Digestion of butyrate glycerides by pancreatic lipase

Sampugna, J.; Quinn, James G.; Pitas, Robert E.; Carpenter, D. L.; Jensen, Robert G.

doi:10.1007/bf02531853

Cited by 46 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A notable result from the current study was the significant decrease in the ratio of abdominal fat weight to the final body weight without any differences in feed intake, indicating a decrease of relative fat deposition in BG-fed chickens. Given that butyrate can be released from BG through lipases in the small intestine [ 27 ], we speculate that the decreased fat deposition could be achieved through the redistribution of ingested energy induced by butyrate acid. However, much remains to be learned about how butyrate (or BG) regulates the balance between synthesis, uptake, and transport of fatty acids.…”

Section: Discussionmentioning

confidence: 99%

“…In animals, butyric acid could be released from butyrate derivate feed additives, such as sodium butyrate and butyrate glycerides (BG), in the gastrointestinal tract [ 25 ]; however, in practice, butyric acid and sodium butyrate could be immediately absorbed by the upper digestive tract [ 26 ], therefore limiting the delivery of a sufficient amount of butyric acid to the target tissues or organs for the initiation of its beneficial effects. Butyrate glycerides have no such limitation as they could be released only under the action of lipases in the small intestine [ 27 , 28 ]. Thus, the effectiveness of butyric acid would be improved when it is protected from the absorption in the upper tract.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome Analysis Reveals Regulation of Gene Expression for Lipid Catabolism in Young Broilers by Butyrate Glycerides

Yin

Lepp

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Background & AimsButyrate has been shown to potently regulate energy expenditure and lipid metabolism in animals, yet the underlying mechanisms remain to be fully understood. The aim of this study was to investigate the molecular mechanisms of butyrate (in the form of butyrate glycerides, BG)-induced lipid metabolism at the level of gene expression in the jejunum and liver of broilers.Methodology/Principal FindingsTwo animal experiments were included in this study. In Experiment 1, two hundred and forty male broiler chickens were equally allocated into two groups: 1) basal diet (BD), 2) BG diets (BD + BG). Growth performance was compared between treatments for the 41-day trial. In Experiment 2, forty male broiler chickens were equally allocated into two groups. The general experimental design, group and management were the same as described in Experiment 1 except for reduced bird numbers and 21-day duration of the trial. Growth performance, abdominal fat deposition, serum lipid profiles as well as serum and tissue concentrations of key enzymes involved in lipid metabolism were compared between treatments. RNA-seq was employed to identify both differentially expressed genes (DEGs) and treatment specifically expressed genes (TSEGs). Functional clustering of DEGs and TSEGs and signaling pathways associated with lipid metabolism were identified using Ingenuity Pathways Analysis (IPA) and DAVID Bioinformatics Resources 6.7 (DAVID-BR). Quantitative PCR (qPCR) assays were subsequently conducted to further examine the expression of genes in the peroxisome proliferator-activated receptors (PPAR) signaling pathway identified by DAVID-BR. Dietary BG intervention significantly reduced abdominal fat ratio (abdominal fat weight/final body weight) in broilers. The decreased fat deposition in BG-fed chickens was in accordance with serum lipid profiles as well as the level of lipid metabolism-related enzymes in the serum, abdominal adipose, jejunum and liver. RNA-seq analysis indicated that dietary BG intervention induced 79 and 205 characterized DEGs in the jejunum and liver, respectively. In addition, 255 and 165 TSEGs were detected in the liver and jejunum of BG-fed group, while 162 and 211 TSEGs genes were observed in the liver and jejunum of BD-fed birds, respectively. Bioinformatic analysis with both IPA and DAVID-BR further revealed a significant enrichment of DEGs and TSEGs in the biological processes for reducing the synthesis, storage, transportation and secretion of lipids in the jejunum, while those in the liver were for enhancing the oxidation of ingested lipids and fatty acids. In particular, transcriptional regulators of THRSP and EGR-1 as well as several DEGs involved in the PPAR-α signaling pathway were significantly induced by dietary BG intervention for lipid catabolism.ConclusionsOur results demonstrate that BG reduces body fat deposition via regulation of gene expression, which is involved in the biological events relating to the reduction of synthesis, storage, transportation and secretion, and improvement o...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Regulation of Gene Expression for Lipid Catabolism in Young Broilers by Butyrate Glycerides

Yin

Lepp

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…It has been reported that pancreatic lipase displays an intermolecular rather than an intramolecular specificity (Sampugna et al 1967). These workers showed that release of short-and long-chain fatty acids from triglycerides containing both types of fatty acids occurred at similar rates, and also that fatty acid release was more rapid than was observed from triglycerides containing exclusively long-chain fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Relative Importance of Pancreatic Lipase and Pregastric Esterase on Lipid Absorption in Calves 1-2 Weeks of Age

Gooden¹,

Lascelles²

1973

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

An attempt has been made to determine the relative importance of pancreatic lipase and pregastric esterase on lipid absorption in calves 1-2 weeks of age. This was carried out by measuring the quantity of esterified lipid in lymph and the products of lipid hydrolysis in jejunal contents of 10 calves fed either milk or fatty whey in the presence or absence of pancreatic juice.The results showed that lipid was absorbed somewhat less efficiently during the feeding of fatty whey than milk and that a substantial reduction in lipid absorption was associated with the deprivation of pancreatic juice. It was also shown that malabsorption of lipid of calves deprived of pancreatic juice was almost entirely attributable to lipase deficiency rather than to a loss of buffering activity.The markedly reduced efficiency of lipid absorption in the absence of pancreatic juice, especially during fatty whey feeding, was associated with a low free fatty acid: triglyceride ratio of jejunal contents. The results suggested that the lipolytic activity remaining in the gastrointestinal tract in the absence of pancreatic juice was almost entirely attributable to pregastric esterase and that activity of the latter was substantially diminished once the digesta entered the intestine.

show abstract

“…The great advantage of monoglycerides is that organic acid is released from the glycerol backbone only under the action of intestinal lipases. This means that SCFA or MCFA remains protected from absorption in the upper gastrointestinal tract and could reach the final portion of intestine, where it would exert its major functions ( Sampugna et al, 1967 ; Namkung et al, 2011 ). Moreover, monoglycerides possess a more effective antimicrobial activity than the corresponding free fatty acids, since their efficacy is independent from environmental pH ( Bergsson et al, 2001 ; Sun, O’Connor & Roberton, 2003 ; Thormar, Hilmarsson & Bergsson, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of a specific composition of short- and medium-chain fatty acid 1-Monoglycerides on growth performances and gut microbiota of gilthead sea bream (Sparus aurata)

Rimoldi

Gliozheni

Ascione

et al. 2018

PeerJ

View full text Add to dashboard Cite

In aquaculture research, one important aim of gut microbiota studies is to provide the scientific basis for developing effective strategies to manipulate gut microbial communities through the diet, promoting fish health and improving productivity. Currently, there is an increasing commercial and research interest towards the use of organic acids in aquafeeds, due to several beneficial effects they have on growth performance and intestinal tract’s health of farmed fish. Among organic acids, monoglycerides of short-chain fatty acids (SCFAs) and medium-chain fatty acids (MCFAs) have attracted particular research attention also for their bacteriostatic and bactericidal properties. Accordingly, the present study aimed to evaluate the potential beneficial effects of SCFA and MCFA monoglycerides, used as a feed additive, on fish growth performance, and intestinal microbiota composition. For this purpose, a specific combination of short- and medium-chain 1-monoglycerides (SILOhealth 108Z) was tested in 600 juvenile gilthead sea bream (Sparus aurata) of about 60 g mean initial weight that were fed for 90 days with plant-based diets. Two isoproteic and isolipidic diets were formulated. The control fish group received a plant-based diet, whereas the other group received the same control feed, but supplemented with 0.5% of SILOhealth 108Z. The Illumina MiSeq platform for high-throughput amplicon sequencing of 16S rRNA gene and QIIME pipeline were used to analyse and characterize the whole microbiome associated both to feeds and S. aurata intestine. The number of reads taxonomically classified according to the Greengenes database was 394,611. We identified 259 OTUs at 97% identity in sea bream fecal samples; 90 OTUs constituted the core gut microbiota. Firmicutes, Proteobacteria and Actinobacteria represented the dominant phyla in both experimental groups. Among them, relative abundance of Firmicutes and Proteobacteria were positively and negatively affected by dietary SCFA monoglycerides supplementation, respectively. In summary, our findings clearly indicated that SILOhealth 108Z positively modulated the fish intestinal microbiota by increasing the number of beneficial lactic acid bacteria, namely, Lactobacillus, and reducing Gammaproteobacteria, which include several potential pathogenic bacteria. The specific composition of 1-monoglycerides of short- and medium-chain fatty acids contained in SILOhealth 108Z could thus have a great potential as a feed additive in aquaculture.

show abstract

Digestion of butyrate glycerides by pancreatic lipase

Cited by 46 publications

References 21 publications

Transcriptome Analysis Reveals Regulation of Gene Expression for Lipid Catabolism in Young Broilers by Butyrate Glycerides

Transcriptome Analysis Reveals Regulation of Gene Expression for Lipid Catabolism in Young Broilers by Butyrate Glycerides

Relative Importance of Pancreatic Lipase and Pregastric Esterase on Lipid Absorption in Calves 1-2 Weeks of Age

Effect of a specific composition of short- and medium-chain fatty acid 1-Monoglycerides on growth performances and gut microbiota of gilthead sea bream (Sparus aurata)

Contact Info

Product

Resources

About