Digestion of protein in different marine species

Glass, H.J.; MacDonald, N.L.; Morán, Rolando; Stark, J.R.

doi:10.1016/0305-0491(89)90203-4

Cited by 42 publications

(35 citation statements)

References 11 publications

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“…Effect of pH on the proteolytic activity The purified protease was separately diluted in the following buffers with different pH values: 0.1 M KCl-HCl (pH 1.0-2.0); 0.2 M Gly-HCl (pH 3.0-4.0); 0.1 M citrate acid-citrate sodium (pH 5.0-6.0); 0.1 M Tris-HCl (pH 7.0); 0.2 M NaH 2-PO 4 -Na 2 HPO 4 (pH 8.0); 0.1 M Na 2 CO 3 -NaH-CO 3 -NaOH (pH 9.0-12.0); 0.1 M Gly-NaOH (pH 13.0-14.0) (Glass et al 1989;Munilla-Moran and Rè y 1996). The activity was measured as described above.…”

Section: Characterization Of the Purified Proteasementioning

confidence: 99%

Purification and characterization of a cysteine-like protease from the body wall of the sea cucumber Stichopus japonicus

Dong

Cong

et al. 2007

Fish Physiol Biochem

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The sea cucumber (Stichopus japonicus) is able to undergo autolysis in response to a variety of environmental and mechanical cues. Within the framework of a long-term study of this phenomenon we have purified a protease from the body wall of the sea cucumber by means of ion-exchange chromatography with DE-52 cellulose and gel filtration chromatography with Sephadex G-100. The final enzyme preparation was nearly homogeneous on polyacrylamide gel electrophoresis, and its molecular weight was estimated to be approximately 35.5 kDa. The purified enzyme exhibited a maximum activity for the hydrolysis of casein at pH 7.0 and 50°C and a remarkable stability at pH 4.0-7.0 and 40-60°C. Based on the inhibition and activation profiles obtained with numerous specific protease inhibitors and an activator, the protease purified from the body wall of the sea cucumber was defined to be a cysteine-like protease.

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Section: Characterization Of the Purified Proteasementioning

confidence: 99%

Purification and characterization of a cysteine-like protease from the body wall of the sea cucumber Stichopus japonicus

Dong

Cong

et al. 2007

Fish Physiol Biochem

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“…A series of buffers were used for different pH conditions: 0.1 M KCl-HCl (pH 1.5-2.5), 0.2 M Gly-HCl (pH 3.0-4.0), 0.2 M phosphate buffer (pH 5.0-6.0), 0.1 M Tris-HCl (pH 7.0-9.0) and 0.1 M Gly-NaOH (pH 10.0-14.0) (Glass, MacDonald, Moran, & Stark, 1989;Munilla-Moran & Rey, 1996). Briefly, 0.2 mL protease extract was added to 0.8 mL of the above buffers containing 2 g L À1 azocasein, followed by an incubation at 37°C for 1 h. The reaction was stopped by the addition of 0.5 mL 12% trichloroacetic acid (TCA).…”

Section: Proteolytic Activitymentioning

confidence: 99%

Study of a highly alkaline protease extracted from digestive tract of sea cucumber (Stichopus japonicus)

Xue

Miao

et al. 2005

Food Research International

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“…Physiology and nutrition studies of fish in the early stages of development, as well as the evolution of the digestive enzyme activity, are valuable tools to better know the nutritional capabilities of young larvae and establish feeding protocols for optimizing larval mass rearing production (Glass et al 1989;Ueberschär 1993Ueberschär , 1995Diaz et al 1997;Kolkovski 2001;Furné et al 2005;Suzer et al 2007). Furthermore, the assessment of the presence and level of activity of certain enzymes (e.g.…”

Section: Introductionmentioning

confidence: 98%

Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition

Tong

Shao

Liu

et al. 2011

Fish Physiol Biochem

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Digestive enzyme activities were analysed in turbot (Scophthalmus maximus) from hatching until 60 days after hatching (DAH). Trypsin sharply increased to the climax at 17 DAH and decreased until 31 DAH followed by a stable level thereafter. Amylase was determined at 4 DAH, reached the maximum value at 19 DAH and declined sharply to 39 DAH and remained at a low level thereafter, suggesting the carbohydrate component should remain at a low level in formulated diets. Pepsin was detected at 9 DAH and increased to 34 DAH and then remained at a stable level. The above results revealed pancreatic enzymes are no longer main enzymes for food digestion after the formation of functional stomach. Leucine-alanine peptidase (Leu-ala) and alkaline phosphatase (AP) and leucine aminopeptidase N (LAP) were found in newly hatched larvae. Both AP and LAP activities markedly increased to 23 DAH, decreased abruptly to 50 DAH and increased gradually to 60 DAH. Leu-ala reached the plateau from 23 to 39 DAH, followed by a decline to 46 DAH and an increase until 60 DAH. The brush border membrane (BBM)-bound enzyme activities increased from 30% at 31 DAH to 81% at 38 DAH of the total activities, indicating the maturation of intestinal tract.

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Digestion of protein in different marine species

Cited by 42 publications

References 11 publications

Purification and characterization of a cysteine-like protease from the body wall of the sea cucumber Stichopus japonicus

Purification and characterization of a cysteine-like protease from the body wall of the sea cucumber Stichopus japonicus

Study of a highly alkaline protease extracted from digestive tract of sea cucumber (Stichopus japonicus)

Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition

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