Digestion of legume starch granules by larvae of Zabrotes subfasciatus (Coleoptera: Bruchidae) and the induction of α–amylases in response to different diets

Silva, Carlos P.; Terra, Walter R.; Xavier‐Filho, J.; Sá, Maria Fátima Grossi de; Isejima, Eliza Mitiko; DaMatta, Renato Augusto; Miguens, Flávio Costa; Bifano, Thomas G.

doi:10.1016/s0965-1748(00)00103-x

Cited by 40 publications

(25 citation statements)

References 24 publications

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“…The factors inducing the production of enzymes in insects could be related to the amount of the appropriate substrates. For example, α-amylase induction in Zabrotes subfasciatus is related to the different starch granules from seeds of cowpea and the common bean (Silva et al, 2001). Sugars are important nutrients for caterpillars and other insects, and also universal phagostimulants (Schoonhoven and van Loon, 2002).…”

Section: Archives Of Insect Biochemistry and Physiology June 2008mentioning

confidence: 99%

Diet factors responsible for the change of the glucose oxidase activity in labial salivary glands of Helicoverpa armigera

Leung

Kang

et al. 2008

Arch Insect Biochem Physiol

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We investigated the change of the glucose oxidase (GOX) activity in labial salivary glands of Helicoverpa armigera larvae fed with the artificial diet or host plant tobacco and the major factors responsible for such a change. Throughout larval development, the labial salivary GOX activities in caterpillars reared on the artificial diet were remarkably higher than those fed with the plant. After fifth-instar plant-fed caterpillars were transferred to the artificial diet, their labial salivary GOX activity increased quickly, which was closely correlated with the time spent feeding on the artificial diet. The total sugar content of the artificial diet was 68 times higher than that of the tobacco leaves. We hypothesized that sugars and secondary metabolites are the possible causes of induction of GOX activity. When fifth-instar caterpillars were fed with tobacco leaves coated with glucose or sucrose, their labial salivary GOX activity was significantly higher than those fed with leaves without sugar coating. Following native PAGE, 1 single band of the labial salivary GOX was observed in all the caterpillars fed with different diets, implying that only the activity of the isoenzyme was changed in response to different diets. Furthermore, the labial salivary GOX activity was determined after caterpillars were fed with artificial diets containing chlorogenic acid, rutin, and quercetin. The results showed that all these phenolic compounds had no effect on the GOX activity. We conclude that sugar in diets was a major factor influencing the labial salivary GOX activity of the larvae. Arch. Insect Biochem. Physiol. 2008.

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Section: Archives Of Insect Biochemistry and Physiology June 2008mentioning

confidence: 99%

Diet factors responsible for the change of the glucose oxidase activity in labial salivary glands of Helicoverpa armigera

Leung

Kang

et al. 2008

Arch Insect Biochem Physiol

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“…Various insect amylase isoforms are known to have specificity of expression in different tissues and body fluids such as oral secretions and haemolymph (Da Lage et al, 2003;Parthasarathy and Gopinathan, 2005;Saltzmann et al, 2006). Variations in enzyme levels can also be due to presence of enzyme inhibitors, variations in monomers of complex macromolecules and when the concentrations and ratios of nutrients absorbed from natural diets are unknown (Clissold et al, 2010;Silva et al, 2001). Reducing sugars present in the diet reflected to the levels of reducing sugars occurring in the gut indicating their ready availability for absorption from plants food sources.…”

Section: Discussionmentioning

confidence: 99%

Amylase gene expression patterns in Helicoverpa armigera upon feeding on a range of host plants

Kotkar¹,

Bhide²,

Gupta³

et al. 2012

Gene

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“…A first example of introduction of plant genes encoding toxic protein against insect is cowpea trypsin inhibitor which is expressed in tobacco leaves to combat lepidopteran larvae (Hilder et al 1987;Silva et al 2001). Since then, attempts have been made to use toxic plant proteins against insects with some success.…”

Section: Introductionmentioning

confidence: 99%

“…Since then, attempts have been made to use toxic plant proteins against insects with some success. α-Amylase inhibitor gene from seeds of common bean (Phaseolus vulgaris) when transferred to pea confers resistance to pea weevil (Bruchus pisorum Linnaeus) (Coleoptera: Bruchidae) (Morton et al 2000;Silva et al 2001). Also, transformed Azuki bean (Vigna angularis) confers resistance to Callosobruchus chinensis Linnaeus (Coleoptera: Bruchidae) and C. maculatus Fabricius (Coleoptera: Bruchidae) (Ishimoto et al 1996;Silva et al 2001).…”

Section: Introductionmentioning

confidence: 99%

The effect of cereal seed extracts on amylase activity of the rose sawfly,Arge rosaeLinnaeus (Hymenoptera: Argidae)

Mohammadzadeh

Bandani

Borzoui

2013

Archives Of Phytopathology And Plant Protection

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The sawfly, Arge rosae Linnaeus (Hymenoptera: Argidae) is a serious pest of rose plants in a vast area of the world including Iran. Pesticides are applied in order to control in areas where infestation is high. So, there is a need for more environmentally benign alternatives to control this insect pest in urban areas. Therefore, the aim of the current study was to study the effect of cereal seed proteinaceous extracts including wheat and triticale on the insect α-amylase. Wheat and triticale seed proteins as well as fourth instars larvae α-amylase were extracted. The results showed that there are two different α-amylase isoenzymes in the insect gut, one major band and the other minor band, which had optimal activity at alkaline pH (pH 8.0). The effect of five concentrations including 0.42, 0.21, 0.105, 0.05 and 0.025 mg ml À1 of each seed extract was tested on α-amylase activity of the larvae gut. At the highest concentration (0.42 mg ml À1 ), wheat seed extract caused 74% inhibition of the enzyme activity while triticale seed extract inhibited 62% enzyme activity. Experiments proved that seed proteinaceous extract affected the enzyme activity in a pH-dependant manner.

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Digestion of legume starch granules by larvae of Zabrotes subfasciatus (Coleoptera: Bruchidae) and the induction of α–amylases in response to different diets

Cited by 40 publications

References 24 publications

Diet factors responsible for the change of the glucose oxidase activity in labial salivary glands of Helicoverpa armigera

Diet factors responsible for the change of the glucose oxidase activity in labial salivary glands of Helicoverpa armigera

Amylase gene expression patterns in Helicoverpa armigera upon feeding on a range of host plants

The effect of cereal seed extracts on amylase activity of the rose sawfly,Arge rosaeLinnaeus (Hymenoptera: Argidae)

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