Digestive cell turnover in digestive gland epithelium of slugs experimentally exposed to a mixture of cadmium and kerosene

Zaldibar, Beñat; Cancio, Ibon; Soto, Manu; Marigómez, Ionan

doi:10.1016/j.chemosphere.2007.06.071

Cited by 34 publications

(20 citation statements)

References 33 publications

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“…Increasing rates of cell proliferation within the digestive gland upon exposure to contaminants have also been observed in other gastropod species (Zaldibar et al 2007a, b) and seem to be related to changes in cell type composition and cell renewal in the gastropod midgut gland (Zaldibar et al 2008). Several studies suggest that, upon metal pollution or contaminant exposure, digestive cell numbers in the midgut gland of pulmonate snails decrease (Marigomez et al 1996;Zaldibar et al 2007a, b), whereas excretory cell numbers increase (Marigomez et al 1996;Zaldibar et al 2007b). Basophilic cell numbers, on the other hand, seem to increase upon toxicant exposure, at least in proportion to the decreasing numbers of digestive cells (Lowe and Clarke 1989;Marigomez et al 1996).…”

Section: Protective Effect Of Cd-mt Expressionmentioning

confidence: 91%

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

et al. 2010

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In terrestrial pulmonate snails, cadmium (Cd) uptake leads to the induction of a Cd-specific metallothionein isoform (Cd-MT) that protects against adverse interactions of this toxic metal ion. Increasing concentrations of Cd cause increased individual mortality possibly linked to pathological alterations in the snail midgut gland. Histological, immuno-histochemical, and electron-microscopic methods in combination with tissue metal analyses and quantification of MT induction parameters were applied to the midgut gland of Cd-exposed Roman snails (Helix pomatia). Conspicuous concentration-dependent alterations occurred in this organ, including the metal-induced increase of Cd-MT concentration and manifestation of Cd-MT mRNA precipitations in all midgut gland cell types. The most evident alteration was an increase of cellular turnover reflected by enhanced cell proliferation. Intensified vesiculation of endoplasmic reticulum was noted in basophilic cells and an increasing formation of lipofuscin granules in excretory cells. At the highest Cd concentrations, mitochondrial membranes were disrupted in basophilic cells, and lipofuscin granules were released from excretory cells into the midgut gland tubular system. Some of these alterations (e.g., increased cell proliferation rate, vesiculation of endoplasmic reticulum) detected at low Cd concentrations were interpreted as adaptive response processes enhancing the tolerance of exposed individuals to metal stress. Cellular alterations at higher Cd concentrations (e.g., mitochondrial structural damage) clearly represented ongoing irreversible cellular disruption. Combined evaluation of cellular biomarkers and MT saturation levels indicated that the transition from stress resistance to depletion of resistance capacity occurred above a threshold of 0.8 micromol Cd/g dry weight in the midgut gland of H. pomatia. At these Cd concentrations, Cd-MT was saturated with Cd(2+) ions, whereas at the cellular level, structural alterations turned into pathological deterioration.

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Section: Protective Effect Of Cd-mt Expressionmentioning

confidence: 91%

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

et al. 2010

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“…Histopathological lesions in bivalves have already been related to soft-tissue concentrations of contaminants (Gold-Bouchot et al 1995). In general, the gills and digestive glands are, in mollusks, the major target organs for pollution studies (Gold-Bouchot et al 1995;Syasina et al 1997;Zaldibar et al 2007Zaldibar et al , 2008. Still, histopathology studies in C. edule are absent.…”

Section: Introductionmentioning

confidence: 96%

Evaluation of the potential of the common cockle (Cerastoderma edule L.) for the ecological risk assessment of estuarine sediments: bioaccumulation and biomarkers

et al. 2010

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Common cockles (Cerastoderma edule, L. 1758, Bivalvia: Cardiidae) were subjected to a laboratory assay with sediments collected from distinct sites of the Sado Estuary (Portugal). Cockles were obtained from a mariculture site of the Sado Estuary and exposed through 28-day, semi-static, assays to sediments collected from three sites of the estuary. Sediments from these sites revealed different physico-chemical properties and levels of metals and organic contaminants, ranging from unimpacted (the reference site) to moderately impacted, when compared to available sediment quality guidelines. Cockles were surveyed for bioaccumulation of trace elements (Ni, Cu, Zn, As, Cd and Pb) and organic contaminants (PAHs, PCBs and DDTs). Two sets of potential biomarkers were employed to assess toxicity: whole-body metallothionein (MT) induction and digestive gland histopathology. The bioaccumulation factor and the biota-to-soil accumulation factor were estimated as ecological indices of exposure to metals and organic compounds. From the results it is inferred that C. edule responds to sediment-bound contamination and might, therefore, be suitable for biomonitoring. The species was found capable to regulate and eliminate both types of contaminants. Still, the sediment contamination levels do not account for all the variation in bioaccumulation and MT levels, which may result from the moderate metal concentrations found in sediments, the species' intrinsic resistance to pollution and from yet unexplained xenobiotic interaction effects.

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“…The mechanism underlying the feeding impairment observed in mollusks exposed to molluscicides is not fully understood, but it could apparently be strongly related to damage to the gastric system or even impacts on the nervous system, as some authors have suggested (Zaldibar et al, 2007). The cellular mechanism of the action of saponins leading to epithelium fragmentation and ulcer- it is important to note that they are all obtained through other sub-processes, resulting in high availability of these saponin products at a low cost.…”

Section: Histology Of the Crop Regionmentioning

confidence: 99%

Molluscicidal effects of saponin-rich plant extracts on the grey field slug

González-Cruz

Martín

2013

Cienc. Inv. Agr.

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D. González-Cruz, and R. San Martín. 2013. Molluscicidal effects of saponin-rich plant extracts on the grey field slug. Cien. Inv. Agr. 40(2): 341-349. Slug control is a major concern in crop fields because these mollusks consume and damage plants that are destined for human consumption and ornamentation. The grey field slug Deroceras reticulatum (Müller, 1774) is one of the most commonly occurring mollusks with agronomic importance, consuming up to 80% of the leaf surface of raps and other crops. The major difficulty in slug control is that the main products used for this purpose can also endanger non-target animals, such as pets and birds. New methods of control that are naturally based and environmental friendly are urgently required, both for the farmer who applies the product and for the final consumer. In this study, we analyzed the anti-molluscal activity of extracts from three plant with a high content of saponins: Camellia oleifera, Gleditsia amorphoides and Quillaja saponaria. Forced oral injection and histological analyses of the digestive system were performed to measure the effects of the plant extracts against slugs. All three extracts showed anti-feeding activity and toxicity against slugs when injected orally at a range of concentrations between 1 and 4% w/w. All three extracts also damaged the gastric epithelium of the crop region of the slugs, suggesting that they show strong membranolytic properties in the digestive system of these mollusks. This study revealed novel natural compounds that can potentially be used in the control of grey field slugs. The data hereby presented could be useful in future comparative studies between other mollusks with agronomic importance.

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Digestive cell turnover in digestive gland epithelium of slugs experimentally exposed to a mixture of cadmium and kerosene

Cited by 34 publications

References 33 publications

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

Evaluation of the potential of the common cockle (Cerastoderma edule L.) for the ecological risk assessment of estuarine sediments: bioaccumulation and biomarkers

Molluscicidal effects of saponin-rich plant extracts on the grey field slug

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