Difructose Anhydrides-Producing Fructotransferase: Characteristics, Catalytic Mechanism, and Applications

Cheng, Mei; Zhu, Yingying; Mu, Wanmeng

doi:10.1007/978-981-33-6021-1_8

Cited by 2 publications

(2 citation statements)

References 112 publications

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“…GH91 family enzymes use an inverting mechanism and share with a parallel β-helix three-dimensional structure status. 26 The difference in glycosidic linkage in the products (DFA I and DFA III) of inulin fructotransferases is still an intriguing question and remains open. This encouraged us to investigate IFTase I from a structural view.…”

Section: ■ Discussionmentioning

confidence: 99%

Structural Insights into the Catalytic Cycle of Inulin Fructotransferase: From Substrate Anchoring to Product Releasing

Cheng,

Hou,

Huang

et al. 2024

J. Agric. Food Chem.

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Carbohydrate degradation is crucial for living organisms due to their essential functions in providing energy and composing various metabolic pathways. Nevertheless, in the catalytic cycle of polysaccharide degradation, the details of how the substrates bind and how the products release need more case studies. Here, we choose an inulin fructotransferase (SpIFTase) as a model system, which can degrade inulin into functionally difructose anhydride I. At first, the crystal structures of SpIFTase in the absence of carbohydrates and complex with fructosyl-nystose (GF4), difructose anhydride I, and fructose are obtained, giving the substrate trajectory and product path of SpIFTase, which are further supported by steered molecular dynamics simulations (MDSs) along with mutagenesis. Furthermore, structural topology variations at the active centers of inulin fructotransferases are suggested as the structural base for product release, subsequently proven by substitution mutagenesis and MDSs. Therefore, this study provides a case in point for a deep understanding of the catalytic cycle with substrate trajectory and product path.

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Section: ■ Discussionmentioning

confidence: 99%

Structural Insights into the Catalytic Cycle of Inulin Fructotransferase: From Substrate Anchoring to Product Releasing

Cheng,

Hou,

Huang

et al. 2024

J. Agric. Food Chem.

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“…Thus, this confirms that DPA was derived from ᴅ -psicose. DFAs are generated through thermal and/or acidic treatments, and are mostly made from substances that exist in nature, such as inulin and levan [ 7 , 12 , 13 , 14 ]. However, the polymerization of ᴅ -psicose remains a huge challenge.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of a New Glycoconjugate with Di-ᴅ-Psicose Anhydride Structure

Jung

Kim

Lim

et al. 2022

IJMS

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Demand for healthy diets has led researchers to explore new saccharide as sucrose alternatives. D-Psicose, the C-3 epimer of D-fructose, has a similar sweetness intensity to sucrose but contributes fewer calories. This study proposes a disaccharide with a stable structure derived from D-psicose. The compound with a spiro-tricyclic core was generated at 32% conversion via caramelization of D-psicose under acidic anhydrous conditions. The compound was identified by high-resolution mass spectrometry and multi-dimensional nuclear magnetic resonance (NMR). The molecular formula was established as C12H20O10 from the molecular weight of m/z 324.1055. Twelve signals were observed by the 13C NMR spectrum. This compound, denoted di-D-psicose anhydride (DPA), exhibited a lower water solubility (40 g/L) and higher thermal stability (peak temperature = 194.7 °C) than that of D-psicose (peak temperature = 126.5 °C). The quantitatively evaluated metal ion scavenging ability of DPA was the best in magnesium (average 98.6 ± 1.1%). This synthesis methodology can provide disaccharides with high stability-reducing heavy metals.

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Difructose Anhydrides-Producing Fructotransferase: Characteristics, Catalytic Mechanism, and Applications

Cited by 2 publications

References 112 publications

Structural Insights into the Catalytic Cycle of Inulin Fructotransferase: From Substrate Anchoring to Product Releasing

Structural Insights into the Catalytic Cycle of Inulin Fructotransferase: From Substrate Anchoring to Product Releasing

Synthesis of a New Glycoconjugate with Di-ᴅ-Psicose Anhydride Structure

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