Digestion of soybean globulins, glycinin, α-conglycinin and β-conglycinin in the preruminant and the ruminant calf

Tukur, H. M.; Lallès, JP; Mathis, C.P.; Caugant, I.; Toullec, R.

doi:10.4141/cjas93-091

Cited by 35 publications

(37 citation statements)

References 14 publications

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“…Under our experimental conditions, in which soybean provided between 58 and 71 % of dietary crude protein (CP), the digestibility of soybean N was best predicted by the levels of immunoreactive p-conglycinin, or by the anti-tryptic activity when the products were devoid of (3-conglycinin. Highly antigenic products, such as heated soybean flour, presented the lowest digestibility, which could be partially explained by an increased flow of undigested globulins in the ileum [37]. The intrinsic resistance of legume seed protein to digestion [23] [19].…”

mentioning

confidence: 99%

Morphology and enzyme activities of the small intestine are modulated by dietary protein source in the preruminant calf

Montagné¹,

Toullec²,

Savidge³

et al. 1999

Reprod. Nutr. Dev.

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-A study was undertaken to assess the impact of the protein nature and soya antigenicity on the morphology and some enzyme activities of the jejunum in preruminant calves. Twenty Holstein calves fitted with a duodenal cannula were fed a liquid diet based on skimmed milk powder (SMP) for 2 weeks. They were then switched onto diets containing a mixture of SMP and either antigenic heated soybean flour (HSF; n = 12) or hypo-antigenic soya protein concentrate (SPC; n = 8) for 8 weeks, after which they were reverted back to the SMP diet for 2 weeks. The diets contained similar amounts of digestible nitrogen and energy, and were fed at a rate of 55 g DM/kg o . 75/d. Proximal jejunal biopsies were collected just before (week 0), during (weeks 2 and 8) and after (week 10) feeding of the soya-based diets, and were used for morphology measurements and the determination of total alkaline phosphatase, lactase, amino-peptidases A and N, and dipeptidyl peptidase IV activities. Feed intake and growth were similar between the HSF and SPC groups during the experimental period. The effects of antigenicity and the antigenicity x time interaction were never significant (P > 0.05). Villus height decreased (P < 0.01) between weeks 0 and 2, and increased (P < 0.05) between weeks 8 and 10. Villus width increased between weeks 2 and 8 (P < 0.001 Crypt depth also increased between weeks 0 and 2 (P < 0.001 Specific activities of alkaline phosphatase (P < 0.01) and amino-peptidase N (P < 0.05) decreased between weeks 0 and 2. Conversely, those of alkaline phosphatase (P < 0.0001 ), lactase (P < 0.01) and dipeptidyl-peptidase IV (P < 0.0001) increased between weeks 8 and 10. Specific activities for lactase and amino-peptidase N decreased (P < 0.01) between weeks 2 and 8.

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mentioning

confidence: 99%

Morphology and enzyme activities of the small intestine are modulated by dietary protein source in the preruminant calf

Montagné¹,

Toullec²,

Savidge³

et al. 1999

Reprod. Nutr. Dev.

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“…The antitryptic activity in the soybean flours was analysed according to the method of Liu and Markakis [ 16]. The residual glycinin and P-conglycinin immunoreactivities were determined by dot-blotting [13] using hyperimmune sera directed against the native globulins [26]. The AA concentrations of the soybean flours and casein were determined by ion-exchange chromatography following hydrolysis in 0.5 N HCI for 24 and 48 h [17].…”

mentioning

confidence: 99%

Nutritional value of the proteins of soybeans roasted at a small-scale unit level in Africa as assessed using growing rats

Ouédraogo¹,

Combe²,

Lallès³

et al. 1999

Reprod. Nutr. Dev.

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“…Diets and digesta samples were analysed for DM, nitrogen (N), fat and ash according to methods described previously (Guilloteau et al, 1986 Glycinin and (3-conglycinin immunoreactivities of the soyabean concentrate were assayed according to previously described methods (Tukur et al, 1993). The antitryptic activity of the legume concentrates was determined according to the AOCS methods (1983).…”

Section: Chemical Analysesmentioning

confidence: 99%

“…The antitryptic activity of the legume concentrates was determined according to the AOCS methods (1983). SDS-PAGE electrophoresis (Laemmli, 1970) was carried out under reducing conditions for the 2 legume concentrates and their corresponding raw flours, as well as purified soyabean globulins (Tukur et al, 1993).…”

Section: Chemical Analysesmentioning

confidence: 99%

“…In contrast, soyabean flour and an alcohol-treated concentrate were found to induce larger changes in digesta composition (Guilloteau etal, 1986;Khorasani et al, 1989;Caugant et al, 1993a (Souffrant, 1991) and the direct assay of dietary protein in digesta (Tukur et al, 1993) (Toullec et al, 1979;Beynen and Van Gils, 1983;Nunes do Prado et al, 1989b). Thus despite the high contents of lipids reaching the duodenum, the circulating level of triglycerides remained unchanged or decreased with the control diet.…”

Section: Chemical Analysesmentioning

confidence: 99%

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Digestibility, blood levels of nutrients and skin responses of calves fed soyabean and lupin proteins

Tukur¹,

Pardal²,

Formal³

et al. 1995

Reprod. Nutr. Dev.

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Summary ― Three milk substitute diets in which the protein was provided either by skim milk only (control diet) or mainly (71%) by a commercial soyabean or lupin concentrate (soyabean or lupin diet, respectively) were given to intact or ileo-caecal-cannulated preruminant calves. In vitro tests showed that both concentrates were partially proteolysed and had low antigenic and antitryptic activities. The low antigenicity was confirmed in vivo since none of the calves produced specific plasma antibodies against dietary proteins, and skin reactions following the injection of these proteins were minor. Postprandial plasma level of triglycerides was higher with the 2 legume diets, suggesting faster abomasal emptying of fat and probably protein. Apparent faecal nitrogen digestibility was lower ( P 5 0.05) with the soyabean and lupin diets than with the control diet (0.86, 0.88 and 0.95, respectively). At the ileal level, the differences were smaller and non-significant (0.90, 0.88 and 0.92) for nitrogen, but remained significant for valine and tyrosine with the soyabean diet, and for proline, valine, methionine, leucine and lysine with the lupin diet. However, the differences were small enough to conclude that proper denaturation of soyabean and lupin proteins by processes including partial hydrolysis can suppress their antigenicity and render them very digestible.

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Digestion of soybean globulins, glycinin, α-conglycinin and β-conglycinin in the preruminant and the ruminant calf

Cited by 35 publications

References 14 publications

Morphology and enzyme activities of the small intestine are modulated by dietary protein source in the preruminant calf

Morphology and enzyme activities of the small intestine are modulated by dietary protein source in the preruminant calf

Nutritional value of the proteins of soybeans roasted at a small-scale unit level in Africa as assessed using growing rats

Digestibility, blood levels of nutrients and skin responses of calves fed soyabean and lupin proteins

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