Digestion characteristics of quinoa, barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC-1 cells

Song, Hongdong; Fu, Qiuyun; Huang, Kai; Zou, Zhiying; Chen, Limin; Chen, Hulin; Ge, Shaocheng; Wang, Jing; Guan, Xiao

doi:10.1039/d2fo00243d

Cited by 12 publications

(17 citation statements)

References 46 publications

(70 reference statements)

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“…Geraedts and co‐workers found that egg protein and casein hydrolysates had a significantly different ability to stimulate CCK secretion 30 . Our previous study also found that barley protein digest exhibited a potent CCK secretagogue activity, but the digests from quinoa and mungbean proteins had only a very weak effect on CCK secretion 7 . These studies suggested that different dietary proteins and degree of hydrolysis significantly affect the CCK secretagogue activity of protein hydrolysates or peptides.…”

Section: Discussionsupporting

confidence: 51%

“…reported that soybean protein hydrolysate stimulated CCK secretion and inhibited feed intake through CaSR 6 . Our previous study also found that CaSR was involved in barley protein digest‐induced CCK secretion 7 . However, it remains unclear whether CaSR is involved in wheat protein digest (WPD)‐mediated CCK secretion.…”

Section: Introductionmentioning

confidence: 93%

“…Different dietary proteins and degree of hydrolysis significantly affect the CCK secretagogue activity of protein hydrolysates or peptides. Our previous study found that the simulated digest of barley protein exerted stronger CCK‐stimulatory activity in STC‐1 cells than that of mungbean and quinoa proteins at the same treatment dose 7 . Santos‐Hernández and co‐workers reported that the digest fraction from the intestinal phase had a stronger CCK‐stimulatory activity than that from the gastric phase 12 .…”

Section: Introductionmentioning

confidence: 98%

“…They have a great potential for use in functional foods or nutraceuticals 4 . Currently, some bioactive peptides or protein hydrolysates from milk whey, 5 soybean, 6 barley 7 and egg white 8 have been demonstrated to have CCK secretagogue activities, indicating that bioactive peptides have a great potential to combat obesity by stimulating CCK secretion. However, knowledge about the stimulatory effect of other bioactive peptides or protein hydrolysates on CCK secretion is still limited.…”

Section: Introductionmentioning

confidence: 99%

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Identification and target of action of cholecystokinin‐releasing peptides from simulated digestion hydrolysate of wheat protein

Song,

Wang,

Shao

et al. 2023

J Sci Food Agric

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BackgroundWheat protein intake leads to improved appetite control. However, the active components causing appetite in wheat have not fully clarified. Gut cholecystokinin (CCK) plays a vital role in appetite control. This study aimed to investigate the ability of wheat protein digest (WPD) to stimulate CCK secretion and clarify the active components and target of action.ResultsWPD was prepared by simulated gastrointestinal digestion model. WPD treatment with a concentration of 5 mg/mL significantly stimulated CCK secretion in enteroendocrine STC‐1 cells (P < 0.05). Furthermore, oral gavage with WPD in mice significantly increased plasma CCK level at 60 min (P < 0.01). Preparative C18 column separation was used to isolate peptide fractions associated with CCK secretion and peptide sequences were identified by LC‐MS/MS. A new CCK‐releasing peptide RYIVPL that could potently stimulated CCK secretion was successfully identified. After pretreatment with a specific calcium‐sensing receptor (CaSR) antagonist NPS 2143, CCK secretion induced by WPD or RYIVPL was greatly suppressed, suggesting that CaSR was involved in WPD or RYIVPL induced CCK secretion.ConclusionThe present study demonstrated that WPD had an ability to stimulate CCK secretion in vitro and in vivo, and determined that peptide RYIVPL in WPD could stimulate CCK secretion through CaSR.This article is protected by copyright. All rights reserved.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and target of action of cholecystokinin‐releasing peptides from simulated digestion hydrolysate of wheat protein

Song,

Wang,

Shao

et al. 2023

J Sci Food Agric

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“…Traditionally, proteins are considered the most satiating macronutrient compared with an isoenergetic intake of carbo-hydrates and fat, 5 suggesting that dietary proteins are potential potent stimulants of CCK secretion. Several protein hydrolysates or digests from soybean, 6 milk whey, 7 barley, 8 and egg white 9 have been demonstrated to stimulate CCK secretion. However, the knowledge about CCK secretagogue activity induced by other protein hydrolysates or digests is limited.…”

Section: Introductionmentioning

confidence: 99%

In vitrogastrointestinal digestion of buckwheat (Fagopyrum esculentumMoench) protein: release and structural characteristics of novel bioactive peptides stimulating gut cholecystokinin secretion

et al. 2023

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In vitro gastrointestinal digestion of highland barley protein: identification and characterization of novel bioactive peptides involved in gut cholecystokinin secretion

et al. 2023

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BACKGROUNDThe satiety hormone cholecystokinin (CCK) plays an important role in food intake inhibition. Its secretion is regulated by dietary components. The search for bioactive compounds that induce CCK secretion is currently an active area of research. The objective of this study was to evaluate the ability of highland barley protein digest (HBPD) to stimulate CCK secretion in vitro and in vivo and identify the responsible peptide sequences.RESULTSHBPD was prepared by in vitro gastrointestinal digestion model. Peptides of <1000 Da in HBPD accounted for 82%. HBPD was rich in essential amino acids Leu, Phe and Val, but lack in sulfur amino acids Met and Cys. HBPD treatment at a concentration of 5 mg mL−1 significantly stimulated CCK secretion from enteroendocrine STC‐1 cells (P < 0.05). Moreover, oral gavage with HBPD in mice significantly increased plasma CCK level. Chromatographic separation was performed to isolate peptide fractions involved in CCK secretion and peptide sequence was determined by ultra‐performance liquid chromatography–tandem mass spectrometry. Two novel CCK‐releasing peptides, PDLP and YRIVPL, were pointed out for their outstanding CCK secretagogue activity.CONCLUSIONThis study demonstrated for the first time that HBPD had an ability to stimulate CCK secretion in vitro and in vivo and determined the bioactive peptides exerting CCK secretagogue activity in HBPD. © 2023 Society of Chemical Industry.

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Digestion characteristics of quinoa, barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC-1 cells

Abstract: The demand for plant-based proteins has been rapidly increasing due to sustainability, ethical and health reasons. The present study aimed to investigate the digestion characteristics of three plant proteins (quinoa,...

Cited by 12 publications

References 46 publications

Identification and target of action of cholecystokinin‐releasing peptides from simulated digestion hydrolysate of wheat protein

Identification and target of action of cholecystokinin‐releasing peptides from simulated digestion hydrolysate of wheat protein

In vitrogastrointestinal digestion of buckwheat (Fagopyrum esculentumMoench) protein: release and structural characteristics of novel bioactive peptides stimulating gut cholecystokinin secretion

In vitro gastrointestinal digestion of highland barley protein: identification and characterization of novel bioactive peptides involved in gut cholecystokinin secretion

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