Amilase Digesti Ikan Lunjar (Rasbora lateristriata Blkr.)

“…Metode Folin-Cioclateu's dengan kasein sebagai substrat telah digunakan untuk mengukur aktivitas tripsin-like (Susilo et al, 2022). Larutan buffer Tris-HCl 0,1 M (pH 7,6) telah digunakan untuk mengukur aktivitas tripsin-like.…”

“…pada penelitian ini, ternyata tidak berbeda dengan penelitian sebelumnya pada ikan Pangasianodon gigas, Luphiosilurus alexadri, Catla catla, dan Cirrhinus mrigala yang memperlihatkan penurunan aktivitas tripsin pada temperatur 60-70°C (Vannabun et al, 2014;Kangembam & Chakrabarti, 2015;Santos et al, 2016;Dhara et al, 2017). Hal yang tidak berbeda juga dijumpai pada ikan Nemacheilus fasciatus yang mengalami penurunan aktivitas tripsin-like pada temperatur 75ºC (Susilo et al, 2022). Namun hasil penelitian ini berbeda dengan penelitian sebelumnya yang dilakukan pada Sardinella longiceps dan Scorpaena notata yang mencapai aktivitas maksimal tripsin pada temperatur 40-45°C (Aissaoui et al, 2017;Khandagale et al, 2017), sedangkan pada ikan Cyclochellichthys apogon dan Amblypharyngodon mola aktivitas maksimal tripsin dicapai pada temperatur 60°C (Champasri & Champasri, 2017;Debnath et al, 2020).…”

Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application

“…Developments in the domestication of barred loach are necessary to raise their number in the wild, e.g. through supplemental stocking, and meet the demands of the community in terms of fisheries and aquarium trade (Susilo et al, 2022).…”