DIGE analysis of proteome changes accompanying large resveratrol production by grapevine (Vitis vinifera cv. Gamay) cell cultures in response to methyl-β-cyclodextrin and methyl jasmonate elicitors

Martínez-Esteso, María José; Sellés-Marchart, Susana; Vera-Urbina, J.C.; Pedreño, María A.; Bru-Martínez, Roque

doi:10.1016/j.jprot.2011.02.035

Cited by 38 publications

(56 citation statements)

References 63 publications

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“…Monastrell. These results correlate well with those obtained by Martinez-Esteso et al [58] and by Lijavetzky et al [22], respectively. …”

Section: Pol Scientificsupporting

confidence: 92%

“…One of the bottlenecks of making Vitis cell culture technology viable to produce t-R has been the establishment of suitable elicitation conditions [21,22,58]. Hitherto, constitutive promoters are generally used to study the effects of transgene expression in plants but, given the fact that constitutive gene expression may be harmful to the host plant, including yield penalty [70,71 and references therein], the effect of GFP constitutive expression on t-R production by elicited grapevine cell suspension was assessed.…”

Section: Discussionmentioning

confidence: 99%

“…Our group determined that the elicitation treatments of cell suspensions V. vinifera cv.Gamay and cv. Monastrell with MBCD combined with MeJA led to major increases in t-R accumulation [22,58] because t-R was synthesised abundantly in treated cells and translocated continuously to the medium. However, treatment with MBCD+MeJA did not lead to the de novo synthesis of c-R, which was a minority metabolite compared to t-R. Figure 5 shows the amount of accumulated t-R in extracellular medium after 72 h of incubation with elicitors MBCD and MeJA, which were quantitatively determined by HPLC.…”

Section: Pol Scientificmentioning

confidence: 99%

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A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

et al. 2015

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One of the major intent of metabolic engineering in cell culture systems is to increase yields of secondary metabolites. Efficient transformation methods are a priority to successfully apply metabolic engineering to cell cultures of plants that produce bioactive or therapeutic compounds, such as Vitis vinifera and Taxus x media. The aim of this study was to establish a reliable method to transform non-embryogenic cell cultures of these species. The V. vinifera cv. Gamay/cv. Monastrell cell lines and Taxus x media were used for Agrobacterium-mediated transformation using the Gateway-compatible Agrobacterium sp. binary vector system for fast reliable DNA cloning. The Taxus x media and Vitis cell lines were maintained in culture for more than 4 and 15 months, respectively, with no loss of reporter gene expression or antibiotic resistance. The introduced genes had no discernible effect on cell growth, or led to extracellular accumulation of phytoalexin trans-Resveratrol (t-R) in response to elicitation with methylated cyclodextrins (MBCD) and methyl jasmonate (MeJA) in the grapevine transgenic cell lines compared to the parental control. The method described herein provides an excellent tool to exploit exponentially growing genomic resources to enhance, optimize or diversify the production of bioactive compounds generated by grapevine and yew cell cultures, and offers a better understanding of many grapevine and yew biology areas.

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“…Monastrell. These results correlate well with those obtained by Martinez-Esteso et al [58] and by Lijavetzky et al [22], respectively. …”

Section: Pol Scientificsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Pol Scientificmentioning

confidence: 99%

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A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

et al. 2015

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“…MeJA treatment of grape cell suspension produces a direct effect on the stilbene synthesis of both free RESV and mono-glucoside derivatives and of anthocyanins (Krisa et al, 1999;Tassoni et al, 2005;Belhadj et al, 2008;Lijavetzky et al, 2008;Faurie et al, 2009;Martinez-Esteso et al, 2011). In our previous work on Barbera petiole cells treated with 10 M MeJA, a maximum of production of trans-RESV was measured at day 2 of culture (Tassoni et al, 2005).…”

Section: Discussionmentioning

confidence: 93%

Combined elicitation of methyl-jasmonate and red light on stilbene and anthocyanin biosynthesis

Tassoni

Durante

Ferri

2012

Journal of Plant Physiology

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“…Piceid has also been assessed in 'Gamay' Vitis viniferacell suspensions with the highest values found of 280 mg L −1 (Aumont et al, 2004). It is the most abundant stilbenoid and accumulates with the biomass in normal growth medium (Martínez-Esteso et al, 2011). When elicitors are added, resveratrol becomes highly abundant and is released into the culture medium (Morales et al, 1997;Bru et al, 2006;Donnez et al, 2009;Martínez-Esteso et al, 2009;.…”

Section: Cell Aggregates From the Suspensions Culturementioning

confidence: 99%

Callus Induction and Cellular Suspensions from Murtilla (<i>Ugni molinae</i> Turcz.) for <i>trans</i>-resveratrol Production

Carolina¹,

Evelyn²,

Claudia³

et al. 2017

American Journal of Biochemistry and Biotechnology

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The present study reports for the first time the quantification of resveratrol and the use of biotechnological techniques applied to stilbene productions in species from genus Ugni and calli production from adult explants (fruits). Resveratrol is synthesized by a series of families of higher plants, which has generated much interest in recent years for its antioxidant, anticancer and antitumor properties, which would allow longevity of cells to be prolonged. In this study, leaves and mature fruits were collected from three sites in Southern Chile and correspond to three ecotypes of the Chilean endemic species Ugni molinae Turz. (murtilla). These were established in vitro to prepare the callus and subsequent development of cellular suspensions for trans-resveratrol production. Our results showed that these stilbenes are present in murtilla and that their concentrations vary between ecotypes and tissues, reaching up to 553.5 µg g −1 of t-resveratrol produced in ecotype 3 callus. These values are relatively higher than those found in other plant species. Under optimum culture conditions, extraction of resveratrol from Ugni molinae is scalable to industrial levels, which makes it a viable alternative for obtaining stilbenes.

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DIGE analysis of proteome changes accompanying large resveratrol production by grapevine (Vitis vinifera cv. Gamay) cell cultures in response to methyl-β-cyclodextrin and methyl jasmonate elicitors

Cited by 38 publications

References 63 publications

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

Combined elicitation of methyl-jasmonate and red light on stilbene and anthocyanin biosynthesis

Callus Induction and Cellular Suspensions from Murtilla (<i>Ugni molinae</i> Turcz.) for <i>trans</i>-resveratrol Production

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