2000
DOI: 10.1046/j.1432-1327.2000.01242.x
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Digestion of single crystals of mannan I by an endo‐mannanase from Trichoderma reesei

Abstract: The enzymatic degradation of single crystals of mannan I with the catalytic core domain of a b-mannanase (EC 3.2.1.78 or Man5A) from Trichoderma reesei was investigated by transmission electron microscopy and electron diffraction. The enzyme attack took place at the edge of the crystals and progressed towards their centres. Quite remarkably the crystalline integrity of the crystals was preserved almost to the end of the digestion process. This behaviour is consistent with an endo-mechanism, where the enzyme in… Show more

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Cited by 11 publications
(11 citation statements)
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“…Enzymatic degradations of single crystals of various samples have been performed. In the field of polysaccharide, single crystals of β (1→4)xylan, nigeran, and β (1→4)mannan have been subjected to degradation by xylanases, mycodextranase, and mannanases, respectively. In the field of biodegradable aliphatic polyesters, various types of extracellular PHB depolymerases have been used to study the degradation of single crystals of poly([ R ]-3-hydroxybutyrate) (P([ R ]-3HB)) and its copolymer as well as poly( β -propiolactone) (PPL) single crystals.…”
Section: Introductionmentioning
confidence: 99%
“…Enzymatic degradations of single crystals of various samples have been performed. In the field of polysaccharide, single crystals of β (1→4)xylan, nigeran, and β (1→4)mannan have been subjected to degradation by xylanases, mycodextranase, and mannanases, respectively. In the field of biodegradable aliphatic polyesters, various types of extracellular PHB depolymerases have been used to study the degradation of single crystals of poly([ R ]-3-hydroxybutyrate) (P([ R ]-3HB)) and its copolymer as well as poly( β -propiolactone) (PPL) single crystals.…”
Section: Introductionmentioning
confidence: 99%
“…Some mannanases are able to hydrolyze not only the ␤-1,4-bond between two mannose units, but also the bond between the adjacent glucose and mannose units (24,25). Interestingly, some mannanases are able to degrade mannan crystals quite efficiently (26,27).…”
Section: Mannanasesmentioning
confidence: 99%
“…The aim of the present study, which follows an earlier preliminary report, was to gain more information on the mode of action of mannan-degrading enzymes on crystalline mannan. For this purpose, mannan crystals were digested by the enzymes and the samples were analyzed for release of reducing sugars.…”
Section: Introductionmentioning
confidence: 98%